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    METHODS OF CULTURING A MAMMALIAN CELL
    3.
    发明申请
    METHODS OF CULTURING A MAMMALIAN CELL 审中-公开
    培养哺乳动物细胞的方法

    公开(公告)号：WO2016106192A2

    公开(公告)日：2016-06-30

    申请号：PCT/US2015/067040

    申请日：2015-12-21

    Applicant: GENZYME CORPORATION

    Inventor： HWANG, Christopher ,  JOHNSON, Timothy ,  WALTHER, Jason ,  CHENG, Cheng ,  WANG, Jonathan ,  SHAH, Neha ,  BAE, Seul-A

    IPC: C12N5/00

    CPC classification number: C12P21/00 ,  C07K16/00 ,  C07K2317/14 ,  C12N5/0043 ,  C12N5/005 ,  C12N2500/50 ,  C12N2510/02

    Abstract: Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

    Abstract translation: 本文提供了在液体培养基中培养哺乳动物细胞的方法，所述液体培养基包括浓度为1.8g / L或多于1.8g / L的更高浓度的泊洛沙姆-188或液体培养基，所述液体培养基包括 基于选自以下的一种或多种因子选择的泊洛沙姆-188浓度：孔径，孔型，气体流速，培养基中活细胞密度和与细胞应激相关的标记。

    METHODS FOR IMPROVED PRODUCTION AND RECOVERY OF RECOMBINANT PROTEINS FROM EUKARYOTIC CELL CULTURES
    4.
    发明申请
    METHODS FOR IMPROVED PRODUCTION AND RECOVERY OF RECOMBINANT PROTEINS FROM EUKARYOTIC CELL CULTURES 审中-公开
    用于改善重组蛋白从原核细胞培养物生产和恢复的方法

    公开(公告)号：WO2014110440A1

    公开(公告)日：2014-07-17

    申请号：PCT/US2014/011139

    申请日：2014-01-10

    Applicant: BIOGEN IDEC MA INC.

    Inventor： GILBERT, Alan ,  YANG, William C. ,  HUANG, Yao-Ming ,  LU, Jiuyi

    IPC: C12P21/04 ,  C12N5/07 ,  C12N5/00

    CPC classification number: C12N1/38 ,  C12N5/0037 ,  C12N2500/12 ,  C12N2500/24 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/50 ,  C12N2500/60 ,  C12N2501/065 ,  C12N2501/90 ,  C12N2510/02 ,  C12P21/02

    Abstract: The present disclosure is directed to methods to improve the yields of proteins recombinantly produced in eukaryotic cells comprising the addition of anionic substances such as valproate, malate, succinate, fumarate, citrate (e.g., ferric citrate), polyanionic compounds (e.g., polysulfated compounds such dextran sulfate or polyvinyl sulfate), and combinations thereof, wherein the addition of such compounds reduces the binding of recombinant proteins to the cell surface, therefore increasing the amount of soluble protein that can recovered from the culture medium. In addition, the protein yields can be improved according to the disclosed methods by preventing or reducing cell growth inhibition caused by the binding of the expressed recombinant proteins (e.g., neublastin, or antibodies or antigen-binding fragments thereof) to the cell surface. The disclosed anionic substances can also be used during the harvesting phase of a protein production process to release the expressed recombinant proteins from the cell surface prior to protein purification.

    Abstract translation: 本公开涉及提高在真核细胞中重组产生的蛋白质的产量的方法，包括加入阴离子物质如丙戊酸盐，苹果酸盐，琥珀酸盐，富马酸盐，柠檬酸盐（例如柠檬酸铁），聚阴离子化合物（例如多硫酸化合物 硫酸葡聚糖或聚乙烯硫酸盐）及其组合，其中加入这些化合物降低重组蛋白与细胞表面的结合，从而增加可从培养基中回收的可溶性蛋白质的量。 此外，根据所公开的方法，通过预防或减少由表达的重组蛋白（例如，neburgastin，或其抗体或其抗原结合片段）与细胞表面的结合引起的细胞生长抑制，可以提高蛋白质产量。 所公开的阴离子物质还可以在蛋白质生产过程的收获阶段期间使用，以在蛋白质纯化之前从细胞表面释放所表达的重组蛋白质。

    DEVICE FOR MANUFACTURING POLYMER FIBERS AND USES THEREOF
    5.
    发明申请
    DEVICE FOR MANUFACTURING POLYMER FIBERS AND USES THEREOF 审中-公开
    用于制造聚合物纤维的装置及其用途

    公开(公告)号：WO2013137833A1

    公开(公告)日：2013-09-19

    申请号：PCT/SG2013/000110

    申请日：2013-03-18

    Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    Inventor： WAN, Andrew Chwee Aun ,  LEONG, Meng Fatt ,  LIM, Tze Chiun ,  YING, Jackie Y. ,  TOH, Jerry Kah Chin

    IPC: D01D5/38 ,  D01D5/30 ,  B29C47/00 ,  A61L33/06

    CPC classification number: D01D5/12 ,  A61L27/24 ,  A61L27/38 ,  A61L27/56 ,  C12N11/04 ,  C12N2500/34 ,  C12N2500/50 ,  D01D5/30 ,  D01D5/38

    Abstract: There is provided a device, and related method and uses, for drawing a polymer fiber, the device comprising: a. at least two polymer compartments, wherein each polymer compartment is capable of retaining a polymer solution, and wherein adjacent compartments comprise different polymer solutions; and b. a slider comprising at least one prong, wherein the prong is capable of contacting the different polymer solutions, and wherein the slider is arranged in a retractable manner from the at least two polymer compartments. There is further provided a system and a related method for manufacturing a polymer fiber.

