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    NOVEL OXO-RNA COMPOSITIONS AND THE RELATED APPLICATIONS THEREOF
    1.
    发明申请
    NOVEL OXO-RNA COMPOSITIONS AND THE RELATED APPLICATIONS THEREOF 有权

    公开(公告)号：US20250019709A1

    公开(公告)日：2025-01-16

    申请号：US18795444

    申请日：2024-08-06

    Applicant: Shi-Lung LIN ,  Sam LIN ,  Wei-Chih HUNG ,  Hsien-Lin LIU ,  Yu-Jing WU

    Inventor： Shi-Lung LIN ,  Sam LIN ,  Wei-Chih HUNG ,  Hsien-Lin LIU ,  Yu-Jing WU

    IPC: C12N15/113

    Abstract: This invention relates to a novel modified RNA composition (called oxo-RNA) comprising at least an oxo-nucleotide (containing oxopurine) in its 3′-end region (e.g. 3′-tail, 3′-UTR). The oxo-nucleotide includes 8-hydroxyguanine/8-oxoguanine/7,8-dihydro-8-oxoguanine (or called oxo-G/oxo-dG) and 8-hydroxyadenine/8-oxoadenine (or called oxo-A/oxo-dA). Oxo-RNA can be a single-stranded RNA sequence or double-stranded duplex, or even an RNA-DNA hybrid duplex. Advantageously, this new oxo-RNA composition not only greatly enhance RNA/DNA stability and functionality but also can prevent TREX1-mediated degradation and the related non-specific immunity over-activation (e.g. cytokine storm). Most importantly, the constructs of oxo-RNA can be designed to mimic antisense RNA oligonucleotide (aRNA-ASO), small interfering RNA (siRNA), short hairpin RNA (shRNA), microRNA (miRNA) mimic, microRNA precursor (pre-miRNA), double-stranded RNA (dsRNA), RNA-DNA hybrid, long noncoding RNA (IncRNA), small activating RNA (saRNA), messenger RNA (mRNA), and/or self-amplifying RNA/mRNA (saRNA/samRNA), or a combination thereof.

    DNA Polymerase Mutants with Increased Processivity of DNA Synthesis
    2.
    发明申请
    DNA Polymerase Mutants with Increased Processivity of DNA Synthesis 审中-公开

    公开(公告)号：US20190203190A1

    公开(公告)日：2019-07-04

    申请号：US16198773

    申请日：2018-11-22

    Applicant: Jing Wu ,  Li Wang

    Inventor： Jing Wu ,  Li Wang

    IPC: C12N9/12 ,  C12N15/52

    Abstract: Disclosed are Dpo4 DNA polymerase mutants with increased DNA synthesis processivity. The mutant enzymes, Dpo4 A181D and Dpo4 E63K, are constructed based on the wild-type Dpo4 derived from Sulfolobus solfataricus to obtain Dpo4DNA polymerase mutants with increased DNA processivity. The extension length of Dpo4 A181D and Dpo4 E63K are respectively increased by 25% and 18.8% than that of the wild-type Dpo4. The fidelity of Dpo4 A181D and wild-type Dpo4 are similar, and the fidelity of Dpo4 E63K is increased from that of the wild-type Dpo4. In summary, the mutants Dpo4 A181D and Dpo4 E63K obtained by the present invention have increased processivity compared to that of the wild-type Dpo4 DNA polymerase.

    Methods for deinking wastepaper by combined use of cutinase and chemical reagents
    3.
    发明授权
    Methods for deinking wastepaper by combined use of cutinase and chemical reagents 有权

    公开(公告)号：US09963824B2

    公开(公告)日：2018-05-08

    申请号：US15051677

    申请日：2016-02-24

    Applicant: Jing Wu ,  Lingqia Su ,  Ruoyu Hong

    Inventor： Jing Wu ,  Lingqia Su ,  Ruoyu Hong

    IPC: D21C9/02 ,  D21C5/02 ,  D21C5/00

    CPC classification number: D21C5/027 ,  D21C5/005 ,  Y02W30/648

    Abstract: The present invention provides methods for deinking wastepaper by combined use of cutinase and chemical reagents, which relates to the field of enzyme engineering. The method comprises the following steps: pulp preparation, enzymatic hydrolysis, pulp washing and dewatering, and flotation. The enzyme for enzymatic hydrolysis is cutinase at a concentration of 10-20 U/g absolute dry pulp; and 0.5-4% Na2SiO3, 0.1-0.8% MgSO4, 0.1-0.8% EDTA, 0.1-4% H2O2 are used in the chemical treatment. With combined use of enzymatic and chemical treatment, the present invention has solved the problem of the current enzymatic method for deinking that requires large dosage of enzymes and thus high cost. Through proper choice of the kind and the amount of chemical reagents, synergistic effects of the enzymatic and chemical treatment can be achieved, thus increasing the effectiveness of the deinking process. In addition, this method does not require alkaline chemicals, which alleviates the problem of sewage treatment associated with conventional deinking methods.

