公开(公告)号：WO2015019336A2

公开(公告)日：2015-02-12

申请号：PCT/IB2014/064005

申请日：2014-08-21

Applicant: UNIVERSITEIT LEIDEN

Inventor： VULTO, Paul ,  TRIETSCH, Sebastiaan, Johannes ,  YILDIRIM, Ender

CPC classification number: F16K99/0026 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2400/0406 ,  B01L2400/0688 ,  F16K2099/0084 ,  Y10T137/7859

Abstract: The present invention relates to a fluid triggerable passive valve for controlling flow of one or more fluids comprising: a volume comprising at least two capillary pressure barriers for respectively pinning a first and second fluid-fluid meniscus, the two capillary pressure barriers dividing the volume in at least three sub volumes, whereby two sub volumes being positioned upstream and one sub volume being positioned downstream with respect to the advancement direction of each respective fluid-fluid meniscus; wherein the capillary pressure barriers are spaced apart such that upon pinning of a first fluid-fluid meniscus on a first capillary pressure barrier and arrival or pinning of a second fluid-fluid meniscus on or at a second capillary pressure barrier, the two menisci touch and thereby coalesce into one fluid-fluid meniscus

Abstract translation: 本发明涉及一种用于控制一种或多种流体流动的流体可触发的被动阀，包括：一个体积，包括至少两个用于分别固定第一和第二流体 - 液体弯液面的毛细管压力屏障，两个毛细管压力屏障将体积分成 至少三个子体积，其中两个子体积位于上游，一个子体积相对于每个相应的流体 - 液体弯液面的前进方向位于下游; 其中所述毛细管压力屏障间隔开，使得当在第一毛细管压力屏障上锁定第一流体 - 液体弯液面并且在第二毛细管压力屏障上或在第二毛细管压力屏障上到达或钉住第二流体 - 液体弯月面时，两个半月板触摸和 从而聚结成一个流体 - 液体弯液面

公开(公告)号：WO2014204312A2

公开(公告)日：2014-12-24

申请号：PCT/NL2014/050406

申请日：2014-06-19

Applicant: UNIVERSITEIT LEIDEN

Inventor： SCHOONEN, Jan-Willem ,  LINDENBURG, Peter ,  VULTO, Paul ,  HANKEMEIER, Thomas

IPC: G01N1/28

CPC classification number: G01N30/12 ,  G01N1/28 ,  G01N1/34 ,  G01N1/4022 ,  G01N30/06 ,  G01N30/463 ,  G01N30/64 ,  G01N30/7233

Abstract: The present invention relates to a droplet receiver, comprising a receptacle for receiving a droplet, a fluid conduit connected at a first end thereof to the droplet receptacle thereby providing a course within which the droplet received in the droplet receptacle moves, the conduit at its distal end in communication with a reservoir,and a fluid layer wetting the internal walls of the receptacle and conduit in such a way as permit reception of the droplet without loss of the received droplet due to wetting of the internal receptacle and conduit walls.

Abstract translation: 本发明涉及一种液滴接收器，包括用于接收液滴的容器，在其第一端处连接到液滴容器的流体导管，从而提供容纳在液滴容器中的液滴移动的过程，其远端处的导管 与储存器连通，并且液体层以允许接收液滴而不会由于内部容器和导管壁的润湿而损失所接收的液滴的方式润湿容器和导管的内壁。

公开(公告)号：WO2012120101A4

公开(公告)日：2012-09-13

申请号：PCT/EP2012/054053

申请日：2012-03-08

Applicant: UNIVERSITEIT LEIDEN ,  VULTO, Paul ,  TRIETSCH, Sebastiaan Johannes ,  VAN DER LINDEN, Heiko Jan ,  HANKEMEIER, Thomas

Inventor： VULTO, Paul ,  TRIETSCH, Sebastiaan Johannes ,  VAN DER LINDEN, Heiko Jan ,  HANKEMEIER, Thomas

IPC: B01L3/00 ,  B01F13/00 ,  F16K99/00

Abstract: Apparatus for processing liquids or liquid-based substances includes a plurality of volumes at least two of which are defined at least in part by one or more phaseguides inside the volume and/or in a conduit connected thereto for controlling aliquoting of one or more liquids or liquid-based substances inside the volume. Each volume has an upstream and downstream side with respect to meniscus advancement direction via which it may be filled with or emptied of one or more liquids or liquid-based substances. The apparatus also includes at least one common upstream-side conduit connected to supply a liquid or liquid-based substance via a plurality of the inlet or extraction conduits, a plurality of the phaseguides exhibiting a predetermined level of stability and one or more of the phaseguides exhibiting a predetermined different stability compared with the stability of at least one of the other phaseguides whereby to control the preference order in which the volumes fill and/or empty. The stability is determined by the value and radius of an acute angle along a said phaseguide at the downstream side of the phaseguide.

