Invention Grant
- Patent Title: Recombinant Escherichia coli for high efficiency production of fructosylated chondroitin and method for making thereof
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Application No.: US15891564Application Date: 2018-02-08
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Publication No.: US10508279B2Publication Date: 2019-12-17
- Inventor: Jing Wu , Quan Zhang , Liming Liu , Xiulai Chen , Jia Liu , Qiuling Luo
- Applicant: Jing Wu , Quan Zhang , Liming Liu , Xiulai Chen , Jia Liu , Qiuling Luo
- Applicant Address: CN Wuxi, JS
- Assignee: Jiangnan University
- Current Assignee: Jiangnan University
- Current Assignee Address: CN Wuxi, JS
- Agent Lili Chen
- Priority: CN201710616371 20170726
- Main IPC: C12N1/20
- IPC: C12N1/20 ; C12N15/70 ; C12N9/10 ; C12N9/90 ; C12P19/24 ; C12P19/26
Abstract:
The present invention relates to the field of biotechnology engineering. It provides a recombinant Escherichia coli that can produce fructosylated chondroitin with high yields and the method for constructing the recombinant strain. The present invention discloses a method for constructing an expression plasmid pETM6R1-RBS-glmM-GGGS-glmS that contains a glmM and a glmS gene under regulation of ribosome binding sites. The recombinant plasmid was transformed into E. coli K4 to obtain a recombinant E. coli strain ZQ33 that can produce fructosylated chondroitin up to 3.99 g·L−1 by fed-batch fermentation in a 5-L fermentor, which was increased by 108.90% compared to that of the wild type strain.
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