公开(公告)号：WO2015059690A8

公开(公告)日：2015-07-23

申请号：PCT/IL2014050902

申请日：2014-10-14

Applicant: YEDA RES & DEV

Inventor： SOREK ROTEM ,  SBERRO HILA ,  GOLDFARB TAMARA

IPC: C12N9/10 ,  C07K14/195

CPC classification number: C12N1/20 ,  A23C9/1234 ,  A23C2220/202 ,  C07K14/195 ,  C07K14/245 ,  C07K14/32 ,  C12N9/0051 ,  C12N9/1007 ,  C12N9/12 ,  C12N9/14 ,  C12N9/16 ,  C12N9/48 ,  C12N15/52 ,  C12Y108/04008 ,  C12Y201/01072 ,  C12Y207/11001 ,  C12Y301/03001 ,  C12Y304/00 ,  C12Y306/04012 ,  C12Y306/04013

Abstract: Isolated polynucleotides encoding a BREX system are provided. Accordingly there is provided an isolated polynucleotide encoding a BREX system comprising a nucleic acid sequence encoding the BREX system comprising brxC/pglY, pglZ and at least one of pglX, pglXI, brxP, brxHI, brxHII, brxL, brxD, brxA, brxB, brxF, and brxE, with the proviso that said BREX system does not comprise pglW, and wherein said BREX system confers phage resistance to a bacteria recombinantly expressing same; Also provided is an isolated polynucleotide encoding a BREX system comprising a nucleic acid sequence encoding the BREX system comprising brxC/pglY, pglZ, pglX, pglW and at least one of brxD and brxHI, and wherein said BREX system confers phage resistance to a bacteria recombinantly expressing same. Also provided are compositions and methods for conferring phage resistance to bacteria or for conferring bacterial susceptibility to phages.

Abstract translation: 提供编码BREX系统的分离的多核苷酸。 因此，提供了编码BREX系统的分离的多核苷酸，其包含编码包含brxC / pglY，pglZ和pglX，pglXI，brxP，brxHI，brxHII，brxL，brxD，brxA，brxB，brxD，brxA，brxB，brxF中的至少一种的BREX系统的核酸序列 和brxE，条件是所述BREX系统不包含pglW，并且其中所述BREX系统赋予对重组表达的细菌的噬菌体抗性; 还提供了编码BREX系统的分离的多核苷酸，其包含编码包含brxC / pglY，pglZ，pglX，pglW和至少一种brxD和brxHI的BREX系统的核酸序列，并且其中所述BREX系统将重组的噬菌体抗性赋予细菌 表达相同。 还提供了赋予细菌噬菌体抗性或赋予细菌对噬菌体易感性的组合物和方法。

公开(公告)号：WO2015001467A1

公开(公告)日：2015-01-08

申请号：PCT/IB2014/062724

申请日：2014-06-30

Applicant: INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE

Inventor： MERCIER, Raphaël ,  GIRARD, Chloé ,  CRISMANI, Wayne

IPC: A01H5/00 ,  C07K14/415 ,  C12N15/82

CPC classification number: C12N15/8241 ,  A01H5/00 ,  C07K14/415 ,  C12N9/14 ,  C12N15/8218 ,  C12Y306/01003 ,  C12Y306/04012

Abstract: L'invention est relative à une méthode pour augmenter la fréquence de recombinaison méiotique chez les plantes, par l'inactivation de la protéine FIDG, notamment par mutagénèse ou extinction du gène FIDG codant pour ladite protéine. La présente invention est utilisable notamment dans le domaine de l'amélioration des plantes et de la cartographie génétique.

Abstract translation: 本发明涉及通过使FIDG蛋白失活，特别是通过诱变或沉默编码所述蛋白质的FIDG基因来增加植物中减数分裂重组的频率的方法。 本发明特别可用于植物改良和遗传作图领域。

公开(公告)号：WO2018142416A1

公开(公告)日：2018-08-09

申请号：PCT/IL2018/050134

申请日：2018-02-06

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： SOREK, Rotem ,  SBERRO, Hila ,  OFIR, Gal ,  MELAMED, Sarah

IPC: C07K14/195 ,  C12N9/10 ,  C12N1/20

CPC classification number: C12Y306/04013 ,  A61P31/04 ,  C07K14/195 ,  C07K14/32 ,  C12N1/20 ,  C12N9/1007 ,  C12N15/52 ,  C12Y201/01037 ,  C12Y306/04012

Abstract: Isolated cells genetically modified to express a DISRAM system are provided. Accordingly there is provided an isolated cell genetically modified to express a DISARM system having an anti-phage activity, the system comprising a drmM polypeptide; a drmA polypeptide; a drmB polypeptide; and a drmD polypeptide or a drmE polypeptide. Also provided are compositions and methods for conferring phage resistance to bacteria or for conferring bacterial susceptibility to phages.

