公开(公告)号：US09677135B2

公开(公告)日：2017-06-13

申请号：US14109709

申请日：2013-12-17

Applicant: Gen-Probe Incorporated

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Michael M. Becker

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

公开(公告)号：US10415092B2

公开(公告)日：2019-09-17

申请号：US15417736

申请日：2017-01-27

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold ,  Michael M. Becker

IPC: C12Q1/68 ,  C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/6834 ,  C12Q1/6865

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

公开(公告)号：US11136622B2

公开(公告)日：2021-10-05

申请号：US16546749

申请日：2019-08-21

Applicant: GEN-PROBE INCORPORATED

Inventor： James D. Carlson ,  Steven T. Brentano

IPC: C12Q1/6865 ,  C12Q1/70

Abstract: Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed.

公开(公告)号：US10407723B2

公开(公告)日：2019-09-10

申请号：US15595353

申请日：2017-05-15

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold ,  Michael M. Becker

IPC: C12Q1/68 ,  C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/6834 ,  C12Q1/6865

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

公开(公告)号：US09399796B2

公开(公告)日：2016-07-26

申请号：US13956158

申请日：2013-07-31

Applicant: Gen-Probe Incorporated

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

IPC: C12Q1/68

CPC classification number: C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US10724085B2

公开(公告)日：2020-07-28

申请号：US15902819

申请日：2018-02-22

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

IPC: C12Q1/68 ,  C12Q1/6865 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6853

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US10392656B2

公开(公告)日：2019-08-27

申请号：US15277167

申请日：2016-09-27

Applicant: GEN-PROBE INCORPORATED

Inventor： James D. Carlson ,  Steven T. Brentano

IPC: C12Q1/6865 ,  C12Q1/70

Abstract: Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed.

公开(公告)号：US10119163B2

公开(公告)日：2018-11-06

申请号：US15058702

申请日：2016-03-02

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

IPC: C12Q1/6865 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6853

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US09469881B2

公开(公告)日：2016-10-18

申请号：US14031353

申请日：2013-09-19

Applicant: Gen-Probe Incorporated

Inventor： James D. Carlson ,  Steven T. Brentano

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/6865 ,  C12Q1/706 ,  C12Q2600/158 ,  C12Q2600/16 ,  Y02A50/451 ,  Y02A50/54

Abstract: Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed.