公开(公告)号：WO0153819A9

公开(公告)日：2002-10-17

申请号：PCT/US0101785

申请日：2001-01-18

申请人： ADVION BIOSCIENCES INC

发明人： CORSO THOMAS N ,  SCHULTZ GARY A ,  PROSSER SIMON J ,  HUANG XIAN

IPC分类号： G01N27/62 ,  B01D15/08 ,  B01D57/02 ,  B01D59/44 ,  B01J20/281 ,  B01J20/283 ,  B01J20/285 ,  B03C5/00 ,  B05B5/043 ,  G01N1/10 ,  G01N21/33 ,  G01N21/53 ,  G01N21/64 ,  G01N27/447 ,  G01N30/28 ,  G01N30/46 ,  G01N30/52 ,  G01N30/60 ,  G01N30/72 ,  G01N30/74 ,  G01N30/88 ,  G01N37/00 ,  H01J49/00 ,  H01J49/04 ,  H01J49/10 ,  G01N27/26 ,  B32B31/00 ,  H01J49/14 ,  G01N27/477

CPC分类号： G01N30/466 ,  B01J20/261 ,  B01J20/267 ,  B01J20/281 ,  B01J20/285 ,  B01J2220/54 ,  B01J2220/82 ,  G01N27/44717 ,  G01N30/463 ,  G01N30/467 ,  G01N30/48 ,  G01N30/6095 ,  G01N30/7266 ,  G01N2030/285 ,  G01N2030/528 ,  H01J49/0018 ,  H01J49/167

摘要： A microfabricated silicon chip with a separation material, such as in situ prepared porous polymer monoliths (254) in its microchannels is disclosed. The polymer monoliths are liquid-permeable and serve as microcolumns for liquid chromatography, which are prepared by in situ radical polymerization of a mixture containing vinyl monomers and solvents (porogen) in the microchannels. A method and system are disclosed to generate one or more electrospray plumes from one or more nozzles that provide an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analyses contained within the fluid. A plurality of electrospray devices (250) can be used in the form of an array of miniaturized separate electrospray devices for the purpose of generating multiple electrospray plumes from multiple nozzles (242) for the same fluid for analysis.

摘要翻译： 公开了一种具有分离材料的微制造硅芯片，例如其微通道中原位制备的多孔聚合物整料（254）。 聚合物整料是液体可渗透的，用作液相色谱的微柱，其通过在微通道中含有乙烯基单体和溶剂（致孔剂）的混合物的原位自由基聚合制备。 公开了一种方法和系统，以从一个或多个喷嘴产生一个或多个电喷雾羽流，其提供与质量分析仪测量的离子强度，其大小与为流体中包含的分析形成的电喷雾羽流的数量成比例。 多个电喷射装置（250）可以以小型化的独立电喷雾装置阵列的形式使用，用于从用于分析的相同流体的多个喷嘴（242）产生多个电喷雾羽流。

公开(公告)号：WO01053819A1

公开(公告)日：2001-07-26

申请号：PCT/US2001/001785

申请日：2001-01-18

IPC分类号： G01N27/62 ,  B01D15/08 ,  B01D57/02 ,  B01D59/44 ,  B01J20/281 ,  B01J20/283 ,  B01J20/285 ,  B03C5/00 ,  B05B5/043 ,  G01N1/10 ,  G01N21/33 ,  G01N21/53 ,  G01N21/64 ,  G01N27/447 ,  G01N30/28 ,  G01N30/46 ,  G01N30/52 ,  G01N30/60 ,  G01N30/72 ,  G01N30/74 ,  G01N30/88 ,  G01N37/00 ,  H01J49/00 ,  H01J49/04 ,  H01J49/10 ,  G01N27/26 ,  B32B31/00 ,  H01J49/14 ,  G01N27/477

