公开(公告)号：WO1997028275A1

公开(公告)日：1997-08-07

申请号：PCT/US1997001916

申请日：1997-02-05

Applicant: IGEN INTERNATIONAL, INC. ,  MARTIN, Mark, T. ,  DONG, Liwen

Inventor： IGEN INTERNATIONAL, INC.

IPC: C12Q01/34

CPC classification number: C12Q1/34 ,  C12Q2334/00 ,  Y10S435/817 ,  Y10S435/968 ,  Y10S435/975

Abstract: A rapid single step assay suitable for the detection or quantification of enzymes, in particular, hydrolases, such as aminopeptidases and esterases is described. The enzymatic reaction causes the cleavage of a metal ligand labeled hydrolase substrate. The cleavec ligand alters the electrochemiluminescence of bidentate aromatic heterocyclic nitrogen-containing ligand reagent. The change in electrochemiluminescence correlates to the presence of hydrolase activity present in the sample. The assay can be performed on an IGEN Origen Analyzer. The figure illustrates ECL detection of mixtures of ruthenium (II) tris(bipyridyl) and various concentrations of either picolinic acid (1) (black bars) or 2-pyridinecarboxylic acid ethyl ester (4) (white bars).

Abstract translation: 描述了适用于检测或定量酶，特别是水解酶如氨基肽酶和酯酶的快速单步测定法。 酶反应导致金属配体标记的水解酶底物的裂解。 裂解配体改变二齿芳族杂环含氮配体试剂的电化学发光。 电化学发光的变化与样品中存在的水解酶活性的存在相关。 该测定可以在IGEN Origen分析仪上进行。 该图示出了钌（II）三（联吡啶）和各种浓度的吡啶甲酸（1）（黑条）或2-吡啶羧酸乙酯（4）（白色条）的混合物的ECL检测。

公开(公告)号：WO1995005479A1

公开(公告)日：1995-02-23

申请号：PCT/US1994009278

申请日：1994-08-17

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  DENNIS, Edward, A. ,  REYNOLDS, Laure, J. ,  YU, Lin

IPC: C12Q01/34

CPC classification number: C07F9/10 ,  C12Q1/44 ,  G01N2333/918

Abstract: A non-radioactive, spectrophotometric, microtiter plate assay for human cystolic phospholipase A (cPLA) is disclosed. The assay utilizes a novel synthetic thiolphospholipid analog as a substrate. In one embodiment, the substrate is a phosphatidylcholine derivative with an arachidonoylthioester in the sn-2 position and an alkyl-ether or alkenyl-ether in the sn-1 position. The alkyl-ether or alkenyl-ether in the sn-1 position of the substrate ensures that the assay will only measure PLA activity and will not be complicated by metabolism of the lysophospholipid product by the enzyme's lysophospholipase activity. As a model of the invention, the figure shows the 8 steps used to synthesize the compound 1-Hexadecyl-2-arachidonylthio-2-deoxy-sn-glycero-3-phosphorylcholine from (R)-glycidol.

Abstract translation: 公开了用于人类收缩磷脂酶A（cPLA）的非放射性，分光光度法，微量滴定板测定法。 该测定法利用新的合成硫代磷脂类似物作为底物。 在一个实施方案中，底物是具有sn-2位置的花生四烯酰硫代酯和sn-1位置的烷基醚或链烯基醚的磷脂酰胆碱衍生物。 底物sn-1位置上的烷基醚或链烯基醚确保了该测定仅测量PLA活性，并且不会因酶的溶血磷脂酶活性而导致溶血磷脂产物的代谢变得复杂。 作为本发明的模型，该图显示了从（R） - 缩水甘油合成化合物1-十六烷基-2-花生四烯基硫代-2-脱氧-sn-甘油基-3-磷酸胆碱的8个步骤。

公开(公告)号：WO1994012663A1

公开(公告)日：1994-06-09

申请号：PCT/GB1993002465

申请日：1993-11-30

Applicant: MEDICAL RESEARCH COUNCIL ,  JOHNSON, Martin, Keith

Inventor： MEDICAL RESEARCH COUNCIL

IPC: C12Q01/34

CPC classification number: C07K5/06191 ,  C07F9/6571 ,  C07F9/657127 ,  C07F9/657154 ,  C07F9/65744 ,  C07J51/00 ,  C07K1/1077 ,  C12Q1/37 ,  C12Q1/44

Abstract: A reagent for the identification or purification of a serine esterase enzyme comprising an organophosphorus serine esterase inhibitor to which is bonded a reporter group. Particularly preferred is a compound of formula (II), where R is hydrogen, or an alkyl, aryl, alkoxy, acyl, halogen or nitro group or a fused aromatic ring; B-R is C = O or CHR where R is hydrogen or an alkyl, aryl or cyano group; X is O, S, NH or NR; A is a reporter group.

