Invention Grant
- Patent Title: Sample indexing for single cells
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Application No.: US16848241Application Date: 2020-04-14
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Publication No.: US12084712B2Publication Date: 2024-09-10
- Inventor: Christina Chang , Christina Fan , Eleen Shum , Jody Martin , Nidhanjali Bansal , James Ghadiali , Katherine Lazaruk , Gretchen Yinbon Lam
- Applicant: BECTON, DICKINSON AND COMPANY
- Applicant Address: US NJ Franklin Lakes
- Assignee: Becton, Dickinson and Company
- Current Assignee: Becton, Dickinson and Company
- Current Assignee Address: US NJ Franklin Lakes
- Agency: Sheppard, Mullin, Richter & Hampton LLP
- Main IPC: C12Q1/6804
- IPC: C12Q1/6804 ; C12Q1/6806 ; C12Q1/6809 ; C12Q1/6811 ; C12Q1/6816 ; C12Q1/6855 ; C12Q1/6865 ; C12Q1/6869 ; C12Q1/6876 ; G01N33/532 ; B82Y5/00 ; C12Q1/6834 ; C12Q1/6837

Abstract:
Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.
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