Invention Grant
- Patent Title: Amplification reporter with base-pairing oligomers
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Application No.: US16834854Application Date: 2020-03-30
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Publication No.: US11085070B2Publication Date: 2021-08-10
- Inventor: John F. Regan , Dianna Maar , Dawne N. Shelton , Tina C. Legler , Samantha Cooper , Wei Yang , Eli A. Hefner , Niels Klitgord
- Applicant: Bio-Rad Laboratories, Inc.
- Applicant Address: US CA Hercules
- Assignee: Bio-Rad Laboratories, Inc.
- Current Assignee: Bio-Rad Laboratories, Inc.
- Current Assignee Address: US CA Hercules
- Agency: Kolisch Hartwell, P.C.
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/6818 ; C12Q1/6823 ; C12Q1/6848

Abstract:
System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
Public/Granted literature
- US20200270677A1 AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS Public/Granted day:2020-08-27
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