公开(公告)号：WO2017117331A1

公开(公告)日：2017-07-06

申请号：PCT/US2016/069078

申请日：2016-12-29

Applicant: THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  MAEDA, Takahiro

Inventor： MAEDA, Takahiro

IPC: G01N33/50 ,  A61K38/00 ,  C07K14/47 ,  C12N9/90 ,  G01N33/68

CPC classification number: G01N33/6872 ,  A61K38/46 ,  A61K39/395 ,  C07K14/4702 ,  C07K2319/00 ,  C07K2319/10 ,  C07K2319/81 ,  C12N9/90 ,  C12Y306/04012 ,  G01N33/5008 ,  G01N2500/02

Abstract: The invention relates to method of treating or inhibiting progression of hemoglobinopathy in a subject in need thereof comprising inhibiting interaction between LRF-BTB and CHD protein-LBD.

Abstract translation: 本发明涉及治疗或抑制有此需要的受试者中血红蛋白病进展的方法，包括抑制LRF-BTB和CHD蛋白-LBD之间的相互作用。

公开(公告)号：WO2016059436A1

公开(公告)日：2016-04-21

申请号：PCT/GB2015/053097

申请日：2015-10-19

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： GARALDE, Daniel Ryan ,  HERON, Andrew John ,  JAYASINGHE, Lakmal ,  TURNER, Daniel John ,  WHITE, James

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12N9/14 ,  C12Y306/04012 ,  G01N27/44791 ,  C12Q2521/513 ,  C12Q2525/121 ,  C12Q2525/197 ,  C12Q2565/631

Abstract: The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNApolynucleotidehas been modified to increase DNA helicase binding thereto.

Abstract translation: 本发明涉及通过在靶RNA多核苷酸相对于跨膜孔移动时进行一个或多个测量来表征靶RNA多核苷酸的新方法。 运动由DNA解旋酶控制。 本发明还涉及修饰的RNA构建体，其中已经修饰了RNA多核苷酸以增加与其结合的DNA解旋酶。

公开(公告)号：WO2016055777A2

公开(公告)日：2016-04-14

申请号：PCT/GB2015/052916

申请日：2015-10-06

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： HERON, Andrew ,  BOWEN, Rebecca Victoria ,  BRUCE, Mark ,  JAYASINGHE, Lakmal ,  LLOYD, Joseph Hargreaves ,  SOEROES, Szabolcs ,  WALLACE, Elizabeth Jayne ,  YOUD, Christopher Peter

IPC: C07K14/35

CPC classification number: C12N9/14 ,  C12Q1/68 ,  C12Q1/6869 ,  C12Y306/04012 ,  C12Q2521/513 ,  C12Q2565/631

Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Abstract translation: 本发明涉及经修饰的Dda解旋酶，其可用于控制多核苷酸的移动，并且特别适用于测序多核苷酸。

公开(公告)号：WO2014013260A1

公开(公告)日：2014-01-23

申请号：PCT/GB2013/051925

申请日：2013-07-18

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： HERON, Andrew ,  CLARKE, James ,  MOYSEY, Ruth ,  WALLACE, Jayne ,  BRUCE, Mark ,  JAYASINGHE, Lakmal ,  CAPROTTI, Domenico ,  SOROS, Szabolcs ,  MCNEILL, Luke ,  ALVES, David ,  BOWEN, Rebecca ,  MILTON, John

IPC: C12N9/14 ,  C12N9/12

CPC classification number: C12N9/14 ,  C07K2319/80 ,  C12N9/90 ,  C12Q1/6827 ,  C12Y306/04012 ,  C12Y599/01002 ,  C12Y599/01003

Abstract: The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Abstract translation: 本发明涉及具有减少的多核苷酸解偶联的修饰的解旋酶。 解旋酶可用于控制多核苷酸的运动，并且特别可用于测序多核苷酸。

公开(公告)号：WO2015150786A1

公开(公告)日：2015-10-08

申请号：PCT/GB2015/050991

申请日：2015-03-31

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： CRAWFORD, Marion Louise ,  WHITE, James

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q2565/631 ,  C12Y306/04012 ,  C12Q2525/191 ,  C12Q2563/157

Abstract: The invention relates to a method for method of characterising, such as sequencing, a target double stranded polynucleotide. The polynucleotide is coupled to a membrane using at least two adaptors with different strengths of coupling to the membrane.

