POLYVINYLALCOHOL COATED CAPILLARY ELECTROPHORESIS COLUMNS
    4.
    发明申请
    POLYVINYLALCOHOL COATED CAPILLARY ELECTROPHORESIS COLUMNS 审中-公开
    聚乙烯醇包衣毛细管电泳柱

    公开(公告)号:WO1996023221A1

    公开(公告)日:1996-08-01

    申请号:PCT/US1996001347

    申请日:1996-01-23

    IPC分类号: G01N27/477

    CPC分类号: G01N27/44704 G01N27/44752

    摘要: Coated capillary electrophoresis columns and methods for their use in electrophoretic separations are disclosed. The coated capillary columns include a length of tubing having an interior surface having an interconnected polymeric coating. The interconnected polymeric coating includes a hydrophobic hydrocarbon polymeric functionality covalently bound to the interior surface and polyvinylalcohol interconnected with the hydrophobic polymeric functionality. Exemplary columns are prepared by causing a Si-OH reactive compound, having a hydrophobic polymeric functionality, to react with Si-OH functionalities on the interior surface of capillary columns. Then causing vinyl acetate to polymerize in contact with the hydrophobic polymeric functionality and hydrolyzing the resulting polyvinylacetate forms a coating of interconnecting hydrophilic and hydrophobic polymers. Alternative exemplary coatings include a modified polyvinylalcohol such as a polyvinylether.

    摘要翻译: 公开了涂布毛细管电泳柱及其用于电泳分离的方法。 涂覆的毛细管柱包括具有内表面的一段长度的管,其具有互连的聚合物涂层。 相互连接的聚合物涂层包括与内表面共价结合的疏水性烃聚合物官能团和与疏水性聚合物官能团互连的聚乙烯醇。 通过使具有疏水聚合官能度的Si-OH反应性化合物与毛细管柱内表面上的Si-OH官能团反应来制备示例性柱。 然后使乙酸乙烯酯与疏水聚合官能团接触聚合并水解所得聚乙酸乙烯酯形成互连的亲水和疏水聚合物的涂层。 替代的示例性涂层包括改性的聚乙烯醇,例如聚乙烯醚。