The invention provides a yeast extract with pork flavor and a production method thereof. The yeast extract with pork flavor is made from the following raw materials: pork, water, protease, yeast extract and minor ingredients. The invention also provides a production method of the yeast extract with pork flavor, which comprises the following steps: (1) pork pretreatment; (2) pork enzyme inactivation; (3) pork zymohydrolysis; (4) refinement treatment; and (5) thermal reaction. The yeast extract with pork flavor can be used in instant noodles, meat products and the like to effectively improve themouth feel, special pork flavor and nutritive value of the terminal products.