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公开(公告)号：USRE48909E1
公开(公告)日：2022-02-01
申请号：US16398161
申请日：2019-04-29
IPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/689 , C12Q1/6806 , C12Q1/6895 , C12N15/10
摘要： The present invention provides nucleic acid amplification systems and methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results.
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公开(公告)号：US11035012B2
公开(公告)日：2021-06-15
申请号：US15904232
申请日：2018-02-23
发明人： Reinhold Pollner , Mehrdad Majlessi , Susan Yamagata , Michael M. Becker , Mark Reynolds , Lyle Arnold
IPC分类号： C12Q1/68 , C07H21/00 , C12Q1/70 , C12Q1/6837
摘要： The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.
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公开(公告)号：US20180251862A1
公开(公告)日：2018-09-06
申请号：US15904232
申请日：2018-02-23
发明人： Reinhold Pollner , Mehrdad Majlessi , Susan Yamagata , Michael M. Becker , Mark Reynolds , Lyle Arnold
IPC分类号： C12Q1/70 , C12Q1/6837
CPC分类号： C12Q1/707 , C12Q1/6837 , C12Q1/703 , Y02A50/53 , Y02A50/54 , C12Q2525/161
摘要： The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.
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公开(公告)号：US09765403B2
公开(公告)日：2017-09-19
申请号：US14938630
申请日：2015-11-11
IPC分类号： C12Q1/68
摘要： Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing regions, promoter sequences, and/or binding moieties. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares.
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公开(公告)号：US12065693B2
公开(公告)日：2024-08-20
申请号：US17061552
申请日：2020-10-01
IPC分类号： C12Q1/6816 , C12Q1/6832 , C12Q1/6834 , C12Q1/686
CPC分类号： C12Q1/6816 , C12Q1/6832 , C12Q1/6834 , C12Q1/686 , C12Q1/6816 , C12Q2525/161 , C12Q2525/301 , C12Q1/6832 , C12Q2565/107 , C12Q2563/179 , C12Q2527/101 , C12Q1/6832 , C12Q2537/1373 , C12Q2525/301 , C12Q2525/161 , C12Q1/6834 , C12Q2565/107 , C12Q2563/179 , C12Q2527/101 , C12Q1/6834 , C12Q2537/1373 , C12Q2525/301 , C12Q2525/161
摘要： The present invention provides compositions, kits and methods for the selective hybridization and capture of a specific target nucleic acid. The specific target nucleic acid may be present in a heterogeneous mixture of nucleic acids. Selective hybridization and capture provides a target nucleic acid that is substantially free of non-target and/or contaminating nucleic acids. Target nucleic acids that have been selectively hybridized and captured using the current invention are then used in subsequent analysis, wherein the presence of non-target and/or contaminating nucleic acids that interfere with said subsequent analysis have been substantially reduced or eliminated, thereby providing improved analysis results. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in subsequent analysis, or present in the environment in which an assay is performed, are free of bacterial or other contaminating nucleic acids.
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公开(公告)号：US10829801B2
公开(公告)日：2020-11-10
申请号：US15708039
申请日：2017-09-18
IPC分类号： C12Q1/68 , C07H21/00 , C12Q1/6816 , C12Q1/6832 , C12Q1/6834 , C12Q1/686
摘要： The present invention provides compositions, kits and methods for the selective hybridization and capture of a specific target nucleic acid. The specific target nucleic acid may be present in a heterogeneous mixture of nucleic acids. Selective hybridization and capture provides a target nucleic acid that is substantially free of non-target and/or contaminating nucleic acids. Target nucleic acids that have been selectively hybridized and captured using the current invention are then used in subsequent analysis, wherein the presence of non-target and/or contaminating nucleic acids that interfere with said subsequent analysis have been substantially reduced or eliminated, thereby providing improved analysis results. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in subsequent analysis, or present in the environment in which an assay is performed, are free of bacterial or other contaminating nucleic acids.
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公开(公告)号：US10167500B2
公开(公告)日：2019-01-01
申请号：US15069645
申请日：2016-03-14
IPC分类号： C12P19/34 , C12Q1/6848 , C12N15/10 , C12Q1/689 , C12Q1/6895 , C12Q1/6806
摘要： The present invention provides nucleic acid amplification systems and methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results.
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公开(公告)号：US09677135B2
公开(公告)日：2017-06-13
申请号：US14109709
申请日：2013-12-17
发明人： Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Norman C. Nelson , Lyle J. Arnold, Jr. , Michael M. Becker
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q2525/155 , C12Q2525/186 , C12Q2525/197 , C12Q2531/143 , C12Q2565/543
摘要： Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
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公开(公告)号：US10689712B2
公开(公告)日：2020-06-23
申请号：US15699502
申请日：2017-09-08
摘要： Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing regions, promoter sequences, and/or binding moieties. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares.
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公开(公告)号：US10415092B2
公开(公告)日：2019-09-17
申请号：US15417736
申请日：2017-01-27
发明人： Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Norman C. Nelson , Lyle J. Arnold , Michael M. Becker
IPC分类号： C12Q1/68 , C12Q1/6881 , C12Q1/6844 , C12Q1/6834 , C12Q1/6865
摘要： Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
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