公开(公告)号：US10508279B2

公开(公告)日：2019-12-17

申请号：US15891564

申请日：2018-02-08

申请人： Jing Wu ,  Quan Zhang ,  Liming Liu ,  Xiulai Chen ,  Jia Liu ,  Qiuling Luo

发明人： Jing Wu ,  Quan Zhang ,  Liming Liu ,  Xiulai Chen ,  Jia Liu ,  Qiuling Luo

IPC分类号： C12N1/20 ,  C12N15/70 ,  C12N9/10 ,  C12N9/90 ,  C12P19/24 ,  C12P19/26

摘要： The present invention relates to the field of biotechnology engineering. It provides a recombinant Escherichia coli that can produce fructosylated chondroitin with high yields and the method for constructing the recombinant strain. The present invention discloses a method for constructing an expression plasmid pETM6R1-RBS-glmM-GGGS-glmS that contains a glmM and a glmS gene under regulation of ribosome binding sites. The recombinant plasmid was transformed into E. coli K4 to obtain a recombinant E. coli strain ZQ33 that can produce fructosylated chondroitin up to 3.99 g·L−1 by fed-batch fermentation in a 5-L fermentor, which was increased by 108.90% compared to that of the wild type strain.

公开(公告)号：US20190032062A1

公开(公告)日：2019-01-31

申请号：US15891564

申请日：2018-02-08

申请人： Jing Wu ,  Quan Zhang ,  Liming Liu ,  Xiulai Chen ,  Jia Liu ,  Qiuling Luo

发明人： Jing Wu ,  Quan Zhang ,  Liming Liu ,  Xiulai Chen ,  Jia Liu ,  Qiuling Luo

IPC分类号： C12N15/70 ,  C12P19/26 ,  C12N9/90 ,  C12N9/10 ,  C12P19/24

CPC分类号： C12N15/70 ,  C12N9/1096 ,  C12N9/90 ,  C12P19/24 ,  C12P19/26 ,  C12Y504/0201

摘要： The present invention relates to the field of biotechnology engineering. It provides a recombinant Escherichia coli that can produce fructosylated chondroitin with high yields and the method for constructing the recombinant strain. The present invention discloses a method for constructing an expression plasmid pETM6R1-RBS-glmM-GGGS-glmS that contains a glmM and a glmS gene under regulation of ribosome binding sites. The recombinant plasmid was transformed into E. coli K4 to obtain a recombinant E. coli strain ZQ33 that can produce fructosylated chondroitin up to 3.99 g·L−1 by fed-batch fermentation in a 5-L fermentor, which was increased by 108.90% compared to that of the wild type strain.