摘要:
Microscope system for, and methods of, imaging a sample including biological cells. In an exemplary method, light transmitted through the sample may be detected for a first set of focal positions to collect a first stack of images. Values of a focus metric may be calculated for the first stack of images. A candidate focal position may be determined based on the values. Photoluminescence may be detected from the sample for a second set of focal positions to collect a second stack of images. The second set of focal positions may define a smaller range than the first set of focal positions. At least one focal position of the second set of focal positions may be based on the candidate focal position. In other words, the candidate focal position may serve as a guide for finding a suitable photoluminescence focal position.
摘要:
一种基于可控制镜头倾斜的摄像装置的三维快速自动对焦方法,包括下述步骤:(1)、选择N个对焦点,N≥2;(2)、镜头沿z轴方向前后移动一遍后获得相应的数据:A'(x A ,y A ),B'(x B ,y B )......N'(x N ,y N )以及f A ,f B ......f N ,其中N'(x N ,y N )为对焦点N在影像传感器上成像的位置坐标,f N 为对焦点N处清晰时镜头的行程;(3)、将步骤(2)中获取到的数据代入公式kf N =-x N θ y +y N θ x +kf,建立方程组;(4)、求解步骤(3)中方程组,获得使各个设定的对焦点同时清晰成像在影像传感器上时镜头所需要的偏转角度θ x ,θ y 及前后移动的行程f;(5)、用步骤(4)中求得的该组参数值输出驱动镜头,实现各个设定的目标对焦点同时在图像传感器上获得清晰的对焦。
摘要:
Provided are an image pickup apparatus and method capable of improving a resolution of an image having depth information. The image pickup apparatus may include: a main lens configured to refract incident light; an image sensor comprising a plurality of two-dimensional (2D)-arranged pixels configured to output an image signal according to the incident light; a micro lens array between the main lens and the image sensor and comprising a plurality of 2D-arranged micro lenses; and a controller configured to receive the image signal from the image sensor and to generate an image according to the received image signal, wherein the controller is configured to obtain a plurality of images having different depths of field by changing a distance between the main lens and the image sensor and to obtain at least one depth map from the at least one of the obtained plurality of images.
摘要:
Methods, systems and computer program products relating to digital microscopy are disclosed. A digital microscopy method may comprise capturing a plurality of overlapping images of a sample, wherein the capturing of at least one of the plurality of images is performed while the sample and a focal plane are in relative motion along an optical axis at a speed greater than zero; and processing the plurality of images using a reference criterion to determine a reference relative arrangement of the focal plane and the sample along the optical axis.
摘要:
A variable focus lens comprises: a plurality of microlenses into which light falls incident; a plurality of first microprisms which are positioned respectively corresponding to the plurality of microlenses, and which cause the path of incident light from the corresponding microlens to change to a first direction, based on a predetermined first tilt angle; and a plurality of second microprisms which are positioned respectively corresponding to the plurality of first microprisms, and which cause the path of incident light from the corresponding first microprism to change to a second direction at right angles to the first direction, based on a predetermined second tilt angle. And, here, the focal points of the microlenses, the first tilt angle of the first microprisms and the second tilt angle of the second microprisms are adjusted in such a way that the light incident on the microlenses collects at any one point.
摘要:
An auto-focus system employing a tunable liquid crystal lens is provided that collects images at different optical power values as the liquid crystal molecules are excited between a ground state and a maximum optical power state tracking image focus scores. An image is acquired at a desired optical power value less than maximum optical power established with the liquid crystal molecules closer a fully excited state than the maximum optical power state having the same image focus score. This drive signal employed during image acquisition uses more power than was used to achieve the same optical power value during the auto-focus scan, while actively driving the liquid crystal molecules is fast. A pause due to image transfer/processing delays after acquisition is employed to allow slow relaxation of the liquid crystal molecules back to the ground state in preparation for a subsequent focus search.