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CN105524873A 一种产α-淀粉酶的菌株及其产生的酸性α-淀粉酶 有权

一种产α-淀粉酶的菌株及其产生的酸性α-淀粉酶
摘要：
一种产α-淀粉酶的菌株及其产生的酸性α-淀粉酶，其特征在于：该菌株的名称为勒克氏菌属（Leclercia sp.）菌株Z1-2-1，于2015年11月5日保藏于中国微生物菌种保藏管理委员会普通微生物中心，菌种保藏号为 CGMCC No. 11587。该菌株为自主分离自土壤并经紫外诱变育种筛选而得到。利用该Z1-2-1菌株发酵制备的酸性α-淀粉酶，耐受pH范围3.4-6.4，最适作用pH6.0，最适作用温度55℃，于80℃下仍有酶活，在Ca2+ 1 mmol/L时酶活最高，为未加Ca2+的124%，一定浓度的Ca2+对酶活有促进作用。
摘要（英）：

The invention relates to a bacterial strain capable of producing alpha-amylase and acid alpha-amylase generated by the bacterial strain. The bacterial strain is characterized in that the name of the bacterial strain is Z1-2-1 belonging to Leclercia sp., and the bacterial strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on Nov 5, 2015 with the preservation number of CGMCC No.11587. The bacterial strain is obtained after being separated from the soil independently, and being subjected to ultraviolet mutation breeding for screening. The acid alpha-amylase prepared by fermenting the Z1-2-1 bacterial strain has the pH value tolerance range of 3.4-6.4, the optimum action pH of 6.0, and the optimum action temperature of 55 DEG C, has enzyme activity even at 80 DEG C, and has the highest enzyme activity when the Ca<2+> is 1mmol/L, which is 124% of that when no Ca<2+> is added, and the Ca<2+> with certain concentration has a promotion effect for the enzyme activity.

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