The invention provides an oyster activated peptide-zinc chelate and a preparation method and application thereof. The preparation method for the oyster activated peptide-zinc chelate comprises the following steps: preparing a peptide solution from oyster activated peptide, and then performing chelating reaction on the peptide solution and a zinc source to prepare chelated reaction liquid; performing sedimentation on the chelated reaction liquid by an organic solvent to prepare the oyster activated peptide-zinc chelate. Particularly, the mass percentage of components with the molecular weight smaller than 1,000 Da in the oyster activated peptide is over 90 percent. The contents of proteins and peptides in the oyster activated peptide are high, and the molecular weight of the peptides is relatively lower; the oyster activated peptide is low in sensitization, low in bitterness score and good in taste; when the oyster activated peptide is chelated with zinc, the chelating rate is high; the prepared oyster activated peptide-zinc chelate is easy to absorb and use; zinc supplementation can be realized, and various physiological efficacies of the oyster activated peptide can be comprehensively enhanced; the oyster activated peptide-zinc chelate has a good application prospect.