公开(公告)号：WO2013137726A2

公开(公告)日：2013-09-19

申请号：PCT/NL2013/050163

申请日：2013-03-12

Applicant: UNIVERSITEIT LEIDEN

Inventor： HANKEMEIER, Thomas ,  SCHOONEN, Jan-Willem ,  VAN DER LINDEN, Heiko Jan ,  VULTO, Paul

IPC: G01N1/40

CPC classification number: G01N1/4022 ,  B01D1/0082 ,  G01N2001/4027

Abstract: The present invention relates to a device and an arrangement for selective solvent evaporation from a liquid feed, the feed comprising one or more components diluted in at least a first solvent or a solvent blend, comprising: a) a first tubular vessel having a distal end or a channel suitable for the formation of a droplet of a first volume, at an inflow rate n, at the tip or in the lumen of the tubular vessel, and b) means for subjecting the droplet to a solvent removal step at an evaporation rate r 2 to evaporate at least part of the first solvent or solvent blend, to accumulate the components in the feed in the droplet during the evaporation process at an accumulation rate r 3 , to obtain a concentrated feed volume in a concentrated droplet, wherein the evaporation and/or inflow rates are continuously adjusted to achieve a desired accumulation rate. The invention further relates to a method for selective solvent removal from an analyte mixture obtained in a chromatographic separation process.

Abstract translation: 本发明涉及一种用于从液体进料选择性溶剂蒸发的装置和装置，所述进料包含在至少第一溶剂或溶剂共混物中稀释的一种或多种组分，所述组分包括：a）第一管状容器，其具有远端 或适于在管状容器的尖端或内腔中以流入速率n形成第一体积的液滴的通道，以及b）使液滴以蒸发速率进行溶剂去除步骤的装置 r2蒸发至少部分第一溶剂或溶剂共混物，以在堆积速率r3的蒸发过程中将液体中的组分积聚在液滴中，以获得浓缩液滴中的浓缩进料体积，其中蒸发和/ 或流入速率连续调整以达到所需的累积率。 本发明还涉及从色谱分离过程中获得的分析物混合物中选择性溶剂去除的方法。

公开(公告)号：WO2012120102A1

公开(公告)日：2012-09-13

申请号：PCT/EP2012/054056

申请日：2012-03-08

Applicant: UNIVERSITEIT LEIDEN ,  KOPLA B.V. ,  VULTO, Paul ,  TRIETSCH, Sebastiaan Johannes ,  VAN DER LINDEN, Heiko Jan ,  JOORE, Adrianus Theodorus ,  HANKEMEIER, Thomas

Inventor： VULTO, Paul ,  TRIETSCH, Sebastiaan Johannes ,  VAN DER LINDEN, Heiko Jan ,  JOORE, Adrianus Theodorus ,  HANKEMEIER, Thomas

IPC: C12M3/00

CPC classification number: C12M27/18 ,  B01F13/0083 ,  B01L3/502746 ,  B01L3/56 ,  B01L2200/0605 ,  B01L2200/0621 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/0688 ,  B01L2400/086 ,  B01L2400/088 ,  C12M23/16 ,  C12M23/34 ,  C12M29/10 ,  C12M35/08 ,  Y10T137/0318

Abstract: Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume. The first sub-volume (14) contains one or more life-based particles supported in or by a gel or gel-like substance; and the second sub-volume (16) communicates with the first sub-volume so as to permit transport of substances between the first and second sub-volumes (14, 16) and contains at least one gel or gel-like substance.

Abstract translation: 用于处理基于生命的有机颗粒的装置，包括选自包括细胞，细胞球体，组织，真核生物，微生物，器官或胚胎的颗粒的颗粒，包括中空体积（10），其中（a）至少内部分为 第一（14），第二（16）和第三（17）子体积，形成在容积内形成的至少两个相位导体（12,13），（b）包括相对上游和相对下游的部分， 弯液面或大量液体在体积（10）中的移动。 该装置包括至少第一，第二和第三流体导管（19,21,22），其被连接以允许容积（10）的上游外部与相应的所述子体积（14,16,17）之间的流体连通。 以及至少一个另外的导管（24），其连接以允许容积（10）的下游外部与所述子体积之间的流体连通。 第一子体积（14）包含一种或多种在凝胶或凝胶状物质中或由凝胶或类似物质负载的基于生命的颗粒; 并且所述第二子体积（16）与所述第一子体积连通以允许物质在所述第一和第二子体积（14,16）之间运输并且包含至少一种凝胶或凝胶状物质。

公开(公告)号：WO2014204311A1

公开(公告)日：2014-12-24

申请号：PCT/NL2014/050405

申请日：2014-06-19

Applicant: UNIVERSITEIT LEIDEN

Inventor： SCHOONEN, Jan-Willem ,  LINDENBURG, Peter ,  VAN DUINEN, Vincent ,  OEDIT, Amar ,  VULTO, Paul ,  HANKEMEIER, Thomas

IPC: G01N1/40 ,  B01L3/00 ,  B01D11/04

CPC classification number: B01D11/0419 ,  B01D11/0496 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2300/0645 ,  G01N1/40 ,  G01N2001/4038 ,  G01N2001/4072

Abstract: The present invention relates to a process for the electro extraction of molecules from a moving fluid donor phase into an acceptor phase, comprising the steps of: providing an electrically conductive donor phase moving at a first flow velocity and in electrically conductive contact with a first electrode, providing an electrically conductive acceptor phase in direct contact and immiscible with the donor phase, in electrically conductive contact with a second electrode;and providing a supporting or confining phase guide pattern to keep a defined interface between donor phase and acceptor phase, and (d) applying an electrical field between the first and the second electrode.