    Antenna Module
    4.
    发明申请
    Antenna Module 审中-公开

    公开(公告)号：US20170346161A1

    公开(公告)日：2017-11-30

    申请号：US15417118

    申请日：2017-01-26

    Applicant: Jing Wu

    Inventor： Jing Wu

    IPC: H01Q1/24 ,  H01Q1/48

    CPC classification number: H01Q1/243 ,  H01Q1/24 ,  H01Q1/241 ,  H01Q1/48 ,  H01Q1/50 ,  H01Q5/328 ,  H01Q21/28

    Abstract: The present disclosure provides an antenna module, including a metal housing having accommodating space and a circuit board accommodated in the accommodating space, the metal housing includes a metal back cover and a metal side wall, the metal side wall includes a side wall main body and a first radiator extending from an end of the side wall main body, a second radiator extending from another end of the side wall main body which is spaced with and arranged opposite to the first radiator, and a third radiator provided between the first radiator and the second radiator, a headroom region is formed between the third radiator and the circuit board. The present disclosure provides an antenna module having frequency bands of wireless signal and a good overall appearance.

    Cutinase-Producing Genetically Engineered Microorganism and Use Thereof
    6.
    发明申请
    Cutinase-Producing Genetically Engineered Microorganism and Use Thereof 有权
    角质酶生产基因工程微生物及其应用

    公开(公告)号：US20120149086A1

    公开(公告)日：2012-06-14

    申请号：US13264560

    申请日：2011-02-28

    Applicant: Jian Chen ,  Jing Wu ,  Dan Wu ,  Lei Wang

    Inventor： Jian Chen ,  Jing Wu ,  Dan Wu ,  Lei Wang

    IPC: C12N9/18 ,  C12N1/21

    CPC classification number: C12N9/18 ,  C12Y301/01074

    Abstract: The present invention relates to the field of bioengineering and discloses a cutinase-producing genetically engineered microorganism and use thereof. Recombinant plasmid Tfu_0883-hlyAs/pET20b(+) was constructed and transformed into E. coli BL21(DE3) to obtain recombinant E. coli strain Tfu_0883-hlyAs/pET20b(+)/E. coli BL21(DE3). Specific growth rate was maintained at a certain value using fed-batch fermentation mode. After fermenting 30-34 hours, the enzyme activity in the supernatant reached 700-750 U/mL. The present invention uses glycerol as the main raw material and employs semi-synthetic medium, has the advantages of good stability and ease of control, and is suitable for large-scale production.

    Abstract translation: 本发明涉及生物工程领域，并公开了一种生产基因工程转基因的微生物及其用途。 构建重组质粒Tfu_0883-hlyAs / pET20b（+），转化大肠杆菌BL21（DE3），得到重组大肠杆菌菌株Tfu_0883-hlyAs / pET20b（+）/ E。 大肠杆菌BL21（DE3）。 使用补料分批发酵模式，比生长速率保持在一定值。 发酵30-34小时后，上清液中酶活力达到700〜750U / mL。 本发明以甘油为主要原料，采用半合成培养基，具有稳定性好，易控制的优点，适合大规模生产。

    Recovery from control plane failures in the LDP signalling protocol
    7.
    发明授权
    Recovery from control plane failures in the LDP signalling protocol 失效
    从LDP信令协议中的控制面故障恢复

    公开(公告)号：US07881183B2

    公开(公告)日：2011-02-01

    申请号：US11467953

    申请日：2006-08-29

    Applicant: Jing Wu ,  Michel Savoie ,  Hussein Mouftah

    Inventor： Jing Wu ,  Michel Savoie ,  Hussein Mouftah

    IPC: H04J1/16

    CPC classification number: H04J3/14 ,  H04L45/02 ,  H04L45/28 ,  H04L45/507 ,  H04L45/64

    Abstract: The invention provides a distributed back-up mechanism and a two-step method for facilitating fast control plane recovery in a switched network. In a preferred embodiment, a Label Information Database (LID) maintained at a control node of a GMPLS network is mirrored to an upstream node using the Label Distribution Protocol (LDP). After a control plane interruption resulting in the LDP restart, the control node, using the mirrored information at the upstream node, conducts first a fast coarse LID recovery wherein only the idle labels are identified, to enable the restarted LDP session to process new connection setup. A detailed LDP state information recovery performs in the background in parallel to the normal LDP operations, e.g. using on-demand LDP queries.

