公开(公告)号：WO2018183592A1

公开(公告)日：2018-10-04

申请号：PCT/US2018/024997

申请日：2018-03-29

Applicant: PRINCETON BIOCHEMICALS, INC. ,  GUZMAN, Norberto ,  GUZMAN, Daniel, E.

Inventor： GUZMAN, Norberto ,  GUZMAN, Daniel, E.

IPC: B01L3/00 ,  G01N1/40 ,  G01N33/537

CPC classification number: B01L3/502707 ,  B01L2200/026 ,  B01L2200/028 ,  B01L2300/0838 ,  B01L2300/0861 ,  G01N1/40 ,  G01N1/405 ,  G01N27/44704 ,  G01N27/44743 ,  G01N33/54326 ,  G01N2001/4038

Abstract: The present invention relates to an immunoaffinity device for capturing, isolating and purifying one or more analytes of interest present at high or low concentration in simple or complex matrices. The device is designed as an integrated modular unit that includes one or more analyte concentrator-microreactor devices anchored into a T-shaped support box, which is built-in or connected to a interchangeable cartridge-cassette of a capillary electrophoresis or liquid chromatography apparatus for the isolation, enrichment, derivatization, separation and characterization of small molecules and polymeric macromolecules, primarily protein and peptide biomarkers. The integrated modular unit is also designed to perform metabolic or bioactivity studies.

公开(公告)号：WO2017132630A1

公开(公告)日：2017-08-03

申请号：PCT/US2017/015519

申请日：2017-01-28

Applicant: PURIGEN BIOSYSTEMS, INC.

Inventor： MARSHALL, Lewis, A. ,  HIDDESSEN, Amy, L. ,  HOVERTER, Nathan, P. ,  ROSE, Klint, A. ,  SANTIAGO, Juan, G.

IPC: C12N15/10 ,  G01N27/447 ,  B81B1/00

CPC classification number: B81B1/00 ,  C12N15/10 ,  C12N15/101 ,  G01N27/44704 ,  G01N27/44791 ,  C12Q2565/125

Abstract: The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.

Abstract translation: 本公开涉及用于处理，提取或纯化一种或多种分析物的流体系统和装置。 这些系统和装置可用于处理样品和提取核酸，例如通过等速电泳。 具体而言，所述系统和相关方法可以允许从样品如组织或细胞中提取核酸，包括非交联的核酸。 系统和设备也可用于多路并行采样处理。

公开(公告)号：WO2017002239A1

公开(公告)日：2017-01-05

申请号：PCT/JP2015/069040

申请日：2015-07-01

Applicant: 株式会社日立ハイテクノロジーズ

Inventor： 奥野　恵佳 ,  山崎　基博 ,  中澤　太朗 ,  宮崎　充弘 ,  木村　隆介

IPC: G01N27/447

CPC classification number: G01N27/44708 ,  G01N27/44704

Abstract: キャピラリカートリッジにおいて、取り付け性の改善と短時間分析を実現するための放熱性能向上の両立を達成する。その一部に検出部０６が設けられたキャピラリ０２と、キャピラリ０２を支持する板状の支持体０３との間に放熱体０４を設け、放熱体０４によりキャピラリ０２内部の温度上昇を抑えることで、発熱量が大きくなる高電圧印加条件下での電気泳動を可能にし、分析時間を短縮する。また、キャピラリ０２と支持体０３と放熱体０４を一体化させた構造にして取り付けの際の固定箇所を検出部０６と電極ホルダ０５だけに減らすことで操作の煩雑さを改善することができる。

Abstract translation: 在本发明中，毛细管盒实现了增强的附着性和增强的散热以实现快速分析。 散热体04设置在具有设置在其一部分上的检测单元06的毛细管02和用于支撑毛细管02的板状支撑体03之间。使用散热体04抑制毛细管02内的温度升高，进行电泳 在高电压应用条件下，可以增加发热量并缩短分析时间。 此外，采用毛细管02，支撑体03和散热体04一体化的构造减少了在仅附着到检测单元06和电极夹持器05的固定位置，从而可以减轻操作复杂性。

公开(公告)号：WO2016065073A1

公开(公告)日：2016-04-28

申请号：PCT/US2015/056764

申请日：2015-10-21

Applicant: INTEGENX INC.

