公开(公告)号：WO2021163030A2

公开(公告)日：2021-08-19

申请号：PCT/US2021/017210

申请日：2021-02-09

申请人： NOVOZYMES A/S ,  ELLIOTT, Sarah Schultheis

发明人： KRUSE, Marie Thryoese ,  LANDVIK, Sara Maria ,  HOFF, Tine ,  LI, Ming ,  SUN, Tianqi

IPC分类号： C12N9/26 ,  C12N9/2417 ,  C12N9/242 ,  C12P7/06

摘要： The present invention relates to polypeptides having alpha-amylase activity, alpha-amylase catalytic domains, and starch binding modules, and polynucleotides encoding the polypeptides, alpha-amylase catalytic domains, and starch binding modules, and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, alpha-amylase catalytic domains, and starch binding modules. The present invention relates to processes for producing fermentation products from starch-containing material. The invention also relates to an enzyme blend or composition, or a recombinant host cell or fermenting organism suitable for use in a process of the invention.

公开(公告)号：WO2022066386A2

公开(公告)日：2022-03-31

申请号：PCT/US2021/048799

申请日：2021-09-02

申请人： BASF SE ,  QURESHI, Asfia

发明人： ANAND, Priya ,  DENNIS, Jared ,  URBINA, Hugo ,  SPECIALE, Gaetano

IPC分类号： C12N9/26 ,  A21D8/04 ,  A23K10/14 ,  A23L29/30 ,  C11D3/386 ,  C12N9/28 ,  C12N9/44 ,  C12N9/96 ,  C12P19/14 ,  A21D8/042 ,  A23L29/06 ,  C11D3/38681 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/2451 ,  C12Y302/01001

摘要： The present invention relates to variants of an alpha-amylase which have an increased solubility at pH 6.0, an increased solubility at pH 10.0, a higher resistance to protein aging, and/or an increased specific activity compared to the parent alpha-amylase. The present invention also relates to methods of making the variant alpha-amylase and the use of the variant alpha-amylase in processing starch, cleaning or washing textiles, hard surfaces, or dishes, making ethanol, treating an oil well, processing pulp or paper, animal feed, syrup production, and preparing a dough or a baked product prepared from the dough.

公开(公告)号：WO2022214705A1

公开(公告)日：2022-10-13

申请号：PCT/EP2022/059587

申请日：2022-04-11

申请人： CILIAN AG

发明人： HARTMANN, Marcus Wolf William ,  ALDAG, Ingo ,  ROSSDORF, Jan ,  UTHOFF, Stefan ,  SCHUMACHER, Florian

IPC分类号： C07K14/44 ,  C12N9/20 ,  C12N9/28 ,  C12N9/50 ,  C12N15/79 ,  C12N9/2417

摘要： The present invention relates to a method for purifying proteins, which proteins are produced by homologous or heterologous expression in a ciliate host, from harvested cell culture fluid (HCCF) which was not chromatographically purified. The method comprises the steps of incubating a harvested cell culture fluid with a kosmotropic agent, efflorescing protein by formation of crystals, and, optionally, harvesting effloresced protein.

公开(公告)号：WO2021148064A1

公开(公告)日：2021-07-29

申请号：PCT/CZ2021/050007

申请日：2021-01-21

申请人： USTAV MAKROMOLEKULARNI CHEMIE AV CR, V.V.I. ,  BIOMOSAE

发明人： BENES, Hynek ,  BUJOK, Sonia ,  PARUZEL, Aleksandra ,  ZAJICOVA, Veronika ,  HNATKOVA, Tereza ,  BUSSCHER, Guuske Frederike ,  SCHMEETS, Alex Alois Joseph