    Abstract translation: 本发明提供一种用于促进交换网络中的快速控制平面恢复的分布式备份机制和两步法。 在优选实施例中，使用标签分发协议（LDP）将维护在GMPLS网络的控制节点处的标签信息数据库（LID）镜像到上游节点。 在导致LDP重新启动的控制平面中断之后，使用上游节点处的镜像信息的控制节点首先进行快速粗略LID恢复，其中仅标识空闲标签，以使得重启的LDP会话能够处理新的连接设置 。 详细的LDP状态信息恢复在背景中与正常的LDP操作并行执行，例如， 使用按需LDP查询。

    Electronic junction devices featuring redox electrodes
    8.
    发明授权
    Electronic junction devices featuring redox electrodes 有权
    具有氧化还原电极的电子连接装置

    公开(公告)号：US07737433B2

    公开(公告)日：2010-06-15

    申请号：US11536031

    申请日：2006-09-28

    Applicant: Richard L. McCreery ,  Kenneth J. Mobley ,  Jing Wu

    Inventor： Richard L. McCreery ,  Kenneth J. Mobley ,  Jing Wu

    IPC: H01L35/24 ,  H01L51/00

    CPC classification number: H01L51/0052 ,  B82Y10/00 ,  H01L51/0075 ,  H01L51/0595 ,  H01L51/4226 ,  Y10T428/261 ,  Y10T428/31504 ,  Y10T428/31678

    Abstract: The electronic properties of molecular junctions of the general type carbon/molecule/TiO2/Au as examples of “molecular heterojunctions” consisting of a molecular monolayer and a semiconducting oxide. Junctions containing fluorene bonded to pyrolyzed photoresist film (PPF) were compared to those containing Al2O3 instead of fluorene, and those with only the TiO2 layer. The responses to voltage sweep and pulse stimulation were strongly dependent on junction composition and temperature. A transient current response lasting a few milliseconds results from injection and trapping of electrons in the TiO2 layer, and occurred in all three junction types studied. Conduction in PPF/TiO2/Au junctions is consistent with space charge limited conduction at low voltage, then a sharp increase in current once the space charge fills all the traps. With fluorene present, there is a slower, persistent change in junction conductance which may be removed by a reverse polarity pulse. This “memory” effect is attributed to a redox process in the TiO2 which generates TiIII and/or TiII, which have much higher conductance than TiO2 due to the presence of conduction band electrons. The redox process amounts to “dynamic doping” of the TiO2 layer by imposed electric field. The memory effect arises from a combination of the properties of the molecular and oxide layers, and is a special property of the molecular heterojunction configuration.

    Abstract translation: 作为分子单层和半导体氧化物组成的“分子异质结”的一般类型的碳/分子/ TiO 2 / Au的分子结的电子性质。 将与热解的光致抗蚀剂膜（PPF）结合的芴的接合物与含有Al 2 O 3而不是芴的那些进行比较，仅与TiO 2层接合。 对电压扫描和脉冲刺激的响应强烈依赖于结的组成和温度。 持续几毫秒的瞬态电流响应来自TiO 2层中的电子的注入和捕获，并且发生在研究的所有三种连接类型中。 PPF / TiO2 / Au结中的传导与低电压下的空间电荷限制传导一致，一旦空间电荷填充所有陷阱，电流就会急剧增加。 当存在芴时，会导致电阻较慢，持续的变化，这可能会被反极性脉冲除去。 这种“记忆”效应归因于TiO 2中的氧化还原过程，其产生TiIII和/或TiII，其由于存在导带电子而具有比TiO 2高得多的电导。 氧化还原过程相当于施加电场对TiO 2层的“动态掺杂”。 记忆效应是由分子和氧化物层的性质的组合产生的，并且是分子异质结构型的特殊性质。

    Method for asymmetric hydrosilylation of ketones
    9.
    发明授权
    Method for asymmetric hydrosilylation of ketones 有权
    酮的不对称氢化硅烷化方法

    公开(公告)号：US07718831B2

    公开(公告)日：2010-05-18

    申请号：US11816139

    申请日：2006-02-16

    Applicant: Albert S Chan ,  Jianxin Ji ,  Jing Wu

    Inventor： Albert S Chan ,  Jianxin Ji ,  Jing Wu

    IPC: C07C29/143 ,  B01J31/24 ,  B01J31/18

    CPC classification number: C07C29/143 ,  B01J31/1815 ,  B01J31/2409 ,  B01J2231/341 ,  B01J2531/0266 ,  B01J2531/16 ,  C07B2200/07 ,  C07C33/18 ,  C07C33/22 ,  C07C33/24 ,  C07C33/46

    Abstract: Method of asymmetrically hydrosilylating substrates using catalysts having a ligand of the compound of the formula (I) wherein R is optionally substituted alkyl, cycloalkyl, aryl or heteroaryl; R′ is hydrogen, optionally substituted lower alkyl; and R″ is hydrogen, halogen, optionally substituted alkyl, hydroxy, amino (e.g., primary, secondary or tertiary), alkenyl; or an enantiomer thereof; or an enantiomeric mixture thereof with a transition metal. Particularly suitable reactions include the asymmetric hydrosilylation of ketones.

    Abstract translation: 使用具有式（I）化合物的配体的催化剂的不对称氢化硅烷化方法，其中R是任选取代的烷基，环烷基，芳基或杂芳基; R'是氢，任选取代的低级烷基; 和R“是氢，卤素，任选取代的烷基，羟基，氨基（例如伯，仲或叔），烯基; 或其对映体; 或其与过渡金属的对映体混合物。 特别合适的反应包括酮的不对称氢化硅烷化。

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