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1.
公开(公告)号:US20210139970A1
公开(公告)日:2021-05-13
申请号:US17091639
申请日:2020-11-06
Applicant: Becton, Dickinson and Company
Inventor: Christina Chang , Margaret Nakamoto
IPC: C12Q1/6865 , C12Q1/6867 , C12Q1/6874
Abstract: Disclosed herein include systems, methods, compositions, and kits for labeling nucleic acid targets in a sample. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3′ end and are subsequently barcoded on the 5′ end following a template switching reaction and intermolecular and/or intramolecular hybridization and extension. 5′- and/or 3′-barcoded nucleic acid targets can serve as templates for amplification reactions and/or random priming and extension reactions to generate a sequencing library. The method can comprise generating a full-length sequence of the nucleic acid target by aligning a plurality of sequencing reads. Immune repertoire profiling methods are also provided in some embodiments.
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2.
公开(公告)号:US20200248263A1
公开(公告)日:2020-08-06
申请号:US16748691
申请日:2020-01-21
Applicant: Becton, Dickinson and Company
Inventor: Margaret Nakamoto , Eleen Shum , Nidhanjali BANSAL , Christina Chang
IPC: C12Q1/6883 , G01N33/53
Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).
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公开(公告)号:US12084712B2
公开(公告)日:2024-09-10
申请号:US16848241
申请日:2020-04-14
Applicant: BECTON, DICKINSON AND COMPANY
Inventor: Christina Chang , Christina Fan , Eleen Shum , Jody Martin , Nidhanjali Bansal , James Ghadiali , Katherine Lazaruk , Gretchen Yinbon Lam
IPC: C12Q1/6804 , C12Q1/6806 , C12Q1/6809 , C12Q1/6811 , C12Q1/6816 , C12Q1/6855 , C12Q1/6865 , C12Q1/6869 , C12Q1/6876 , G01N33/532 , B82Y5/00 , C12Q1/6834 , C12Q1/6837
CPC classification number: C12Q1/6804 , C12Q1/6806 , C12Q1/6809 , C12Q1/6811 , C12Q1/6816 , C12Q1/6855 , C12Q1/6865 , C12Q1/6869 , C12Q1/6876 , G01N33/532 , B82Y5/00 , C12Q1/6834 , C12Q1/6837 , C12Q2563/149 , C12Q2563/179 , C12Q2563/185 , C12Q2600/16 , G01N2458/10 , C12Q1/6869 , C12Q2525/179 , C12Q2563/131 , C12Q2563/179 , C12Q1/6869 , C12Q2563/131 , C12Q2563/179
Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.
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4.
公开(公告)号:US11834715B2
公开(公告)日:2023-12-05
申请号:US16748691
申请日:2020-01-21
Applicant: Becton, Dickinson and Company
Inventor: Margaret Nakamoto , Eleen Shum , Nidhanjali Bansal , Christina Chang
IPC: C12Q1/6883 , G01N33/53
CPC classification number: C12Q1/6883 , G01N33/5308 , C12Q2600/106 , C12Q2600/118 , C12Q2600/158
Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).
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公开(公告)号:US20220315988A1
公开(公告)日:2022-10-06
申请号:US17830897
申请日:2022-06-02
Applicant: Becton, Dickinson and Company
Inventor: Katherine Lazaruk , Eleen Shum , Christina Chang
IPC: C12Q1/6806 , C12Q1/6851 , C12Q1/6876
Abstract: Disclosed herein include systems, methods, compositions, and kits for PCR normalization. In some embodiments, after barcoding copies of a higher abundance target (e.g., a cDNA species), the barcoded copies are amplified using a pair of forward primers comprising one or more mismatches and a reverse primer. The amplified copies can be further linearly amplified using a forward primer comprising the sequence of one of the pair of forward primers, and a reverse primer.