Abstract translation: 本发明涉及一种将分子从移动的液体给体相电解提取到受体相中的方法，包括以下步骤：提供以第一流速移动并与第一电极导电接触的导电供体相 提供与供体相直接接触并不与其混溶的导电受体相，与第二电极导电接触;以及提供支撑或约束相导向图以保持供体相和受体相之间的界定，和（d ）在第一和第二电极之间施加电场。

公开(公告)号：WO2014038943A1

公开(公告)日：2014-03-13

申请号：PCT/NL2013/050650

申请日：2013-09-10

Applicant: UNIVERSITEIT LEIDEN

Inventor： VULTO, Paul ,  TRIETSCH, Sebastiaan Johannes ,  YILDIRIM, Ender

IPC: B01L3/00

CPC classification number: B01L3/00 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/161 ,  B01L2400/0688 ,  B01L2400/086 ,  B01L2400/088

Abstract: The present invention relates to an apparatus for controlling the shape and/or position of a moveable fluid-fluid meniscus, the apparatus comprising a volume for containing and directing fluid, the filling direction being a downstream direction, including the meniscus and the volume having at least a first structure defining a capillary pressure barrier along which the meniscus tends to align, the capillary pressure barrier and the meniscus defining a boundary in the volume between at least two sub-volumes,wherein (a) the capillary pressure barrier is stabilized by subtending at both ends an angle with a wall of the volume that on the downstream side of the capillary pressure barrier is greater than 90º, while not providing a deliberate fluid alignment weakness along the capillary pressure barrier that reduces the stability of the capillary pressure barrier and/or (b) wherein the capillary pressure is stabilized by providing a stretching barrier at a distance less than the maximum stretching distance of the fluid-fluid meniscus upon alignment along the capillary pressure barrier in the absence of the stretching barrier, (c) the capillary pressure barrier is stabilized by subtending at one end an angle with a wall of the volume that on the downstream side of the capillary pressure barrier is greater than 90º, and at the other end is stabilized by providing a stretching barrier at a distance less than the maximum stretching distance of the fluid-fluid meniscus upon alignment along the capillary pressure barrier in the absence of the stretching barrier; wherein the stretching barrier is shaped such that at least one directional component is orthogonal to the capillary pressure barrier.

Abstract translation: 本发明涉及一种用于控制可移动流体 - 液体弯液面的形状和/或位置的装置，该装置包括用于容纳和引导流体的容积，填充方向是下游方向，包括弯月面和体积具有 至少一个限定毛细管压力屏障的第一结构，所述弯月面倾向于沿其对准，所述毛细管压力屏障和所述弯液面在至少两个子体积之间限定所述体积中的边界，其中（a）毛细管压力屏障通过对 在两端处，与毛细管压力屏障的下游侧的体积壁成一角度大于90度，而不能沿着毛细管压力屏障提供有意的流体排列弱点，这降低了毛细管压力屏障的稳定性和/ 或（b）其中通过在小于最大值的距离处提供拉伸屏障来稳定毛细管压力 在不存在拉伸屏障的情况下沿着毛细管压力屏障对准流体 - 液体弯液面的蚀刻距离，（c）通过在一端与一个下游侧的体积的壁成一角度来稳定毛细管压力屏障 的毛细管压力屏障大于90度，另一端通过在不存在拉伸的情况下沿着毛细管压力屏障排列时在小于流体 - 液体弯液面的最大拉伸距离的距离处提供拉伸屏障而得到稳定 屏障; 其中所述拉伸屏障被成形为使得至少一个方向分量正交于所述毛细管压力屏障。

公开(公告)号：WO2014030997A1

公开(公告)日：2014-02-27

申请号：PCT/NL2013/050608

申请日：2013-08-21

Applicant: UNIVERSITEIT LEIDEN

Inventor： VULTO, Paul ,  HANKEMEIER, Thomas ,  QUIST, Jos ,  JANSSEN, Kjeld

IPC: G01N27/447

CPC classification number: G01N27/447 ,  G01N27/44773

Abstract: The present invention relates to amethod and an apparatus for concentrating, detecting and/orisolating a plurality of charged analytes contained in a sampleby depletion zone isotachophoresis.

Abstract translation: 本发明涉及用于通过耗尽区等速电泳浓缩，检测和/或分离包含在样品中的多个带电分析物的方法和装置。