Inventor： KINDWALL, Alexander ,  PARK, Chungsoo, Charles ,  ILKOV, Fedor ,  JOVANOVICH, Stevan ,  KING, David ,  LITTLEHALE, Tristan, Robert ,  NILSON, David, Grant ,  PITTARO, Rick ,  SCHUEREN, Robert, A. ,  VANGBO, Mattias ,  WUNDERLE, Philip, Justus

IPC: B01D59/42 ,  C25B11/02 ,  G01N27/447 ,  G01N27/49

CPC classification number: G01N27/44791 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0421 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44786 ,  G01N27/453

Abstract: The present disclosure provides systems and methods for sample preparation, processing and analysis. Also provided in the present disclosure is a fully-integrated electrophoresis cartridge which has a small footprint and configured to removably engage with the system. An electrophoresis cartridge adapted to releasably engage with a cartridge interface of a system is provided. The electrophoresis cartridge comprises: an electrophoresis assembly including: (1) an anode sub-assembly comprising an anode, (2) a cathode sub-assembly comprising a cathode; and (3) at least one electrophoresis capillary having a first end and a second end, wherein the cathode and the anode are configured to provide a voltage gradient across the first end and the second end of the at least one electrophoresis capillary.

Abstract translation: 本公开提供了用于样品制备，处理和分析的系统和方法。 本公开还提供了一种完全集成的电泳盒，其具有小的占地面积并且被配置为可移除地与系统接合。 提供了适于与系统的盒接口可释放地接合的电泳盒。 电泳盒包括：电泳组件，包括：（1）包括阳极的阳极子组件，（2）包括阴极的阴极子组件; 和（3）具有第一端和第二端的至少一个电泳毛细管，其中所述阴极和所述阳极被配置为在所述至少一个电泳毛细管的所述第一端和所述第二端提供电压梯度。

公开(公告)号：WO2015127422A1

公开(公告)日：2015-08-27

申请号：PCT/US2015/017231

申请日：2015-02-24

Applicant: HILL, Kevin

Inventor： HILL, Kevin

IPC: G01N27/416 ,  G01N27/447

CPC classification number: G01N27/44795 ,  B32B37/1284 ,  B32B37/18 ,  B32B41/00 ,  G01N27/44704 ,  G01N27/44721 ,  G01N27/44791 ,  Y10T156/10

Abstract: Some embodiments described herein relate to a capillary cartridge. A capillary cartridge can include a multiple of capillaries configured to be used for capillary electrophoresis. The capillaries can be fixed relative to each other in at least a radial direction by a capillary spacer plate. A slit plate can be coupled to the capillary spacer plate and can define optical access to the capillaries such that optical measurements, such as absorbance or fluorescence measurements can be made while the capillaries are within the cartridge.

Abstract translation: 这里描述的一些实施例涉及毛细管柱。 毛细管筒可以包括多个毛细管，其配置用于毛细管电泳。 毛细管可以通过毛细管间隔板在至少径向方向上相对于彼此固定。 狭缝板可以连接到毛细管间隔板并且可以限定对毛细管的光学接近，使得可以在毛细管在药筒内时进行光学测量，例如吸光度或荧光测量。

公开(公告)号：WO2015033090A1

公开(公告)日：2015-03-12

申请号：PCT/GB2014/000347

申请日：2014-09-03

Applicant: IZON SCIENCE LIMITED ,  GLOSSOP, Benjamin, Mark

Inventor： GLOSSOP, Benjamin, Mark ,  VOGEL, Robert ,  WEATHERALL, Eva ,  JONES, Martin, David

IPC: G01N15/12 ,  G01N15/00 ,  G01R29/24 ,  G01N15/10 ,  G01N33/487

CPC classification number: G01N27/44791 ,  G01N15/12 ,  G01N15/1209 ,  G01N15/1218 ,  G01N27/4163 ,  G01N27/44704 ,  G01N2015/0003 ,  G01N2015/1075 ,  G01R29/24

