公开(公告)号：WO2019086609A1

公开(公告)日：2019-05-09

申请号：PCT/EP2018/080016

申请日：2018-11-02

申请人： PHILIP MORRIS PRODUCTS S.A

发明人： BOVET, Lucien

IPC分类号： C12N15/82

CPC分类号： C12N15/8243 ,  C12N15/8213 ,  C12N15/8218

摘要： A plant cell comprising at least one modification which modulates the expression or activity of polynucleotides or polypeptides homologous to SEQ ID NOs: 1-4. A method for producing a plant with modulated expression or activity of polynucleotides or polypeptides homologous to SEQ ID NOs: 1-4. Plants with modulated alkaloid concentrations, and methods for their production.

公开(公告)号：WO2018192961A1

公开(公告)日：2018-10-25

申请号：PCT/EP2018/059880

申请日：2018-04-18

申请人： SACK, Markus ,  BORTESI, Luisa

发明人： SACK, Markus ,  BORTESI, Luisa ,  SRUTHY, Maria ,  FISCHER, Rainer ,  ZISCHEWSKI, Julia

IPC分类号： C12N15/82

CPC分类号： C12N15/8213

摘要： The technology provided herein relates to methods for the delivery of pre-assembled ribonucleoprotein complexes (RNP) - such as the Cas9/gRNA RNP complex - for highly efficient genome editing to intact differentiated cells and regeneration of cell lines, whole tissues or organisms thereof.

公开(公告)号：WO2018115389A1

公开(公告)日：2018-06-28

申请号：PCT/EP2017/084291

申请日：2017-12-22

申请人： KEYGENE N.V.

发明人： BUNDOCK, Paul

IPC分类号： C12N15/82

CPC分类号： C12N15/8213 ,  C12Q2521/307

摘要： The current invention relates to methods of targeted genetic alteration in plant cells, as well as to plant cells and plants thus obtained. In the method, the use of the combination of a nickase and a single-stranded oligonucleotide of the invention, provides for targeted alteration of a DNA duplex with increased efficacy.

公开(公告)号：WO2017125931A1

公开(公告)日：2017-07-27

申请号：PCT/IL2017/050078

申请日：2017-01-19

申请人： THE STATE OF ISRAEL, MINISTRY OF AGRICULTURE & RURAL DEVELOPMENT, AGRICULTURAL RESEARCH ORGANIZATION (ARO) (VOLCANI CENTER)

发明人： BARG, Rivka ,  SALTS, Yehiam ,  KLAP, Chen ,  ARAZI, Itzhak ,  YESHAYAHOU, Ester ,  BOLGER, Anthony

IPC分类号： A01H5/08 ,  A01H1/06 ,  A23L19/00

CPC分类号： A01H5/08 ,  A23L19/00 ,  C07K14/415 ,  C12N15/8213 ,  C12N15/8216 ,  C12N15/8249 ,  C12N15/8261 ,  C12N15/8287 ,  C12N15/829 ,  Y02A40/146

摘要： A Solanaceous plant exhibiting a facultative parthenocarpy is provided. The plant comprises a loss-of-function mutation in a Sl AGL6 gene and alternatively or additionally characterized by an average fruit weight/plant at least about the same as that of a non-parthenocarpic tomato of the same genetic background under fertilization permissive conditions of the non-parthenocarpic tomato. Also provided are methods of producing such plants and processed products produced from same.

摘要翻译： 提供了表现出兼性单性结实的茄科植物。 该植物在S1 AGL6基因中包含功能缺失型突变，并且可选地或另外地以平均果实重量/植物为特征，所述平均果重/植物至少与在相同遗传背景下的非单性结实番茄的平均果重/植物在受精允许条件 非单性结实番茄。 还提供了生产这种植物和由其生产的加工产品的方法。

公开(公告)号：WO2017096237A1

公开(公告)日：2017-06-08

申请号：PCT/US2016/064733

申请日：2016-12-02

申请人： CERES, INC.

