The invention discloses a method for extracting xanthohumol in hop residue by supercritical CO2 technology, and relates to hop technology. The method comprises the following steps: taking raffinate (the hop residue) after alpha acid is extracted from hop by supercritical CO2 as a main raw material, adopting the supercritical CO2 technology, taking ethanol or methanol as an entrainer, and obtaining coarse xanthohumol; performing concentration and spray drying to obtain a coarse product of xanthohumol; and purifying to obtain a high-purity xanthohumol product. The method utilizes the raffinate after alpha acid is extracted from hop by supercritical CO2, and belongs to resource recycling. The method has simple process; the obtained xanthohumol product has good characteristics of high activity and good dissolubility; and the technological process is environment-friendly.