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公开(公告)号:US20200332351A1
公开(公告)日:2020-10-22
申请号:US16849191
申请日:2020-04-15
Applicant: Becton, Dickinson and Company
Inventor: Christina Chang , Sixing Li
IPC: C12Q1/6837 , C12Q1/6827 , C12Q1/6858
Abstract: Disclosed herein include systems, methods, compositions, and kits for associating single cell sequencing data with phenotypic data and/or agent exposure. In some embodiments, cell indexing oligonucleotides comprising cell indexing sequences are associated with cells. In some embodiments, synthetic particles are associated with stochastic labels comprising cell indexing sequences. Sequential hybridizing and imaging of probes annealing to a cell indexing sequence can generate a spatial index. The spatial index can be employed to identify of the partition from which a sequenced nucleic acid target molecule originated.
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7.
公开(公告)号:US20180088112A1
公开(公告)日:2018-03-29
申请号:US15715028
申请日:2017-09-25
Applicant: Cellular Research, Inc. , Becton, Dickinson and Company
Inventor: Christina Fan , Christina Chang , Eleen Shum , Olaf Zoellner , Nidhanjali Bansal , James Ghadiali , Gretchen Yinbon Lam , Eric Jensen , Jurg Rohrer , Brent Gaylord , Jody Martin , Jason G. Vidal
IPC: G01N33/566 , C12Q1/68 , G01N33/543 , C12N15/10
CPC classification number: G01N33/566 , B01J2219/00646 , B01J2219/00722 , B01J2219/00725 , C07K2319/01 , C12N15/1006 , C12N15/1096 , C12Q1/68 , C12Q1/6804 , C12Q1/6806 , C12Q1/6823 , C12Q1/6869 , G01N33/505 , G01N33/54306 , G01N33/54313 , G01N33/6842 , G01N33/6857 , G06F19/00 , G16B25/10 , G16B99/00 , G16H10/20 , G16H20/13 , C12Q2525/161 , C12Q2525/173 , C12Q2563/179 , C12Q2565/514
Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.
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8.
公开(公告)号:US11773436B2
公开(公告)日:2023-10-03
申请号:US17091639
申请日:2020-11-06
Applicant: Becton, Dickinson and Company
Inventor: Christina Chang , Margaret Nakamoto
IPC: C12Q1/6806 , C12Q1/6865 , C12Q1/6867 , C12Q1/6874
CPC classification number: C12Q1/6865 , C12Q1/6867 , C12Q1/6874 , C12Q2521/101 , C12Q2521/107 , C12Q2565/1025
Abstract: Disclosed herein include systems, methods, compositions, and kits for labeling nucleic acid targets in a sample. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3′ end and are subsequently barcoded on the 5′ end following a template switching reaction and intermolecular and/or intramolecular hybridization and extension. 5′- and/or 3′-barcoded nucleic acid targets can serve as templates for amplification reactions and/or random priming and extension reactions to generate a sequencing library. The method can comprise generating a full-length sequence of the nucleic acid target by aligning a plurality of sequencing reads. Immune repertoire profiling methods are also provided in some embodiments.
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公开(公告)号:US20230125113A1
公开(公告)日:2023-04-27
申请号:US17821449
申请日:2022-08-22
Applicant: Becton, Dickinson and Company
Inventor: Christina Fan , Christina Chang , Eleen Shum , Olaf Zoellner , Nidhanjali Bansal , James Ghadiali , Gretchen Yinbon Lam , Eric Jensen , Jurg Rohrer , Brent Gaylord , Jody Martin , Jason G. Vidal
IPC: G01N33/566 , G16B99/00 , G01N33/50 , G01N33/543 , G01N33/68 , C12Q1/6804 , G16Z99/00 , C12N15/10 , C12Q1/6806 , C12Q1/6823 , C12Q1/6869 , C12Q1/68
Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.
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公开(公告)号:US20200172957A1
公开(公告)日:2020-06-04
申请号:US16789311
申请日:2020-02-12
Applicant: BECTON, DICKINSON AND COMPANY
Inventor: Christina Chang , Christina Fan , Eleen Shum
IPC: C12Q1/6804 , C12Q1/6816 , C12Q1/6806 , C12Q1/6811 , C12Q1/6809 , C12Q1/6869 , C12Q1/6855 , C12Q1/6865 , C12Q1/6876 , G01N33/532
Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.
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