The invention discloses a process for producing vitamin K2 based on flavobacterium. The process comprises the following steps: inoculating a secondary seed culture solution into a fermentation culture solution, meanwhile adding a surface active agent with certain concentration into the fermentation broth to promote vitamin K2 to leak from inside of cells to the outside of the cells, so as to remove the inhibiting effect of intracellular metabolites. Compared with the prior art, the process disclosed by the invention has the advantages that the process for producing vitamin K2 based on flavobacterium disclosed by the invention is used for improving the fermentation unit of the vitamin K2 by 200-260% and providing a feasible fermentation strategy for large-scale industrial fermentation of the vitamin K2.