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    METHOD AND KIT OF DETECTING THE ABSENCE OF MICRO-ORGANISMS
    1.
    发明申请
    METHOD AND KIT OF DETECTING THE ABSENCE OF MICRO-ORGANISMS 审中-公开
    检测微量有机物的方法和试剂盒

    公开(公告)号：WO2016005768A1

    公开(公告)日：2016-01-14

    申请号：PCT/GB2015/052006

    申请日：2015-07-10

    Applicant: MOMENTUM BIOSCIENCE LIMITED

    Inventor： CROW, Matthew Alun ,  BENNETT, Helen Victoria ,  WRATTING, Daniel Stephen ,  MULLEN, William Henry

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q1/04 ,  C12Q1/6848 ,  C12Q2521/101 ,  C12Q2525/101 ,  C12Q2525/113 ,  G01N2333/91245

    Abstract: Methods of detecting the absence or presence of a micro-organism in a sample comprising: contacting the sample with a nucleic acid molecule which acts as a substrate for nucleic acid modifying activity of the micro-organism in the sample, incubating the thus contacted sample under conditions suitable for nucleic acid modifying activity; and specifically determining the absence or presence of a modified nucleic acid molecule resulting from the action of the nucleic acid modifying activity on the substrate nucleic acid molecule to indicate the absence or presence of the micro-organism. Corresponding kits are also provided.

    Abstract translation: 检测样品中微生物不存在或不存在的方法，其包括：将样品与作为底物的核酸分子接触，用于样品中微生物的核酸修饰活性，将如此接触的样品孵育在 适合于核酸修饰活性的条件; 并且具体地确定由所述底物核酸分子上的核酸修饰活性的作用产生的修饰的核酸分子的存在或不存在，以指示微生物的存在或不存在。 还提供了相应的工具包。

    TEST SYSTEM FOR DETERMINING THE ACTIVITY OF CYCLO-NUCLEOTIDE-DEPENDENT PROTEIN KINASES AND VASP PHOSPHATASES
    6.
    发明申请
    TEST SYSTEM FOR DETERMINING THE ACTIVITY OF CYCLO-NUCLEOTIDE-DEPENDENT PROTEIN KINASES AND VASP PHOSPHATASES 审中-公开
    测试系统用于确定CYCLO核苷酸依赖性蛋白激酶和磷酸酶的VASP ACTIVITY

    公开(公告)号：WO0196594A3

    公开(公告)日：2002-05-16

    申请号：PCT/EP0106621

    申请日：2001-06-12

    Applicant: VASOPHARM BIOTECH GMBH ,  DRUECKES PETER ,  JARCHAU THOMAS ,  WALTER ULRICH ,  BADER BENJAMIN

    Inventor： DRUECKES PETER ,  JARCHAU THOMAS ,  WALTER ULRICH ,  BADER BENJAMIN

    IPC: C12Q1/48 ,  C12Q1/42 ,  G01N33/573

    CPC classification number: C12Q1/485 ,  G01N2333/91245

    Abstract: The invention relates to an HTS-appropriate test system for detecting the activity of cyclo-nucleotide-dependent protein kinases (cNPK) containing: a) at least one test compound; b) at least one appropriate cNPK substrate; c) at least one composition, which is to be incubated and which contains cNPK and ATP, optionally, phosphorylation reaction stoppers, and; d) an appropriate detection system for quantifying the phosphorylation of the cNPK substrate. The invention also relates to an HTS-appropriate test system for detecting the activity of VASP phosphatases containing: e) at least one test compound; f) at least one appropriate VASP phosphatase substrate; g) at least one composition, which is to be incubated and which contains VASP phosphatase, and; h) an appropriate detection system for quantifying the dephosphorylation of the VASP phosphatase substrate. The described test systems can be used for locating compounds, which modulate the activity of a cNPK or of a VASP phosphatase, from chemical or natural substance libraries.

    Abstract translation: 公开的是用于检测的环核苷酸依赖的蛋白激酶的（cNPK）的活性的HTS合适的测试系​​统，其包含a）至少一种测试化合物，b）至少一种合适的cNPK衬底，c）至少一个被温育组合物，包含cNPK和ATP，如果必要的话 。Phosphorylierungsreaktionsstopper，以及d）用于cNPK底物磷酸化的定量的合适的检测系统。 此外，HTS合适的测试系​​统是用于检测VASP磷酸酶的描述包含E）的活性的至少一种测试化合物，f）至少一种合适的VASP磷酸酶底物，g）至少一个被温育组合物含有VASP磷酸酶，以及h）一 为VASP磷酸酶底物的去磷酸化的定量合适的检测系统。 描述的测试系统可以找到调制cNPK或VASP磷酸酶，使用化学或天然物质文库的活性的化合物。

    IMPROVED DNA POLYMERASE ACTIVITY ASSAYS AND METHODS ENABLING DETECTION OF VIABLE MICROBES
    7.
    发明申请
    IMPROVED DNA POLYMERASE ACTIVITY ASSAYS AND METHODS ENABLING DETECTION OF VIABLE MICROBES 审中-公开
    改进的DNA聚合酶活性测定和方法启用检测可变微生物

    公开(公告)号：WO2013103744A1

    公开(公告)日：2013-07-11

    申请号：PCT/US2013/020180

    申请日：2013-01-03

    Applicant: ZEUS SCIENTIFIC, INC.

