利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

16 条记录 (耗时 0.016 秒)

    TETRAHYMENA HEAT INDUCIBLE PROMOTERS AND THEIR USE
    1.
    发明申请
    TETRAHYMENA HEAT INDUCIBLE PROMOTERS AND THEIR USE 审中-公开
    四氯乙烯热诱导促进剂及其使用

    公开(公告)号：WO2007006812A1

    公开(公告)日：2007-01-18

    申请号：PCT/EP2006/064244

    申请日：2006-07-13

    申请人： CILIAN AG ,  HARTMANN, Marcus ,  WEIDE, Thomas ,  HERRMANN, Lutz ,  WOLF, Nadine

    发明人： HARTMANN, Marcus ,  WEIDE, Thomas ,  HERRMANN, Lutz ,  WOLF, Nadine

    IPC分类号： C12N15/85

    CPC分类号： C07K14/44 ,  C12N2830/002

    摘要： The present invention relates to heat-inducible promoters of the heat shock protein family of the ciliate Tetrahymena thermophila, especially a promoter having the nucleotide sequence of Seq. ID No. 1 or promoter-effective fragments thereof. Furthermore the use of heat-inducible promoters of the present invention for the expression of homologous and/or heterologous proteins in the ciliate Tetrahymena thermophila is claimed.

    摘要翻译： 本发明涉及嗜热四膜虫嗜热细菌的热休克蛋白家族的热诱导型启动子，特别是具有Seq的核苷酸序列的启动子。 1号或其启动子有效片段。 此外，要求使用本发明的热诱导型启动子在嗜中性四膜虫嗜热细菌中表达同源和/或异源蛋白质。

    SCREENINGVERFAHREN ZUR IDENTIFIZIERUNG PROTEASE-SEKRETIONSDEFIZIENTER MUTANTEN VON MIKROORGANISMEN
    2.
    发明申请
    SCREENINGVERFAHREN ZUR IDENTIFIZIERUNG PROTEASE-SEKRETIONSDEFIZIENTER MUTANTEN VON MIKROORGANISMEN 审中-公开
    筛选方法，微生物中识别蛋白酶突变体SEKRETIONSDEFIZIENTER

    公开(公告)号：WO2006087366A1

    公开(公告)日：2006-08-24

    申请号：PCT/EP2006/060034

    申请日：2006-02-16

    申请人： CILIAN AG ,  HARTMANN, Marcus ,  BROERMANN, André

    发明人： HARTMANN, Marcus ,  BROERMANN, André

    IPC分类号： C12Q1/37

    CPC分类号： C12Q1/37 ,  C12Q1/04

    摘要： Gegenstand der Erfindung ist ein Verfahren zur Identifizierung eines Protease- sekretionsdefizienten Stammes eines Mikroorganismus, wobei eine Mutante des Ciliaten zu einem Gel gegeben wird, inkubiert wird unter Bedingungen, unter denen die Mutante des Ciliaten Proteine sekretiert, die Mutante des Ciliaten vom Gel abgetrennt wird, wobei in dem Gel mindestens ein Substrat für mindestens eine Sekretionsprotease des Ciliaten enthalten ist und/oder das Gel selbst das Substrat ist und/oder das Substrat später zugesetzt wird und mindestens teilweise in das Gel hineindiffundiert, und die Proteaseaktivität auf das Substrat gemessen wird.