CPC分类号： G01N30/466 ,  B01J20/261 ,  B01J20/267 ,  B01J20/281 ,  B01J20/285 ,  B01J2220/54 ,  B01J2220/82 ,  G01N27/44717 ,  G01N30/463 ,  G01N30/467 ,  G01N30/48 ,  G01N30/6095 ,  G01N30/7266 ,  G01N2030/285 ,  G01N2030/528 ,  H01J49/0018 ,  H01J49/167

摘要： A microfabricated silicon chip with a separation material, such as in situ prepared porous polymer monoliths (254) in its microchannels is disclosed. The polymer monoliths are liquid-permeable and serve as microcolumns for liquid chromatography, which are prepared by in situ radical polymerization of a mixture containing vinyl monomers and solvents (porogen) in the microchannels. A method and system are disclosed to generate one or more electrospray plumes from one or more nozzles that provide an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analyses contained within the fluid. A plurality of electrospray devices (250) can be used in the form of an array of miniaturized separate electrospray devices for the purpose of generating multiple electrospray plumes from multiple nozzles (242) for the same fluid for analysis.

摘要翻译： 公开了一种具有分离材料的微制造硅芯片，例如其微通道中原位制备的多孔聚合物整料（254）。 聚合物整料是液体可渗透的，用作液相色谱的微柱，其通过在微通道中含有乙烯基单体和溶剂（致孔剂）的混合物的原位自由基聚合制备。 公开了一种方法和系统，以从一个或多个喷嘴产生一个或多个电喷雾羽流，其提供与质量分析仪测量的离子强度，其大小与为流体中包含的分析形成的电喷雾羽流的数量成比例。 多个电喷射装置（250）可以以小型化的独立电喷雾装置阵列的形式使用，用于从用于分析的相同流体的多个喷嘴（242）产生多个电喷雾羽流。

公开(公告)号：WO1996023221A1

公开(公告)日：1996-08-01

申请号：PCT/US1996001347

申请日：1996-01-23

申请人： BECKMAN INSTRUMENTS, INC. ,  NORTHEASTERN UNIVERSITY ,  NORTHEASTERN UNIVERSITY ,  KARGER, Barry, L. ,  GOETZINGER, Wolfgang ,  MUTH, Jochen ,  SHIEH, Chia-Hui

发明人： BECKMAN INSTRUMENTS, INC. ,  NORTHEASTERN UNIVERSITY ,  NORTHEASTERN UNIVERSITY

IPC分类号： G01N27/477

CPC分类号： G01N27/44704 ,  G01N27/44752

摘要： Coated capillary electrophoresis columns and methods for their use in electrophoretic separations are disclosed. The coated capillary columns include a length of tubing having an interior surface having an interconnected polymeric coating. The interconnected polymeric coating includes a hydrophobic hydrocarbon polymeric functionality covalently bound to the interior surface and polyvinylalcohol interconnected with the hydrophobic polymeric functionality. Exemplary columns are prepared by causing a Si-OH reactive compound, having a hydrophobic polymeric functionality, to react with Si-OH functionalities on the interior surface of capillary columns. Then causing vinyl acetate to polymerize in contact with the hydrophobic polymeric functionality and hydrolyzing the resulting polyvinylacetate forms a coating of interconnecting hydrophilic and hydrophobic polymers. Alternative exemplary coatings include a modified polyvinylalcohol such as a polyvinylether.

摘要翻译： 公开了涂布毛细管电泳柱及其用于电泳分离的方法。 涂覆的毛细管柱包括具有内表面的一段长度的管，其具有互连的聚合物涂层。 相互连接的聚合物涂层包括与内表面共价结合的疏水性烃聚合物官能团和与疏水性聚合物官能团互连的聚乙烯醇。 通过使具有疏水聚合官能度的Si-OH反应性化合物与毛细管柱内表面上的Si-OH官能团反应来制备示例性柱。 然后使乙酸乙烯酯与疏水聚合官能团接触聚合并水解所得聚乙酸乙烯酯形成互连的亲水和疏水聚合物的涂层。 替代的示例性涂层包括改性的聚乙烯醇，例如聚乙烯醚。