    Inventor： O'HARA, Shawn, Mark ,  SWEITZIG, Daniel

    IPC: C12Q1/68 ,  C12Q1/70 ,  G01N33/50 ,  G01N33/53

    CPC classification number: C12Q1/686 ,  C12Q1/04 ,  C12Q1/48 ,  C12Q1/689 ,  G01N2333/91245 ,  C12Q2521/101

    Abstract: A method for performing a diagnostic assay for the detection of the presence or amount of a microorganism within a sample matrix containing active DNA polymerase, is disclosed. The method utilizes the measurement of DNA polymerase extension activity, wherein the assay comprises the steps of incubating DNA polymerase in the sample matrix with a selected suitable substrate, and performing PCR cycling and detection via the use of a selected suitable nucleic acid probe, thereby to detect endogenous DNA polymerase extension activity in the sample matrix as an indication of the presence or amount of said microorganism.

    Abstract translation: 公开了一种用于进行用于检测含有活性DNA聚合酶的样品基质中微生物的存在或量的诊断测定的方法。 该方法利用DNA聚合酶延伸活性的测定，其中测定法包括以下步骤：将样品基质中的DNA聚合酶与选定的合适底物一起孵育，并通过使用选择的合适的核酸探针进行PCR循环和检测，从而 检测样品基质中的内源DNA聚合酶延伸活性作为所述微生物的存在或量的指示。

    ASSAY FOR TELOMERASE ACTIVITY
    8.
    发明申请
    ASSAY FOR TELOMERASE ACTIVITY 审中-公开
    测定TELOMERASE活性

    公开(公告)号：WO2011106671A1

    公开(公告)日：2011-09-01

    申请号：PCT/US2011/026281

    申请日：2011-02-25

    Applicant: GERON CORPORATION ,  PIROT, Zhu, Zhen

    Inventor： PIROT, Zhu, Zhen

    IPC: C12Q1/68

    CPC classification number: C12Q1/48 ,  G01N2333/91245

    Abstract: The invention is directed to methods for determining the level of telomerase reverse transcriptase activity in mammalian cells.

    Abstract translation: 本发明涉及确定哺乳动物细胞中端粒酶逆转录酶活性水平的方法。

    SCREEN FOR ANTI-VIRAL COMPOUNDS AGAINST HEPATITIS B VIRUS
    10.
    发明申请
    SCREEN FOR ANTI-VIRAL COMPOUNDS AGAINST HEPATITIS B VIRUS 审中-公开
    用于抗乙型肝炎病毒的抗病毒化合物的筛选

    公开(公告)号：WO2010037083A1

    公开(公告)日：2010-04-01

    申请号：PCT/US2009/058727

    申请日：2009-09-29

    Applicant: SAINT LOUIS UNIVERSITY ,  TAVIS, John, E. ,  BADTKE, Matthew, P.

    Inventor： TAVIS, John, E. ,  BADTKE, Matthew, P.

    IPC: G01N33/48

    CPC classification number: G01N33/569 ,  G01N2333/02 ,  G01N2333/91245 ,  G01N2500/04

    Abstract: Methods of identifying compounds having anti-viral activity against Hepatitis B Virus (HBV) and other hepadnavirυses are disclosed. In these methods, a mixture is formed comprising a candidate compound, a T3 domain of a hepadnavirus polymerase and/or an RT-1 domain of a hepadnavirus polymerase, and a nucleobase polymer. When more than one polymerase domain is used, the domains can be from the same species of hepadnavirus, such as Duck Hepatitis B Virus (DHBV), or from different species of hepadnavirus. Compounds having anti-viral activity against HBV are identified as compounds which interfere with the binding between T3 domain and a nucleobase polymer, or between an RT-1 domain and a nucleobase polymer.

    Abstract translation: 公开了鉴定具有针对乙型肝炎病毒（HBV）和其他肝素病毒的抗病毒活性的化合物的方法。 在这些方法中，形成包含候选化合物，肝炎链球菌病毒聚合酶的T3结构域和/或肝炎链球菌病毒聚合酶的RT-1结构域和核碱基聚合物的混合物。 当使用多于一个聚合酶结构域时，结构域可以来自相同种类的肝炎病毒，例如鸭乙型肝炎病毒（DHBV）或来自不同种类的肝炎病毒。 具有抗HBV的抗病毒活性的化合物被鉴定为干扰T3结构域与核碱基聚合物之间或RT-1结构域与核碱基聚合物之间结合的化合物。

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