Abstract: A method of determining a charge of at least one test particle (as herein defined), comprising: applying one of an electric current or a voltage across an aperture connecting two chambers, whereby the chambers are at least partially filled with electrolyte and whereby the at least one test particle is suspended in the electrolyte of at least one of the chambers; measuring a value indicative of the other of the electric current or voltage across the aperture; determining a time interval between a first and a second point in time, the second point in time corresponding to a point in time when the measured current or voltage has reached a specific proportion of the measured current or voltage at the first point in time; and determining the charge of the at least one test particle by: determining a value indicative of an electrical velocity component of a total velocity of at least one calibration particle having a known charge, taking into account that the total velocity of the at least one calibration particle comprises a non¬ zero convective velocity component and the electrical velocity component; determining a value indicative of an electrical velocity component of a total velocity of the at least one test particle, taking into account that the total velocity of the at least one test particle comprises a non-zero convective velocity component and the electrical velocity component; and using the determined values indicative of the electrical velocity components of the test particle and the calibration particle to calibrate the quantitative relationship between the charge of the at least one test particle and the determined time interval.

Abstract translation: 一种确定至少一个测试颗粒（如本文定义的）的电荷的方法，包括：施加电流或电压中的一个穿过连接两个室的孔，由此所述室至少部分地填充有电解质， 至少一个测试颗粒悬浮在至少一个室的电解质中; 测量表示所述孔径上的另一电流或电压的值; 确定第一和第二时间点之间的时间间隔，所述第二时间点对应于当所测量的电流或电压达到所述第一时间点的测量电流或电压的特定比例的时间点; 以及通过以下步骤确定所述至少一个测试颗粒的电荷：考虑到所述至少一个校准的总速度，确定指示具有已知电荷的至少一个校准颗粒的总速度的电速分量的值 颗粒包括非零对流速度分量和电速分量; 考虑到至少一个测试颗粒的总速度包括非零对流速度分量和电速度分量，确定指示所述至少一个测试颗粒的总速度的电速分量的值; 并且使用所述确定的值来指示所述测试颗粒和所述校准颗粒的电速分量，以校准所述至少一个测试颗粒的电荷与所确定的时间间隔之间的定量关系。

公开(公告)号：WO2015028886A2

公开(公告)日：2015-03-05

申请号：PCT/IB2014/002143

申请日：2014-08-26

Applicant: QUANTUM BIOSYSTEMS INC.

Inventor： IKEDA, Shuji ,  OLDHAM, Mark ,  NORDMAN, Eric

IPC: G01N27/00

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  C23C14/0641 ,  C23C14/5886 ,  C23C16/06 ,  C23C16/345 ,  C23C16/56 ,  G01N27/3278 ,  G01N27/44704 ,  G01N27/44791

Abstract: The present disclosure provides methods for forming a nano-gap electrode. In some cases, a nano-gap having a width adjusted by a film thickness of a sidewall may be formed between a first electrode-forming part and a second electrode-forming part using sidewall which has contact with first electrode-forming part as a mask. Surfaces of the first electrode- forming part, the sidewall and the second electrode-forming part may then be exposed. The sidewall may then be removed to form a nano-gap between the first electrode-forming part and the second electrode-forming part.

Abstract translation: 本公开提供了用于形成纳米间隙电极的方法。 在一些情况下，可以使用与第一电极形成部分接触的侧壁作为掩模，在第一电极形成部分和第二电极形成部分之间形成宽度由侧壁的膜厚度调整的纳米间隙 。 然后可以暴露第一电极形成部分，侧壁和第二电极形成部分的表面。 然后可去除侧壁以在第一电极形成部分和第二电极形成部分之间形成纳米间隙。

公开(公告)号：WO2014205953A1

公开(公告)日：2014-12-31

申请号：PCT/CN2013/084948

申请日：2013-10-10

Applicant: 上海交通大学

Inventor： 曹成喜 ,  颜健 ,  孔凡志 ,  杨成章 ,  樊柳荫

IPC: B01D57/02

CPC classification number: G01N27/44704 ,  G01N27/44769 ,  G01N30/0005

Abstract: 一种生物化工技术领域的自由流电泳分离腔排气装置及其实施方法，排气装置包括充气筒、软管夹、橡皮软管、气液缓冲分离装置、自平衡回收装置、分离腔外壳、分离腔、废液回收瓶和回收三通阀。利用气液缓冲分离装置的储液功能，首先将大于分离腔体积十倍以上的缓冲液储积在气液缓冲分离装置中，然后通过手动充气筒，使气液缓冲分离装置内储积的缓冲液迅速进入分离腔，由于缓冲液的速度较大，能够很快充满分离腔，从而将分离腔内的空气排净。装置能够在非常短的时间内，用非常简单的操作将分离腔内的空气排出，解决了以往分离腔排气繁琐、困难的问题。装置有结构简单、成本低廉、易于操作、实用有效等优点。