IPC分类号： C08G18/16 ,  C08G18/18 ,  C08G18/24 ,  C08G18/32 ,  C08G18/40 ,  C08G18/42 ,  C08G18/64 ,  C08G18/66 ,  C08G18/73 ,  C08G18/79 ,  C08J9/08 ,  C12N1/20 ,  C12R1/00 ,  C12N9/42 ,  C12N11/093 ,  C12N9/24 ,  C08G18/165 ,  C08G18/1808 ,  C08G18/246 ,  C08G18/3218 ,  C08G18/4081 ,  C08G18/425 ,  C08G18/6484 ,  C08G18/6644 ,  C08G18/792 ,  C08G2110/0058 ,  C08G2230/00 ,  C08J2203/02 ,  C08J2300/16 ,  C08J2375/04 ,  C12N1/00 ,  C12N9/2411 ,  C12N9/2417 ,  C12N9/2442 ,  C12R2001/00

摘要： The present invention relates to a method of synthesis of a biodegradable polyurethane foam, comprising the following steps: i) mixing of at least one aliphatic isocyanate bearing at least two isocyanate groups with at least one saccharide in weight ratio of from 1:1 to 30:1 to form a saccharide-isocyanate precursor; ii) homogenization of the saccharide-isocyanate precursor from step i) with 20 to 70 % (w/w) of at least one biodegradable aliphatic polyester-ether polyol having molecular mass in the range of from 0 to 10 000 g/mol and hydroxyl number from 10 to 100 mgKOH/g, 1 to 5 % (w/w) of water, and 1 to 10 % (w/w) of at least one blowing agent other than water, related to the total weight of the resulting mixture; iii) foaming of the homogenized mixture from step ii) at the temperature of from 20 to 100 °C, resulting in the biodegradable polyurethane foam comprising incorporated saccharide. The invention further relates to a biodegradable polyurethane foam obtainable by this method, to a biodegradable polyurethane foam-based material for saccharide-cleaving enzyme production and to the use thereof.

公开(公告)号：WO2021163011A2

公开(公告)日：2021-08-19

申请号：PCT/US2021/017173

申请日：2021-02-09

申请人： NOVOZYMES A/S ,  ELLIOTT, Sarah Schultheis

发明人： ANDERSEN, Carsten ,  POULSEN, Thomas Agersten ,  NISSEN, Dorota ,  KRUSE, Marie Thryoese ,  FUKUYAMA, Shiro

IPC分类号： C12N9/18 ,  C07K2319/02 ,  C07K2319/21 ,  C12N9/2417 ,  C12Y302/01001

摘要： The present invention relates to isolated alpha-amylase variants of a parent alpha-amylase, comprising an alteration at one or more positions corresponding to positions 196, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 28, 38, 39, 43, 54, 56, 57, 64, 67, 68, 70, 71, 86, 89, 90, 94, 96, 99, 101, 103, 107, 108, 110, 113, 114, 117, 127, 134, 138, 142, 150, 151, 152, 156, 169, 171, 174, 179, 183, 193, 199, 200, 204, 205, 207, 208, 209, 212, 218, 221, 222, 224, 233, 241, 245, 259, 275, 278, 281, 282, 283, 284, 285, 308, 323, 335, 348, 359, 382, 386, 388, 392, 394, 396, 412, 414, 417, 424, 428, 457, 459, 466, 479, 489, 511, 533, 534, 542, 543, 545, 547, 549, 550, 551, 560, 566, 570, 574, 575, 576, 577, 578, 580, 581, 582, 589, 592, 599, 603, 605, 608, 614, 619, or 626 of the polypeptide of SEQ ID NO: 1, wherein the variant has alpha-amylase activity and wherein the variant has at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity, but less than 100% sequence identity, to the polypeptide of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4. The alpha-amylase variants of the invention have increased stability at pH 4.0 and 32°C compared to the parent alpha-amylase.

