公开(公告)号：WO2002082051A2

公开(公告)日：2002-10-17

申请号：PCT/US2002/001541

申请日：2002-01-15

Applicant: TUBBS, Kemmons, A.

Inventor： TUBBS, Kemmons, A. ,  NELSON, Randall, W. ,  GRUBER, Karl, A.

IPC: G01N

CPC classification number: G01N33/6845 ,  B01J20/28097 ,  B01J20/281 ,  B01J20/286 ,  B01J20/32 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3242 ,  B01J20/3259 ,  B01J20/3265 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3282 ,  B01J2220/54 ,  B01J2220/64 ,  B82Y30/00 ,  G01N30/02 ,  G01N30/48 ,  G01N30/6095 ,  G01N33/54366 ,  G01N33/545 ,  G01N33/6803 ,  G01N2570/00 ,  H01J49/164 ,  H01J49/40 ,  Y10T436/255 ,  B01D15/3804

Abstract: The present invention provides an integrated system capable of selectively retrieving and concentrating specific biomolecules from biological media for subsequent high-performance analyses, quantifying targeted proteins, recognizing variants of targeted biomolecules (e.g., splice variants, point mutations and posttranslational modifications) and elucidating their nature, analyzing for, and identifying, ligands interacting with targeted biomolecules, and high-throughput screening of large populations of samples using a single, unified, economical, multiplexed and parallel processing platform. The prefer-red embodiment of the integrated system comprises molecular traps, such as affinity microcolumns, derivatized mass spectrometer targets, mass spectrometers capable of multi-sample input and robotics with processing/data analysis interactive database. The present invention also includes methods and processes for use of the individual components and the integrated system in biological applications. Furthermore, the preferred embodiment of the present invention provides for the preparation and/or processing of multiple separate devices and/or samples to accomplish high throughput analysis.

Abstract translation: 本发明提供了能够选择性地从生物培养基中回收和浓缩特定生物分子以进行后续高效分析，定量靶向蛋白质，识别靶向生物分子变异（例如，剪接变体，点突变和翻译后修饰）并阐明其性质的整合系统 ，分析和鉴定与靶向生物分子相互作用的配体，以及使用单个，统一，经济，多路复用和并行处理平台的大量样品的高通量筛选。 集成系统的优选实施例包括分子阱，例如亲和力微柱，衍生质谱仪目标，能够进行多样本输入的质谱仪和具有处理/数据分析交互式数据库的机器人。 本发明还包括在生物应用中使用各个部件和集成系统的方法和方法。 此外，本发明的优选实施例提供了用于实现高通量分析的多个分离的装置和/或样品的制备和/或处理。