公开(公告)号：WO2014165318A3

公开(公告)日：2014-12-31

申请号：PCT/US2014030991

申请日：2014-03-18

Applicant: IBM

Inventor： WALKER GEORGE F

IPC: B01D35/06

CPC classification number: G01N27/44704 ,  G01N27/44747

Abstract: A system of controlled translocation of macromolecules by gel electrophesis employs a funnel nanopore structure. A graphene portion is attached to a porous material layer including funnel-shaped pores such that the graphene portion blocks the side of the porous material layer having openings for smaller pores. A pair of electrical contacts is formed on the graphene portion. A dielectric material layer may be deposited to hold the graphene portion in place. A nanoscale hole is formed through the dielectric material layer and the graphene portion to provide a smallest opening in a funnel nanopore structure. The funnel nanopore structure is placed within a capsule configured for gel electrophoresis. A linear chain of molecules can pass through a funnel-shaped pore and the nanoscale hole during the gel electrophoresis. A graphene nanopore detector allows measurement of blockage current for sufficient resolution of base pairs in DNA's.

Abstract translation: 通过凝胶电穿孔控制大分子移位的系统采用漏斗状纳米孔结构。 石墨烯部分连接到包括漏斗形孔的多孔材料层，使得石墨烯部分阻挡具有用于较小孔的开口的多孔材料层的侧面。 在石墨烯部分上形成一对电触点。 可以沉积介电材料层以将石墨烯部分保持在适当位置。 通过介电材料层和石墨烯部分形成纳米尺度的孔，以在漏斗状纳米孔结构中提供最小的开口。 将漏斗状纳米孔结构置于配置用于凝胶电泳的胶囊内。 分子的线性链可以在凝胶电泳期间通过漏斗形孔和纳米级孔。 石墨烯纳米孔检测器允许测量阻塞电流以在DNA中的碱基对的足够分辨率。

公开(公告)号：WO2014138253A1

公开(公告)日：2014-09-12

申请号：PCT/US2014/020789

申请日：2014-03-05

Applicant: ARIZONA BOARD OF REGENTS, ACTING FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： ZHANG, Peiming ,  LINDSAY, Stuart

IPC: G01N27/26 ,  G01N33/68 ,  G06F19/22

CPC classification number: G01N33/48721 ,  C12Q1/37 ,  G01N15/12 ,  G01N27/44704 ,  G01N27/44747 ,  G01N27/44756 ,  G01N33/5438 ,  G01N33/6803 ,  G01N33/6848 ,  G01N2333/952 ,  G01N2570/00 ,  Y10T436/255

Abstract: Embodiments of the present disclosure are directed to methods, systems and devices, for analyzing the molecules. For example, in some embodiments, a system is provided which includes a first volume of conducting fluid, a second volume of conducting fluid, an orifice in communication with said first and second volumes of fluid, and means for applying an electric potential difference between said first and second volumes of fluid. In some such embodiments, a conjugate product is provided which comprises charged polymers each having attached thereto at least one first molecule for analysis, where the product carries a predetermined charge greater than the charge on the first molecule, and upon dissolving a product in the first volume of fluid, the product is directed into the orifice.

Abstract translation: 本公开的实施方案涉及用于分析分子的方法，系统和装置。 例如，在一些实施例中，提供了一种系统，其包括第一体积的导电流体，第二体积的导电流体，与所述第一和第二体积流体连通的孔，以及用于在所述第一和第二体积的流体之间施加电位差的装置 第一和第二体积的流体。 在一些这样的实施方案中，提供了缀合物产物，其包含各自带有至少一个用于分析的第一分子的带电聚合物，其中产物携带大于第一分子上的电荷的预定电荷，并且在将产物溶解在第一分子中 流体体积，产品被引导到孔中。