发明人： PENNELL, Roger, I. ,  HAMILTON, Richard ,  LIANG, Delin

IPC分类号： A01H1/00 ,  A01H1/04 ,  C07K14/415 ,  C12N15/82

CPC分类号： A01H1/04 ,  C12N15/8213

摘要： Described are methods and materials for the genetic modification of plants by specific gene targeting and precise editing of nucleic acid sequences in a plant. The methods and materials provided herein enable one to edit the plant genome by design to control the expression of endogenous genes and/or control the transmission and expression of transgenic traits. Provided are also methods of producing plants having a desirable agronomic trait by crossing a transgenic plant expressing a gRNA with a plant expressing a Cas enzyme, and selecting a progeny plant having the desirable agronomic trait or a seed thereof.

摘要翻译： 描述了通过植物中特定基因靶向和精确编辑核酸序列对植物进行遗传修饰的方法和材料。 本文提供的方法和材料使得能够通过设计来编辑植物基因组以控制内源基因的表达和/或控制转基因性状的传递和表达。 还提供了通过将表达gRNA的转基因植物与表达Cas酶的植物杂交并选择具有所需农艺性状或其种子的后代植物来生产具有期望农学性状的植物的方法。

公开(公告)号：WO2017079026A1

公开(公告)日：2017-05-11

申请号：PCT/US2016/059093

申请日：2016-10-27

申请人： E. I. DU PONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL, INC.

发明人： CHAKY, Julian Marco ,  CIGAN, Andrew Mark ,  KINNEY, Anthony J. ,  LIU, Zhan-Bin ,  WOODWARD, John Bryan ,  YOUNG, Joshua K.

IPC分类号： C12N15/82

CPC分类号： C12N15/8213

摘要： Compositions and methods are provided producing a complex trait locus in a genomic window of a soybean plant comprising (i) at least one transgenic target site for site specific integration integrated in at least double-strand-break target site, (ii) at least one double-strand-break target site and at least one transgene, (iii) at least one altered double-strand-break target site or (iv) any one combination of (i)-(iii). The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.

摘要翻译： 提供了在大豆植物的基因组窗口中产生复合性状基因座的组合物和方法，其包含（i）至少一个用于位点特异性整合的转基因靶位点，其整合于至少双链断裂靶标 （ii）至少一个双链断裂靶位点和至少一个转基因，（iii）至少一个改变的双链断裂靶位点或（iv）（i） - （iii）的任何一种组合， 。 双链断裂靶位点可以是但不限于锌指内切核酸酶，工程内切核酸酶，大范围核酸酶，TALEN和/或Cas核酸内切酶的靶位点。 所述植物的基因组窗口可以包含至少一个感兴趣的基因组基因座，例如性状盒，转基因，突变的基因，天然基因，编辑的基因或位点特异性整合（SSI）靶位点。

公开(公告)号：WO2017061805A1

公开(公告)日：2017-04-13

申请号：PCT/KR2016/011216

申请日：2016-10-06

申请人： 기초과학연구원 ,  서울대학교산학협력단 ,  재단법인차세대융합기술연구원

发明人： 김진수 ,  김정은 ,  최성화 ,  우제욱 ,  권순일 ,  김혜란

IPC分类号： C12N15/82 ,  C12N9/22 ,  A01H5/12

CPC分类号： A01H5/12 ,  C12N15/8213 ,  C12N15/8298

摘要： 본 발명은 Cas 단백질-가이드 RNA RNP (ribonucleoprotein)를 이용하여 식물 원형질체로부터 재생된 유전체가 교정된 식물체의 제조 효율을 증가시키는 방법에 관한 것이다. 본 발명의 유전체 교정 식물체의 제조 효율을 증가시키는 방법을 통해 표적 유전자가 변이된 식물체를 효율적으로 생산할 수 있을 뿐 아니라, 식물체 내 외래 DNA의 삽입을 최소화할 수 있다. 따라서 본 발명은 농업, 식품 및 생명공학분야 등 다양한 분야에서 매우 유용하게 사용될 수 있다.

