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    NUCLEOTIDES COMPRISING CASPHI MODIFIED FOR NUCLEAR TARGETING
    1.
    发明申请
    NUCLEOTIDES COMPRISING CASPHI MODIFIED FOR NUCLEAR TARGETING 审中-公开

    公开(公告)号：WO2023028254A2

    公开(公告)日：2023-03-02

    申请号：PCT/US2022/041561

    申请日：2022-08-25

    申请人： VANDERBILT UNIVERSITY

    发明人： REX, Tonia S. ,  BACKSTROM, Jon R.

    IPC分类号： C12N15/09 ,  C12N15/11 ,  C12N15/63 ,  C12N15/79 ,  C12N15/85 ,  C12N15/86 ,  C12N15/87 ,  C12N15/90 ,  A61K48/00 ,  C12N15/00 ,  C12N15/102 ,  C12N15/635 ,  C12N2310/20 ,  C12N2750/14143

    摘要： The presently-disclosed subject matter relates to a compositions and methods that makes use of a CasΦ(CasPhi) that has been modified for effective nuclear targeting. In particular, certain embodiments of the presently-disclosed subject matter relate to unique nucleic acid molecules, compositions, and methods for delivery of CasPhi to the nucleus to effectively modulate expression or otherwise target of a gene of interest. A nucleic acid disclosed herein includes a nucleotide encoding a CasPhi, wherein there are no amino acid substitutions, or wherein proline residues at amino acids 749 and 753 have been substituted; a nucleotide encoding an amino-terminal linker connected to the amino-terminal end of the CasPhi; a nucleotide encoding a carboxy -terminal linker connected to the carboxy-terminal end of the CasPhi; and a nucleotide encoding a nuclear localization signal (NLS) downstream from the CasPhi and carboxy-terminal linker.

    COMPOSITIONS AND METHODS FOR CONTROLLING ADAPTIVE IMMUNITY IN BACTERIA
    2.
    发明申请
    COMPOSITIONS AND METHODS FOR CONTROLLING ADAPTIVE IMMUNITY IN BACTERIA 审中-公开

    公开(公告)号：WO2023016978A1

    公开(公告)日：2023-02-16

    申请号：PCT/EP2022/072214

    申请日：2022-08-08

    申请人： DUPONT NUTRITION BIOSCIENCES APS

    发明人： MILLEN, Anne M. ,  ROMERO, Dennis ,  SIMDON, Laura

    IPC分类号： C07K14/195 ,  C12N15/74 ,  C12N15/10 ,  C12N15/102 ,  C12N15/746 ,  C12N2310/20 ,  C12N9/22 ,  C12R2001/46

    摘要： Provided herein are compositions for enabling natural adaptive spacer acquisition in CRISPR-Cas systems incapable of adaptation, e.g., non-adapting lactococcal CRISPR-Cas systems. Also provided are methods for using the compositions and products thereof to produce bacterial strains that are resistant, such as bacteriophage resistant, and/or evolved to have a desirable phenotype. Non-adapting CRISPR-Cas systems adapted according to the methods provided herein, and bacteria containing such adapted non-adapting CRISPR-Cas systems, are further provided. The compositions and methods provided herein allow for the controlled induction of natural spacer acquisition via adaptation in CRISPR-Cas systems that are fundamentally incapable of adaptation without modifying the features underlying the non-adapting behavior of the CRISPR-Cas system.

    COMPOSITION ET MÉTHODE PERMETTANT L'ÉDITION GÉNOMIQUE
    3.
    发明申请
    COMPOSITION ET MÉTHODE PERMETTANT L'ÉDITION GÉNOMIQUE 审中-公开

    公开(公告)号：WO2022269037A1

    公开(公告)日：2022-12-29

    申请号：PCT/EP2022/067351

    申请日：2022-06-24

    申请人： QUIDDITAS SA

    发明人： CHERBONNEAU, François

    IPC分类号： C12N15/10 ,  C12N15/90 ,  C12N15/102 ,  C12N15/907

    摘要： L'invention concerne un complexe moléculaire comprenant - une première molécule d'acides nucléiques simple brin comprenant au moins deux demi-sites de liaison d'une transposase, et - une deuxième molécule d'acides nucléiques simple brin au moins un demi-site de liaison d'une transposase, ledit complexe étant tel que les première et deuxième molécules d'acides nucléiques simple brin sont appariées selon la complémentarité des bases définie par Watson et Crick de sorte à définir deux sites double brin de liaison de ladite transposase. L'invention concerne par ailleurs l'utilisation de ce complexe, en particulier pour l'édition de l'ADN.