    Abstract translation: 提供了一种用于拉伸聚合物纤维的装置及相关方法和用途，所述装置包括：a。 至少两个聚合物隔室，其中每个聚合物隔室能够保留聚合物溶液，并且其中相邻隔室包含不同的聚合物溶液; 和b。 滑块，其包括至少一个插脚，其中所述插脚能够接触所述不同的聚合物溶液，并且其中所述滑块以可缩回的方式从所述至少两个聚合物隔间布置。 还提供了一种用于制造聚合物纤维的系统和相关方法。

    STIMULATION, CULTURE AND PRESERVATION OF PANCREATIC AND OTHER CELLS
    9.
    发明申请
    STIMULATION, CULTURE AND PRESERVATION OF PANCREATIC AND OTHER CELLS 审中-公开
    刺激，培养和保存胰腺和其他细胞

    公开(公告)号：WO98041617A1

    公开(公告)日：1998-09-24

    申请号：PCT/SE1998/000475

    申请日：1998-03-16

    IPC: A01N1/02 ,  A61K9/08 ,  A61K31/717 ,  A61K35/39 ,  A61P11/00 ,  A61P11/04 ,  A61P17/02 ,  C12N5/071 ,  C12P21/02 ,  C12N5/08 ,  C07K14/62

    CPC classification number: C12N5/0676 ,  A01N1/0226 ,  C12N2500/50 ,  C12P21/02

    Abstract: A method for stimulating pancreatic cells to synthesize and/or excrete insulin comprises contacting insulin-producing cells, in particular their aqueous suspension, with a water-soluble cellulose derivative, in particular selected from alkylated, hydroxyalkylated, and alkylated-hydroxyalkylated cellulose or a mixture thereof. A medium for the culture of pancreatic beta -cells contains an effective cell-stimulating amount of a cellulose derivative. It can be used to stimulate pancreatic beta -cells to produce and/or excrete insulin. An apparatus for such stimulation comprises a container holding a solution of a cellulose derivative in an aqueous culture medium. Stimulation of pancreatic beta -cells by a cellulose derivative is useful in the management of diabetes. Further preservative or therapeutic methods using the aqueous cellulose derivatives are disclosed.

    Abstract translation: 用于刺激胰腺细胞合成和/或排泄胰岛素的方法包括使产生胰岛素的细胞，特别是其水性悬浮液与水溶性纤维素衍生物，特别是选自烷基化，羟烷基化和烷基化羟烷基化的纤维素或混合物 它们。 用于胰β细胞培养的培养基含有有效的细胞刺激量的纤维素衍生物。 它可用于刺激胰腺β-细胞产生和/或排泄胰岛素。 用于这种刺激的装置包括容纳纤维素衍生物在水性培养基中的溶液的容器。 通过纤维素衍生物刺激胰腺β-细胞可用于糖尿病的治疗。 公开了使用水性纤维素衍生物的其它防腐剂或治疗方法。

    LIPID MICROEMULSIONS FOR CULTURE MEDIA
    10.
    发明申请
    LIPID MICROEMULSIONS FOR CULTURE MEDIA 审中-公开
    文化媒体的脂质微乳液

    公开(公告)号：WO1989001027A1

    公开(公告)日：1989-02-09

    申请号：PCT/US1988002440

    申请日：1988-07-20

    Applicant: CETUS CORPORATION

    Inventor： CETUS CORPORATION ,  INLOW, Duane

    IPC: C12N05/00

    CPC classification number: C12N15/86 ,  C07K14/53 ,  C12N5/0037 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/50 ,  C12N2710/14143

    Abstract: Lipid microemulsions which can be added to cell culture media to provide essential lipids in a bioavailable form and their components are disclosed. Methods to disperse lipids in culture media by the use of one or more emulsifiers are described. Further disclosed are media which support growth of cells and production of recombinant, viral and/or native products wherein lipids are supplied in the form of a microemulsion.

    Abstract translation: 公开了可以添加到细胞培养基中以提供生物可利用形式的基本脂质及其组分的脂质微乳液。 描述了通过使用一种或多种乳化剂将脂质分散在培养基中的方法。 进一步公开的是支持细胞生长和生产重组，病毒和/或天然产物的培养基，其中脂质以微乳液的形式提供。

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