摘要翻译： 本发明涉及通过使用Cas蛋白引导RNA核糖核蛋白（RNP）来提高从植物原生质体再生的基因组修饰植物的生产效率的方法。 提高本发明的基因组修饰植物的生产效率的方法可以有效地产生具有突变的靶基因的植物，并且还可以使外来DNA插入植物中最小化。 因此，本发明可以非常有用地用于诸如农业，食品和生物技术领域的各个领域。

公开(公告)号：WO2017028768A1

公开(公告)日：2017-02-23

申请号：PCT/CN2016/095307

申请日：2016-08-15

申请人： INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES

发明人： GAO, Caixia ,  LI, Jun

IPC分类号： C12N15/82 ,  C12N9/10 ,  C12N15/54 ,  C12N5/10 ,  A01H4/00 ,  A01H5/00

CPC分类号： C12N15/8213 ,  C12N9/1092 ,  C12N15/8275

摘要： A method for obtaining glyphosate-resistant rice by a site-directed nucleotide substitution is provided, which comprises the following steps: only substituting threonine (T) at position 8 of the amino acid sequence of a conserved region of endogenous EPSPS protein of a target plant with isoleucine (I), and substituting proline (P) at position 12 with serine (S) to obtain a plant， i.e., a glyphosate-resistant plant. A method capable of generating a site-directed nucleotide substitution and a fragment substitution utilizing a CRISPR-mediated NHEJ pathway to substitute a region between two gRNA sites by designing the two gRNA sites is provided.

摘要翻译： 提供了通过定点核苷酸取代获得抗草甘膦的方法，其包括以下步骤：仅在靶植物的内源性EPSPS蛋白质保守区的氨基酸序列的8位替代苏氨酸（T） 用异亮氨酸（I）和用丝氨酸（S）在位置12处取代脯氨酸（P）获得植物，即抗草甘膦植物。 提供了一种能够通过设计两个gRNA位点产生利用CRISPR介导的NHEJ途径替代两个gRNA位点之间的区域的定点核苷酸取代和片段取代的方法。

公开(公告)号：WO2016116032A1

公开(公告)日：2016-07-28

申请号：PCT/CN2016/071352

申请日：2016-01-19

申请人： INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY,CHINESE ACADEMY OF SCIENCES

发明人： GAO, Caixia ,  WANG, Yanpeng ,  ZHANG, Yi ,  LIU, Jinxing ,  ZHANG, Kang

IPC分类号： C12N15/82 ,  C12N15/55 ,  C12N5/10 ,  A01H5/00

CPC分类号： C12N15/8213 ,  A01H1/04 ,  A01H4/008 ,  C12N5/14

摘要： Provided is a method for conducting site-specific modification in a plant through gene transient expression, comprising the following steps: transiently expressing a sequence-specific nuclease specific to the target fragment in the cell or tissue of the plant of interest, wherein the sequence-specific nuclease is specific to the target site and the target site is cleaved by the nuclease, thereby the site-specific modification of the target site is achieved through DNA repairing of the plant.

摘要翻译： 提供了一种通过基因瞬时表达在植物中进行位点特异性修饰的方法，其包括以下步骤：瞬时表达对目标植物的细胞或组织中靶标片段特异性的序列特异性核酸酶，其中序列 - 特异性核酸酶对靶位点特异性，靶位点被核酸酶切割，从而通过植物的DNA修复实现靶位点的位点特异性修饰。

公开(公告)号：WO2016084084A1

公开(公告)日：2016-06-02

申请号：PCT/IL2015/051150

申请日：2015-11-26

申请人： DANZIGER INNOVATIONS LTD.

发明人： VAINSTEIN, Alexander ,  MARTON, Ira ,  HONIG, Arik ,  MARHEVKA, Elena

IPC分类号： C12N15/83 ,  C12N5/04 ,  A01H5/00

CPC分类号： C12N15/8213 ,  C12N15/8203 ,  C12N15/8216 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8279

摘要： A nucleic acid construct is provided. The construct comprises a tobacco rattle virus (TRV) sequence and a nucleic acid sequence encoding a single guide RNA (sgRNA) that mediates sequence-specific cleavage in a target sequence of a genome of interest, wherein the TRV sequence is devoid of a functional 2b sequence. Also provided are plant cells comprising the construct and uses of the construct in gene editing.

摘要翻译： 提供核酸构建体。 该构建体包括烟草拨浪鼠病毒（TRV）序列和编码单指导RNA（sgRNA）的核酸序列，该单引导RNA（sgRNA）介导目标基因组的靶序列中的序列特异性切割，其中TRV序列没有功能性2b 序列。 还提供了包含构建体和基因编辑中构建体的用途的植物细胞。