Abstract translation: 本发明涉及用于表征目标双链多核苷酸的方法，例如测序。 多核苷酸使用至少两个具有不同强度的耦合至膜的衔接器与膜偶联。

公开(公告)号：WO2015059690A1

公开(公告)日：2015-04-30

申请号：PCT/IL2014/050902

申请日：2014-10-14

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： SOREK, Rotem ,  SBERRO, Hila ,  GOLDFARB, Tamara

IPC: C12N9/10 ,  C07K14/195

CPC classification number: C12N1/20 ,  A23C9/1234 ,  A23C2220/202 ,  C07K14/195 ,  C07K14/245 ,  C07K14/32 ,  C12N9/0051 ,  C12N9/1007 ,  C12N9/12 ,  C12N9/14 ,  C12N9/16 ,  C12N9/48 ,  C12N15/52 ,  C12Y108/04008 ,  C12Y201/01072 ,  C12Y207/11001 ,  C12Y301/03001 ,  C12Y304/00 ,  C12Y306/04012 ,  C12Y306/04013

Abstract: Isolated polynucleotides encoding a BREX system are provided. Accordingly there is provided an isolated polynucleotide encoding a BREX system comprising a nucleic acid sequence encoding the BREX system comprising brxC/pglY, pglZ and at least one of pglX, pglXI, brxP, brxHI, brxHH, brxL, brxD, brxA, brxB, brxF , and brxE , with the proviso that said BREX system does not comprise pglW , and wherein said BREX system confers phage resistance to a bacteria recombinantiy expressing same; Also provided is an isolated polynucleotide encoding a BREX system comprising a nucleic acid sequence encoding the BREX system comprising brxC/pglY, pglZ, pglX, pglW and at least one of brxD and brxHI , and wherein said BREX system confers phage resistance to a bacteria recombinantiy expressing same. Also provided are compositions and methods for conferring phage resistance to bacteria or for conferring bacterial susceptibility to phages.

Abstract translation: 提供编码BREX系统的分离的多核苷酸。 因此，提供了编码BREX系统的分离的多核苷酸，其包含编码BREX系统的核酸序列，其包含brxC / pglY，pglZ和pglX，pglXI，brxP，brxHI，brxHH，brxL，brxD，brxA，brxB，brxF中的至少一种 和brxE，条件是所述BREX系统不包含pglW，并且其中所述BREX系统赋予对表达其的重组细菌的噬菌体抗性; 还提供了编码BREX系统的分离的多核苷酸，其包含编码包含brxC / pglY，pglZ，pglX，pglW和至少一种brxD和brxHI的BREX系统的核酸序列，并且其中所述BREX系统赋予对重组细菌的噬菌体抗性 表达相同。 还提供了赋予细菌噬菌体抗性或赋予细菌对噬菌体易感性的组合物和方法。

公开(公告)号：WO2014135838A1

公开(公告)日：2014-09-12

申请号：PCT/GB2014/050175

申请日：2014-01-22

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： HERON, Andrew John ,  ALVES, David Antoni ,  CLARKE, James ,  CRAWFORD, Marion Louise ,  GARALDE, Daniel Ryan ,  HALL, Graham ,  TURNER, Daniel John ,  WHITE, James

IPC: C12Q1/68 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  C12N9/14 ,  C12N13/00 ,  C12Q1/6827 ,  C12Y306/04012 ,  C12Q2521/513 ,  C12Q2565/631 ,  C12Q2537/164

Abstract: The invention relates to new methods of moving helicases past spacers on polynucleotides and controlling the loading of helicases on polynucleotides. The invention also relates to new methods of characterising target polynucleotides using helicases.

Abstract translation: 本发明涉及使解旋酶在多核苷酸上移动间隔物并控制解旋酶在多核苷酸上的负载的新方法。 本发明还涉及使用解旋酶表征靶多核苷酸的新方法。