公开(公告)号：WO2015059690A1

公开(公告)日：2015-04-30

申请号：PCT/IL2014/050902

申请日：2014-10-14

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： SOREK, Rotem ,  SBERRO, Hila ,  GOLDFARB, Tamara

IPC: C12N9/10 ,  C07K14/195

CPC classification number: C12N1/20 ,  A23C9/1234 ,  A23C2220/202 ,  C07K14/195 ,  C07K14/245 ,  C07K14/32 ,  C12N9/0051 ,  C12N9/1007 ,  C12N9/12 ,  C12N9/14 ,  C12N9/16 ,  C12N9/48 ,  C12N15/52 ,  C12Y108/04008 ,  C12Y201/01072 ,  C12Y207/11001 ,  C12Y301/03001 ,  C12Y304/00 ,  C12Y306/04012 ,  C12Y306/04013

Abstract: Isolated polynucleotides encoding a BREX system are provided. Accordingly there is provided an isolated polynucleotide encoding a BREX system comprising a nucleic acid sequence encoding the BREX system comprising brxC/pglY, pglZ and at least one of pglX, pglXI, brxP, brxHI, brxHH, brxL, brxD, brxA, brxB, brxF , and brxE , with the proviso that said BREX system does not comprise pglW , and wherein said BREX system confers phage resistance to a bacteria recombinantiy expressing same; Also provided is an isolated polynucleotide encoding a BREX system comprising a nucleic acid sequence encoding the BREX system comprising brxC/pglY, pglZ, pglX, pglW and at least one of brxD and brxHI , and wherein said BREX system confers phage resistance to a bacteria recombinantiy expressing same. Also provided are compositions and methods for conferring phage resistance to bacteria or for conferring bacterial susceptibility to phages.

Abstract translation: 提供编码BREX系统的分离的多核苷酸。 因此，提供了编码BREX系统的分离的多核苷酸，其包含编码BREX系统的核酸序列，其包含brxC / pglY，pglZ和pglX，pglXI，brxP，brxHI，brxHH，brxL，brxD，brxA，brxB，brxF中的至少一种 和brxE，条件是所述BREX系统不包含pglW，并且其中所述BREX系统赋予对表达其的重组细菌的噬菌体抗性; 还提供了编码BREX系统的分离的多核苷酸，其包含编码包含brxC / pglY，pglZ，pglX，pglW和至少一种brxD和brxHI的BREX系统的核酸序列，并且其中所述BREX系统赋予对重组细菌的噬菌体抗性 表达相同。 还提供了赋予细菌噬菌体抗性或赋予细菌对噬菌体易感性的组合物和方法。

公开(公告)号：WO2018142416A1

公开(公告)日：2018-08-09

申请号：PCT/IL2018/050134

申请日：2018-02-06

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： SOREK, Rotem ,  SBERRO, Hila ,  OFIR, Gal ,  MELAMED, Sarah

IPC: C07K14/195 ,  C12N9/10 ,  C12N1/20

CPC classification number: C12Y306/04013 ,  A61P31/04 ,  C07K14/195 ,  C07K14/32 ,  C12N1/20 ,  C12N9/1007 ,  C12N15/52 ,  C12Y201/01037 ,  C12Y306/04012

Abstract: Isolated cells genetically modified to express a DISRAM system are provided. Accordingly there is provided an isolated cell genetically modified to express a DISARM system having an anti-phage activity, the system comprising a drmM polypeptide; a drmA polypeptide; a drmB polypeptide; and a drmD polypeptide or a drmE polypeptide. Also provided are compositions and methods for conferring phage resistance to bacteria or for conferring bacterial susceptibility to phages.