Abstract:
Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.
Abstract:
A fluid gating valve includes a porous hydrophobic layer which acts a barrier for liquid flow; a liquid source on one side of the porous layer; a liquid drain on the opposing side of the porous hydrophobic layer; a source electrode in electrical connection with the liquid source; and a gate electrode electrically insulated from the liquid source, wherein the source electrode and the gate electrode are positioned and arranged to be connectable to a voltage source. And valve can be open by applying voltage between the gate and the source electrodes.
Abstract:
According to the invention there is a microfluidic chip (1) that includes at least two layers (10) forming a stack of layers, each layer of which has at least one flow channel (14); a bore (16) extending through the layers and communicating with a plurality of flow channels; and a valve (20), which has a shaft (22) with a recess (222) in a side of the shaft for fluid to flow through. The shaft is rotatably mounted in the bore, and has a first position in which the recess is aligned with each of at least two flow channels of the plurality of flow channels thereby providing a flow path between said at least two flow channels, and a second position in which the recess is unaligned with at least one of said at least two flow channels the flow path between said at least two flow channels thereby being closed. This allows a fluid flow path between two flow channels to be open and closed by rotation of the shaft so that fluid in the microfluidic chip can be redirected to allow the chip to have greater capability and by using a minimal amount of space on the chip to do so.
Abstract:
The disclosure provides a method of modulating droplet composition using a microfluidic device. Also provided is a method of combining two or more fluids for droplet formation using a microfluidic device, and devices for practicing the subject methods.
Abstract:
Systems, methods and devices are provided for the automated centrifugal processing of samples. In some embodiments, an integrated fluidic processing cartridge is provided, in which a centrifugation chamber is fluidically interfaced, through a lateral surface thereof, with a microfluidic device, and wherein the integrated fluidic processing cartridge is configured to be inserted into a centrifuge for centrifugation. A cartridge interfacing assembly may be employed to interface with the integrated fluidic processing cartridge for performing various fluidic processing steps, such as controlling the flow of fluids into and out of the centrifugation chamber, and controlling the flow of fluids into the microfluidic device, and optionally for the further fluidic processing of fluids extracted to the microfluidic device. The integrated fluidic processing cartridge may include a supernatant chamber the extraction of a supernatant thereto, and a diluent chamber for diluting a suspension collected in the centrifugation chamber.
Abstract:
The invention relates to a microfluidic device comprising an inlet port for liquid, a capillary channel in fluid connection to the inlet port for receiving liquid from the inlet port, the channel having a defined volume. At least one dissolvable valve is provided comprising a dissolvable membrane having a first side oriented towards the capillary channel, and capillary means connected to the second side of the dissolvable membrane such that when the membrane is dissolved by the liquid, liquid is transported through the valve to the second side of the membrane by capillary action. The invention relates to uses of the device, methods of fabricating the device and method of controlling a flow of liquid.
Abstract:
The present invention relates to a fluid triggerable passive valve for controlling flow of one or more fluids comprising: a volume comprising at least two capillary pressure barriers for respectively pinning a first and second fluid-fluid meniscus, the two capillary pressure barriers dividing the volume in at least three sub volumes, whereby two sub volumes being positioned upstream and one sub volume being positioned downstream with respect to the advancement direction of each respective fluid-fluid meniscus; wherein the capillary pressure barriers are spaced apart such that upon pinning of a first fluid-fluid meniscus on a first capillary pressure barrier and arrival or pinning of a second fluid-fluid meniscus on or at a second capillary pressure barrier, the two menisci touch and thereby coalesce into one fluid-fluid meniscus
Abstract:
Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.
Abstract:
The invention provides a new microfluidic device and method for performing operations on droplets. The invention extends to microfluidic systems comprising one or more of the microfluidic devices.
Abstract:
Die Erfindung betrifft eine mikrofluidische Vorrichtung, umfassend zumindest zwei übereinander angeordnete Schichten, eine Membran, welche zwischen den beiden Schichten angeordnet ist, eine Kavität in der eine der beiden Schichten und ein Kanal in der anderen der beiden Schichten, wobei die Membran zwischen Kavität und Kanal ausdehnbar angeordnet ist und wobei die Membran in zumindest ein vorgegebenes Verdrängungsvolumen ausdehnbar angeordnet ist. Die Erfindung betrifft ebenfalls ein Verfahren zur Herstellung einer mikrofluidischen Vorrichtung, eine Verwendung einer mikrofluidischen Vorrichtung sowie ein mikrofluidisches System.