Abstract translation: 用于鉴定或纯化丝氨酸酯酶的试剂，其包含结合了报告基团的有机磷丝氨酸酯酶抑制剂。 特别优选式（II）的化合物，其中R 1是氢或烷基，芳基，烷氧基，酰基，卤素或硝基或稠合芳环; B-R 2是C = O或CHR 2，其中R 2是氢或烷基，芳基或氰基; X是O，S，NH或NR; A是记者组。

公开(公告)号：WO1994005805A1

公开(公告)日：1994-03-17

申请号：PCT/FI1993000355

申请日：1993-09-07

Applicant: MONONEN, Ilkka ,  KAARTINEN, Vesa

IPC: C12Q01/34

CPC classification number: C12Q1/34 ,  G01N2333/978

Abstract: The present invention concerns a method of assaying the activity of asparagine- beta -amido hydrolase enzymes in biological samples. In the method, the sample contacted with a compound which contains an easily detectable moiety attached to the beta -amino group of L-asparagine or beta -carboxyl group of L-aspartic acid, the easily detectable moiety being released by the enzyme present in the biological sample, and the released compound is determined. The invention further concerns an assay kit for determination of the activity of asparagine- beta -amidohydrolase enzymes in biological samples.

Abstract translation: 本发明涉及一种测定生物样品中天冬酰胺-β-酰氨基水解酶的活性的方法。 在该方法中，样品与含有易于检测的部分接触，该化合物含有L-天冬酰胺或L-天冬氨酸的β-羧基的β-氨基，易于检测的部分被存在于 生物样品和释放的化合物。 本发明还涉及用于测定生物样品中天冬酰胺-β-酰氨基水解酶的活性的测定试剂盒。

公开(公告)号：WO1993010260A1

公开(公告)日：1993-05-27

申请号：PCT/US1992010028

申请日：1992-11-18

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD ...

Inventor： THE BOARD OF TRUSTEES OF THE LELAND STANFORD ... ,  GOOCHEE, Charles, F. ,  GRAMER, Michael, J.

IPC: C12Q01/34

CPC classification number: C07K14/46 ,  C12N9/2402 ,  C12P19/14 ,  C12Y302/01018

Abstract: A neuraminidase enzyme with unusually high activity under extracellular cell-culture conditions has been discovered in Chinese hamster ovary (CHO) cells and other types of cells. Knowledge of the existence of this enzyme allows greater control over post-expression, extracellular oligosaccharide degradation than was previously possible in the absence of this discovery by using standard processing steps selected specifically to control the newly discovered activity and/or by using neuraminidase inhibitors.

Abstract translation: 已经在中国仓鼠卵巢（CHO）细胞和其他类型的细胞中发现了细胞外细胞培养条件下异常高活性的神经氨酸酶。 通过使用特别选择的标准处理步骤和/或使用神经氨酸酶抑制剂，知道这种酶的存在允许更好地控制后表达，细胞外寡糖降解，比先前在不存在该发现的情况下可能的那样。

公开(公告)号：WO1992021777A1

公开(公告)日：1992-12-10

申请号：PCT/US1992004596

申请日：1992-05-22

Applicant: CHEN, Kirk, C., S.

IPC: C12Q01/34

CPC classification number: G01N31/22 ,  Y10S435/871 ,  Y10S435/933 ,  Y10S435/973 ,  Y10S436/904

Abstract: A novel colorimetric method for the determination of penicilloic acids is disclosed. The novel method is based on the discovery described in this invention. The chromophore formed by oxidation of either the N-alkyl derivative of p-phenylenediamine or the 3,3',5,5'-tetraalkyl derivative of benzidine can be decolorized by the penicilloic acids in the presence of a mercury-containing compound. None of the intact penicillin antibiotics can decolorize the chromophore in the presence of the mercury-containing compound. The final concentration of the mercury-containing compound in the decolorization mixture ranges from approximately 0.005 mM to approximately 3 mM, and the pH of the decolorization mixture ranges form 3.5 to 8.0.