公开(公告)号：WO2021123184A2

公开(公告)日：2021-06-24

申请号：PCT/EP2020/087043

申请日：2020-12-18

申请人： NOVOZYMES A/S

发明人： ANDERSEN, Carsten ,  SAINATHAN, Rajendra, Kulothungan ,  MAHANKALI, Madhupriya ,  KRISHNA, Subith ,  PRASAD, Dinesh

IPC分类号： C12N9/26 ,  C12N9/28 ,  C12N9/2417 ,  C12Y302/01001

摘要： The present invention relates to alpha-amylase variants of a parent alpha-amylase having an improved wash performance when compared to the parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants, nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and method of producing the variants of the present invention.

公开(公告)号：WO2022216801A1

公开(公告)日：2022-10-13

申请号：PCT/US2022/023637

申请日：2022-04-06

申请人： DUPONT NUTRITION BIOSCIENCES APS ,  DANISCO US INC ,  DOIG, Steven ,  FRISCH, Ryan ,  HAANING, Svend ,  WOUTER KOOPMAN, Frank ,  MATTHIAS KRAGH, Karsten ,  KRAGH, Lene ,  LEEFLANG, Chris ,  PRICELIUS, Sina

发明人： DOIG, Steven ,  FRISCH, Ryan ,  HAANING, Svend ,  WOUTER KOOPMAN, Frank ,  MATTHIAS KRAGH, Karsten ,  KRAGH, Lene ,  LEEFLANG, Chris ,  PRICELIUS, Sina

IPC分类号： C12N9/26 ,  C12P19/14 ,  A21D8/04 ,  A23L33/18 ,  A23L29/30 ,  C12N9/28 ,  A21D8/042 ,  A23L29/06 ,  C12N9/2411 ,  C12N9/2414 ,  C12N9/2417 ,  C12P19/00 ,  C12Y302/0106

摘要： This invention relates to polypeptides, more specifically amylase polypeptides and nucleic acids encoding these, and their uses e.g. as non-maltogenic exoamylases in producing food or feed products.

公开(公告)号：WO2022171780A2

公开(公告)日：2022-08-18

申请号：PCT/EP2022/053334

申请日：2022-02-11

申请人： NOVOZYMES A/S

发明人： ANDERSEN, Carsten

IPC分类号： C12N9/28 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C12N9/2417

摘要： The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：WO2022162559A2

公开(公告)日：2022-08-04

申请号：PCT/IB2022/050686

申请日：2022-01-26

申请人： DANSTAR FERMENT AG

发明人： WANG, Zhiqing ,  RANADE, Aditi ,  ARGYROS, Aaron ,  FROEHLICH, Allan ,  RICE, Charles F.

IPC分类号： C07K14/395 ,  C12N9/18 ,  C12N9/28 ,  C12N9/60 ,  C12N9/90 ,  C12P21/02 ,  C12R1/865 ,  C12N9/2417 ,  C12R2001/865

摘要： The present disclosure concerns recombinant yeast host cells having a first genetic modification to express a heterologous polypeptide or to over-express a native polypeptide. The recombinant yeast host cells also have a second genetic modification to at least partially mitigate the reduction in growth rate resulting from the expression of the heterologous polypeptide or the over-expression of the native polypeptide. The second genetic modification can be, for example, to favor the secretion of the heterologous or native polypeptide.

公开(公告)号：WO2021248045A2

公开(公告)日：2021-12-09

申请号：PCT/US2021/035968

申请日：2021-06-04

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  BASF SE

发明人： LISZKA, Michael ,  BATZILLA, Alina ,  WU, Zachary ,  ARNOLD, Frances

IPC分类号： C12N9/42 ,  C12N9/24 ,  C12N9/26 ,  C12P19/14 ,  C12P7/56 ,  C07K7/08 ,  A61P43/00 ,  C07K2319/02 ,  C12N9/14 ,  C12N9/2411 ,  C12N9/2417 ,  C12N9/2482 ,  C12N9/52 ,  C12N9/54 ,  C12Y302/01001 ,  C12Y302/01008 ,  C12Y304/21062 ,  C12Y308/01005

摘要： The disclosure provides for artificial signal peptides generated by systems and methods utilizing deep learning.