公开(公告)号：WO2016116600A1

公开(公告)日：2016-07-28

申请号：PCT/EP2016/051341

申请日：2016-01-22

申请人： CILIAN AG

发明人： HARTMANN, Marcus Wolf William ,  ALDAG, Ingo

IPC分类号： A61K38/46 ,  C12N9/20 ,  C11D3/386

CPC分类号： C12N9/20 ,  A61K38/465 ,  C12Y301/00

摘要： Disclosed is a combination of two or more lipase enzymes, and its use for treating a lipid digestion deficiency and /or a digestive disorder. At least one lipase enzyme has a pH optimum at an acidic pH value, while at least one other lipase enzyme has a pH optimum at an alkalic pH value.

摘要翻译： 公开了两种或更多种脂肪酶的组合及其用于治疗脂肪消化缺陷和/或消化障碍的用途。 至少一种脂肪酶在酸性pH值下具有最佳pH，而至少一种其它脂肪酶在碱性pH值下具有最佳pH。

公开(公告)号：WO2022214705A1

公开(公告)日：2022-10-13

申请号：PCT/EP2022/059587

申请日：2022-04-11

申请人： CILIAN AG

发明人： HARTMANN, Marcus Wolf William ,  ALDAG, Ingo ,  ROSSDORF, Jan ,  UTHOFF, Stefan ,  SCHUMACHER, Florian

IPC分类号： C07K14/44 ,  C12N9/20 ,  C12N9/28 ,  C12N9/50 ,  C12N15/79 ,  C12N9/2417

摘要： The present invention relates to a method for purifying proteins, which proteins are produced by homologous or heterologous expression in a ciliate host, from harvested cell culture fluid (HCCF) which was not chromatographically purified. The method comprises the steps of incubating a harvested cell culture fluid with a kosmotropic agent, efflorescing protein by formation of crystals, and, optionally, harvesting effloresced protein.

公开(公告)号：WO2021260148A1

公开(公告)日：2021-12-30

申请号：PCT/EP2021/067419

申请日：2021-06-24

申请人： CILIAN AG

发明人： MOERS, Christopher ,  ALDAG, Ingo

IPC分类号： C12N9/20 ,  A61P1/18

摘要： The present invention relates to a Lipase enzyme comprising an amino acid sequence that has at least 90 % sequence identity with SEQ ID NO 1, or comprising a functional fragment thereof encompassing position Q55, with the proviso that the lipase enzyme has a substitution of an amino acid with a basic side chain at neutral pH in position Q55 (Fig. 1).