    摘要翻译： 本发明是鉴定微生物的蛋白酶sekretionsdefizienten株，纤毛虫的突变体被加入到凝胶在其下分泌的蛋白质的纤毛虫的突变体，纤毛虫的突变体从凝胶中分离的条件下温育的方法， 其中对于至少一个纤毛虫Sekretionsprotease在凝胶至少一个基片载和/或凝胶本身和基片/或衬底以后添加并至少部分地扩散到凝胶和蛋白酶活性在衬底上测定。

    USE OF ENZYMES WITH A WIDE PH ACTIVITY RANGE AS MEDICAMENTS FOR PROMOTING DIGESTION
    3.
    发明申请
    USE OF ENZYMES WITH A WIDE PH ACTIVITY RANGE AS MEDICAMENTS FOR PROMOTING DIGESTION 审中-公开
    以广泛的PH活动范围作为促进消费的药物使用酶

    公开(公告)号：WO2016116600A1

    公开(公告)日：2016-07-28

    申请号：PCT/EP2016/051341

    申请日：2016-01-22

    申请人： CILIAN AG

    发明人： HARTMANN, Marcus Wolf William ,  ALDAG, Ingo

    IPC分类号： A61K38/46 ,  C12N9/20 ,  C11D3/386

    CPC分类号： C12N9/20 ,  A61K38/465 ,  C12Y301/00

    摘要： Disclosed is a combination of two or more lipase enzymes, and its use for treating a lipid digestion deficiency and /or a digestive disorder. At least one lipase enzyme has a pH optimum at an acidic pH value, while at least one other lipase enzyme has a pH optimum at an alkalic pH value.

    摘要翻译： 公开了两种或更多种脂肪酶的组合及其用于治疗脂肪消化缺陷和/或消化障碍的用途。 至少一种脂肪酶在酸性pH值下具有最佳pH，而至少一种其它脂肪酶在碱性pH值下具有最佳pH。

    DNA SEQUENCES OF MAJOR SECRETED PROTEINS FROM THE CILIATE TETRAHYMENA AND USE THEREOF
    5.
    发明申请
    DNA SEQUENCES OF MAJOR SECRETED PROTEINS FROM THE CILIATE TETRAHYMENA AND USE THEREOF 审中-公开

    公开(公告)号：WO2003078566A3

    公开(公告)日：2003-09-25

    申请号：PCT/EP2003/002856

    申请日：2003-03-19

    申请人： CILIAN AG ,  HARTMANN, Marcus ,  WOLF, Nadine

    发明人： HARTMANN, Marcus ,  WOLF, Nadine

    IPC分类号： C12N15/79

    摘要： A regulatory element of a DNA for an efficient heterologous expression of proteins in Tetrahymena ssp which efficient heterologous expression is performed under control of promotors and/or terminators which are derived from in Tetrahymena ssp naturally occurring DNA comprising promotors and/or terminators and a coding region for proteins secreted ion a high level and the expression of proteins secreted on a high level is independent of the cell-cycle of Tetrahymena ssp.Furthermore a method is disclosed for the heterologous expression of proteins from Tetrahymena using gene constructs made from regulatory elements selected from the group consisting of promoters or terminators from Tetrahymena and coding nucleic acid sequences of a protein to be expressed heterologously, said regulatory elements from Tetrahymena being obtainable by: two-dimensional gel electrophoretical separation and isolation of the proteins (CMSP) selected from the group according to table 1; determination of at least one partial amino acid sequence of the proteins; establishing the nucleic acid sequence of the proteins and therefrom establishing the gene which codes for these proteins; establishing the regulatory elements of the coding region of said proteins.

    NEW LIPASE ENZYME
    8.
    发明申请
    NEW LIPASE ENZYME 审中-公开

    公开(公告)号：WO2021260148A1

    公开(公告)日：2021-12-30

    申请号：PCT/EP2021/067419

    申请日：2021-06-24

    申请人： CILIAN AG

    发明人： MOERS, Christopher ,  ALDAG, Ingo

    IPC分类号： C12N9/20 ,  A61P1/18

    摘要： The present invention relates to a Lipase enzyme comprising an amino acid sequence that has at least 90 % sequence identity with SEQ ID NO 1, or comprising a functional fragment thereof encompassing position Q55, with the proviso that the lipase enzyme has a substitution of an amino acid with a basic side chain at neutral pH in position Q55 (Fig. 1).