公开(公告)号：US20220187286A1

公开(公告)日：2022-06-16

申请号：US17151050

申请日：2021-01-15

Applicant: Becton, Dickinson and Company

Inventor： Hye-Won Song ,  Jody Martin

IPC: G01N33/543 ,  G01N33/68 ,  G01N33/74 ,  G01N33/563

Abstract: Systems, methods, compositions, and kits for measuring secreted factors from cells are disclosed herein, including those capable of determining single cell secretion activity and protein expression and/or gene expression simultaneously. Disclosed herein include solid supports comprising a plurality of capture probes capable of specifically binding to at least one of the plurality of secreted factors secreted by a single cell. Also disclosed herein include secreted factor-binding reagents capable of specifically binding to a secreted factor bound by a capture probe. A secreted factor-binding reagent can comprise a secreted factor-binding reagent specific oligonucleotide comprising a unique factor identifier sequence for the secreted factor-binding reagent.

公开(公告)号：US11315661B2

公开(公告)日：2022-04-26

申请号：US15886763

申请日：2018-02-01

Applicant: Becton, Dickinson and Company

Inventor： Jody Martin ,  Adam Wright ,  Paul Waterman ,  James Ghadiali

IPC: G16B50/00 ,  G06F16/248 ,  G06F16/2455 ,  G16B30/00 ,  G16B50/30

Abstract: Aspects of the present disclosure include systems for use in preparing an epitope tagged biomolecule reagent. A reagent preparatory apparatus for preparing the epitope tagged biomolecule reagent from an activated biomolecule and activated epitope tag is also described. Methods for communicating and receiving an epitope tagged biomolecule reagent request and preparing the subject epitope tagged biomolecule reagents are also provided.

公开(公告)号：US10472521B2

公开(公告)日：2019-11-12

申请号：US15371064

申请日：2016-12-06

Applicant: Becton, Dickinson and Company

Inventor： Robert J. Radford ,  Douglas B. Sherman ,  Jody Martin ,  James Ghadiali

IPC: C09B69/10 ,  G01N33/58

Abstract: Photostable polymeric tandem dyes are provided. The photostable polymeric tandem dyes include a water soluble light harvesting multichromophore and a luminescent metal complex covalently linked to the multichromophore and in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.

公开(公告)号：US20250066843A1

公开(公告)日：2025-02-27

申请号：US18728980

申请日：2023-02-06

Applicant: Becton, Dickinson and Company

Inventor： Jody Martin ,  Katherine Lazaruk ,  Tracy Campbell ,  Dennis Prosen ,  Adam Thomas Wright ,  Hye-Won Song

IPC: C12Q1/6851 ,  C12Q1/6804 ,  C12Q1/6834

Abstract: Disclosed herein include systems, methods, compositions, and kits for reducing non-specific noise-causing oligonucleotides in library preparations. Capture particles present in PCR reactions that are derived from partitions that did not contain a cell (e.g., noise capture particles) provide a significant source of non-specific nucleic acid and noise in single cell multiomics library preparations. In some embodiments of the methods provided herein, said noise capture particles are removed by a variety of approaches.

公开(公告)号：US20240369545A1

公开(公告)日：2024-11-07

申请号：US18686824

申请日：2022-08-30

Applicant: Becton, Dickinson and Company

Inventor： Adam Thomas Wright ,  Jody Martin ,  Hye-Won Song ,  Samatha Vadrevu

IPC: G01N33/53 ,  G01N33/543 ,  G01N33/58 ,  G01N33/68

Abstract: Antibodies attached to oligonucleotides can bind to proteins of cells. These oligonucleotides can be released and captured by solid support oligonucleotides attached to solid supports. A detection oligonucleotide can hybridize to the captured oligonucleotides forming a sandwich of solid support oligonucleotide-captured oligonucleotide-detection oligonucleotide. The detection oligonucleotide and the captured oligonucleotides it binds to can be quantified by, for example, the fluorescent intensity of second detectable moieties (e.g., dye(s)) on the detection oligonucleotide. The identity of the protein quantified can be determined by, for example, the fluorescent intensity of first detectable moieties (e.g., dye(s)) on the solid support with the sandwich of solid support oligonucleotide-captured oligonucleotide-detection oligonucleotide.

公开(公告)号：US12084712B2

公开(公告)日：2024-09-10

申请号：US16848241

申请日：2020-04-14

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Christina Chang ,  Christina Fan ,  Eleen Shum ,  Jody Martin ,  Nidhanjali Bansal ,  James Ghadiali ,  Katherine Lazaruk ,  Gretchen Yinbon Lam

IPC: C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12Q1/6869 ,  C12Q1/6876 ,  G01N33/532 ,  B82Y5/00 ,  C12Q1/6834 ,  C12Q1/6837

CPC classification number: C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12Q1/6869 ,  C12Q1/6876 ,  G01N33/532 ,  B82Y5/00 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2563/149 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2600/16 ,  G01N2458/10 ,  C12Q1/6869 ,  C12Q2525/179 ,  C12Q2563/131 ,  C12Q2563/179 ,  C12Q1/6869 ,  C12Q2563/131 ,  C12Q2563/179

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.

公开(公告)号：US20180088112A1

公开(公告)日：2018-03-29

申请号：US15715028

申请日：2017-09-25

Applicant: Cellular Research, Inc. ,  Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  C12Q1/68 ,  G01N33/543 ,  C12N15/10

CPC classification number: G01N33/566 ,  B01J2219/00646 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07K2319/01 ,  C12N15/1006 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  G01N33/505 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/6842 ,  G01N33/6857 ,  G06F19/00 ,  G16B25/10 ,  G16B99/00 ,  G16H10/20 ,  G16H20/13 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US20230125113A1

公开(公告)日：2023-04-27

申请号：US17821449

申请日：2022-08-22

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  G16B99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/68

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US20210263019A1

公开(公告)日：2021-08-26

申请号：US17184405

申请日：2021-02-24

Applicant: Becton, Dickinson and Company

Inventor： Jody Martin ,  James Ghadiali ,  Mirko Corselli ,  Adam Wright ,  Aaron Jacob Tyznik

IPC: G01N33/53

Abstract: Disclosed herein include methods, compositions, and kits suitable for spectral unmixing and compensation in multi-parameter flow cytometry. Disclosed herein include reagents which comprise two antibodies conjugated to one another to form a bispecific reagent (e.g., a bispecific probe). A first antibody can have affinity for a highly expressed antigen on the surface of a cell of interest and a second antibody can have affinity for each of the antibody-dye conjugates in a multi-parameter panel. There are provided, in some embodiments, methods of using the bispecific reagent to determine spillover, perform compensation, and generate a compensation matrix.

公开(公告)号：US20210246492A1

公开(公告)日：2021-08-12

申请号：US17174249

申请日：2021-02-11

Applicant: Becton, Dickinson and Company

Inventor： Hye-Won Song ,  Jody Martin

IPC: C12Q1/6813 ,  C12N15/117

Abstract: Disclosed herein include systems, methods, compositions, and kits for performing intracellular AbSeq assays. There are provided, in some embodiments, methods for measuring intracellular target expression. The method can comprise fixing and permeabilizing a plurality of cells before contacting with a plurality of intracellular target-binding reagents capable of specifically binding to an intracellular target. Intracellular target-binding reagents can comprise an intracellular target-binding reagent specific oligonucleotide comprising a unique intracellular target identifier for the intracellular target-binding reagent specific oligonucleotide. The method can further comprise removing the permeabilizing agent and reversing the fixation.