公开(公告)号：WO1997027320A1

公开(公告)日：1997-07-31

申请号：PCT/US1997000883

申请日：1997-01-23

Applicant: BOEHRINGER MANNHEIM CORPORATION ,  POWELL, Michael, J. ,  KHANNA, Pyare ,  EISENBEIS, Scott, J. ,  LINGENFELTER, David

Inventor： BOEHRINGER MANNHEIM CORPORATION

IPC: C12Q01/34

CPC classification number: C12Q1/37

Abstract: Formation of an intramolecular cross-link in enzyme donor polypeptide fragments of beta -galactosidase, thereby forming a cyclic enzyme donor which is hindered from complementation with an enzyme acceptor fragment to form active of beta -galactosidase. The cyclic enzyme donor can be linearized by cleaving to restore complementation ability. Assays in which such cyclic enzyme donors are linearized by specific analytes are disclosed, as well as novel homobifunctional bis-maleimido cross-linking agents of formula (I) wherein R is hydroxy or acetate.

Abstract translation: 在β-半乳糖苷酶的酶供体多肽片段中形成分子内交联，从而形成环状酶供体，其受到与受体片段互补以形成β-半乳糖苷酶的活性。 循环酶供体可以通过切割线性化以恢复互补能力。 公开了这样的环状酶供体通过特定分析物线性化的测定，以及式（I）的新型双官能双马来酰亚胺交联剂，其中R是羟基或乙酸酯。

公开(公告)号：WO1995004158A1

公开(公告)日：1995-02-09

申请号：PCT/US1994008207

申请日：1994-07-26

Applicant: THE UPJOHN COMPANY ,  HOOGWERF, Arlene, J. ,  LEDBETTER, Steven, R.

Inventor： THE UPJOHN COMPANY

IPC: C12Q01/34

CPC classification number: C12Y302/01166 ,  C07K14/47 ,  C12N9/2402 ,  C12Q1/34 ,  G01N2333/924

Abstract: Purified heparanase having activity of greater than 20 units/ mu g protein, preferably greater than 50 units heparanase activity per mu g protein, is described. The use of heparanase for screening for anti-heparanase compounds is also described. In addition, the use of the high potency heparanase to accelerate wound healing or its use as an immobilized heparanase filter connected to extracorporeal devices to degrade heparin and neutralize its anticoagulant properties during surgery is disclosed.

Abstract translation: 描述了具有大于20单位/μg蛋白质的活性的纯化的乙酰肝素酶，优选大于50单位每μg蛋白质的乙酰肝素酶活性。 还描述了使用乙酰肝素酶筛选抗肝素酶化合物。 此外，公开了使用高效乙酰肝素酶加速伤口愈合或其作为固定化的肝素酶过滤器连接到体外装置以降解肝素并中和其抗凝血性质在手术中。

公开(公告)号：WO1994023061A1

公开(公告)日：1994-10-13

申请号：PCT/JP1994000572

申请日：1994-04-06

Applicant: KYOWA MEDEX CO., LTD. ,  TADANO, Toshio ,  MIIKE, Akira ,  UMEMOTO, Jun

Inventor： KYOWA MEDEX CO., LTD.

IPC: C12Q01/34

CPC classification number: C12Q1/54 ,  C12Q1/34 ,  G01N33/84 ,  G01N2333/924

Abstract: A method of determining sodium ions present in a specimen by using a beta -galactosidase in an aqueous medium, characterized by conducting the enzymatic reaction in the presence of a cation that competes with the sodium ion. This method facilitates rapid determination of sodium ions with good quantitativeness and reproducibility.

Abstract translation: 通过在水性介质中使用β-半乳糖苷酶测定样品中存在的钠离子的方法，其特征在于在与钠离子竞争的阳离子存在下进行酶反应。 该方法有利于快速测定钠离子，具有良好的定量和重现性。

公开(公告)号：WO1992011386A1

公开(公告)日：1992-07-09

申请号：PCT/JP1991001732

申请日：1991-12-18

Applicant: SHOWA YAKUHIN KAKO CO., LTD. ,  INOUE, Matsuhisa ,  SAKAI, Akira

Inventor： SHOWA YAKUHIN KAKO CO., LTD.

IPC: C12Q01/34

CPC classification number: C12Q1/34 ,  G01N2333/986 ,  G01N2415/00 ,  Y10S435/81 ,  Y10S435/975

Abstract: A cephalosporinase testing agent comprising a cephalosporin antibiotic such as cephaloridin, a penicillinase inhibitor in an amount of 20 wt% or less based on the antibiotic, and a pH indicator such as bromocresol purple. A cephalosporinase producing bacterium can be judged by smearing a sample containing a test bacterium on the testing agent and observing the color tone change. According to this agent, a cephalosporinase-active penicillinase producing bacterium is judged cephalosporinase-negative by the action of a penicillinase inhibitor such as clavulanic acid. Therefore only a cephalosporinase producing bacterium is judged cephalosporinase-positive. Thus this agent is useful since it is free from misjudgment.