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    USING RANDOM PRIMING TO OBTAIN FULL-LENGTH V(D)J INFORMATION FOR IMMUNE REPERTOIRE SEQUENCING
    1.
    发明申请
    USING RANDOM PRIMING TO OBTAIN FULL-LENGTH V(D)J INFORMATION FOR IMMUNE REPERTOIRE SEQUENCING 有权

    公开(公告)号：US20210139970A1

    公开(公告)日：2021-05-13

    申请号：US17091639

    申请日：2020-11-06

    Applicant: Becton, Dickinson and Company

    Inventor： Christina Chang ,  Margaret Nakamoto

    IPC: C12Q1/6865 ,  C12Q1/6867 ,  C12Q1/6874

    Abstract: Disclosed herein include systems, methods, compositions, and kits for labeling nucleic acid targets in a sample. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3′ end and are subsequently barcoded on the 5′ end following a template switching reaction and intermolecular and/or intramolecular hybridization and extension. 5′- and/or 3′-barcoded nucleic acid targets can serve as templates for amplification reactions and/or random priming and extension reactions to generate a sequencing library. The method can comprise generating a full-length sequence of the nucleic acid target by aligning a plurality of sequencing reads. Immune repertoire profiling methods are also provided in some embodiments.

    OLIGONUCLEOTIDE-COMPRISING CELLULAR COMPONENT-BINDING REAGENTS AND METHODS OF USING THE SAME
    2.
    发明申请
    OLIGONUCLEOTIDE-COMPRISING CELLULAR COMPONENT-BINDING REAGENTS AND METHODS OF USING THE SAME 审中-公开

    公开(公告)号：US20200248263A1

    公开(公告)日：2020-08-06

    申请号：US16748691

    申请日：2020-01-21

    Applicant: Becton, Dickinson and Company

    Inventor： Margaret Nakamoto ,  Eleen Shum ,  Nidhanjali BANSAL ,  Christina Chang

    IPC: C12Q1/6883 ,  G01N33/53

    Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).

    METHODS OF ASSOCIATING PHENOTYPICAL DATA AND SINGLE CELL SEQUENCING DATA
    6.
    发明申请
    METHODS OF ASSOCIATING PHENOTYPICAL DATA AND SINGLE CELL SEQUENCING DATA 审中-公开

    公开(公告)号：US20200332351A1

    公开(公告)日：2020-10-22

    申请号：US16849191

    申请日：2020-04-15

    Applicant: Becton, Dickinson and Company

    Inventor： Christina Chang ,  Sixing Li

    IPC: C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6858

    Abstract: Disclosed herein include systems, methods, compositions, and kits for associating single cell sequencing data with phenotypic data and/or agent exposure. In some embodiments, cell indexing oligonucleotides comprising cell indexing sequences are associated with cells. In some embodiments, synthetic particles are associated with stochastic labels comprising cell indexing sequences. Sequential hybridizing and imaging of probes annealing to a cell indexing sequence can generate a spatial index. The spatial index can be employed to identify of the partition from which a sequenced nucleic acid target molecule originated.

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