公开(公告)号：US20220162695A1

公开(公告)日：2022-05-26

申请号：US17531555

申请日：2021-11-19

Applicant: Becton, Dickinson and Company ,  Immudex Aps

Inventor： Cynthia Sakofsky ,  Katherine Lazaruk ,  Margaret Nakamoto ,  Devon Joseph Jensen ,  Kivin Dahl Jacobsen

IPC: C12Q1/6874 ,  C12Q1/6804

Abstract: Disclosed herein include systems, methods, compositions, and kits suitable for the use of dextramers in single cell analysis. In some embodiments, one or more primers allowing generation of separate libraries for proteins (e.g., antibodies) and for dextramers are used.

公开(公告)号：US20210355484A1

公开(公告)日：2021-11-18

申请号：US17320052

申请日：2021-05-13

Applicant: Becton, Dickinson and Company

Inventor： Devon Jensen ,  Katherine Lazaruk ,  Dennis Prosen ,  Ricelle A. Acob ,  Kai Liu

IPC: C12N15/10 ,  C12Q1/6858 ,  C07K14/705 ,  C07K14/725

Abstract: Disclosed herein include systems, methods, compositions, and kits for immune repertoire profiling. There are provided, in some embodiments, primer panels enabling the determination of the nucleotide sequence of the complete variable region of nucleic acids encoding mouse B cell receptor (BCR) and T cell receptor (TCR) polypeptides. In some embodiments, the method comprises single cell transcriptomic analysis.

公开(公告)号：US20210238661A1

公开(公告)日：2021-08-05

申请号：US17163177

申请日：2021-01-29

Applicant: Becton, Dickinson and Company

Inventor： Dennis Prosen ,  Katherine Lazaruk

IPC: C12Q1/6813

Abstract: Disclosed herein include systems, methods, compositions, and kits for 5′-based gene expression profiling and for whole transcriptome analysis (WTA) with random priming and extension (RPE). Blocker oligonucleotides capable of specifically binding to a portion of an oligonucleotide barcode are provided in some embodiments. The blocker oligonucleotides can reduce of the generation of undesirable extension products, such as, for example, the extension products of random primers hybridized to a portion of the oligonucleotide barcode. Immune repertoire profiling methods are also provided in some embodiments.

公开(公告)号：US20230348975A1

公开(公告)日：2023-11-02

申请号：US18187488

申请日：2023-03-21

Applicant: Becton, Dickinson and Company

Inventor： Eleen Shum ,  Katherine Lazaruk ,  Dennis E. Prosen ,  Devon Jensen ,  David Rosenfeld ,  Janice H. Lai ,  James Ghadiali ,  Christina Chang

IPC: C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6853

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q2525/173 ,  C12Q2525/191 ,  C12Q2525/204 ,  C12Q2531/113 ,  C12Q2563/185

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).

公开(公告)号：US20230272454A1

公开(公告)日：2023-08-31

申请号：US18190465

申请日：2023-03-27

Applicant: Becton, Dickinson and Company

Inventor： Devon Jensen ,  Katherine Lazaruk ,  Dennis Prosen ,  Ricelle A. Acob ,  Kai Liu

IPC: C12Q1/6806 ,  C07K14/705 ,  C07K14/725 ,  C12Q1/6858

CPC classification number: C12Q1/6806 ,  C07K14/70503 ,  C07K14/7051 ,  C12Q1/6858

Abstract: Disclosed herein include systems, methods, compositions, and kits for immune repertoire profiling. There are provided, in some embodiments, primer panels enabling the determination of the nucleotide sequence of the complete variable region of nucleic acids encoding mouse B cell receptor (BCR) and T cell receptor (TCR) polypeptides. In some embodiments, the method comprises single cell transcriptomic analysis.

公开(公告)号：US20210371909A1

公开(公告)日：2021-12-02

申请号：US17336055

申请日：2021-06-01

Applicant: Becton, Dickinson and Company

Inventor： Katherine Lazaruk

IPC: C12Q1/6818 ,  C12Q1/6809

Abstract: Disclosed herein include systems, methods, compositions, and kits for 5′-based gene expression profiling. Some embodiments provide synthetic particles (e.g., beads) associated with a first plurality of oligonucleotide barcodes and a second plurality of oligonucleotide barcodes. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3′ end with the first plurality of oligonucleotide barcodes and subsequently barcoded on the 5′ end following a template switching reaction and intermolecular hybridization with the first plurality of oligonucleotide barcodes and extension. Immune repertoire profiling methods are also provided in some embodiments.

公开(公告)号：US20250154560A1

公开(公告)日：2025-05-15

申请号：US18925473

申请日：2024-10-24

Applicant: Becton, Dickinson and Company

Inventor： Katherine Lazaruk

IPC: C12Q1/6818 ,  C12Q1/6809

Abstract: Disclosed herein include systems, methods, compositions, and kits for 5′-based gene expression profiling. Some embodiments provide synthetic particles (e.g., beads) associated with a first plurality of oligonucleotide barcodes and a second plurality of oligonucleotide barcodes. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3′ end with the first plurality of oligonucleotide barcodes and subsequently barcoded on the 5′ end following a template switching reaction and intermolecular hybridization with the first plurality of oligonucleotide barcodes and extension. Immune repertoire profiling methods are also provided in some embodiments.

公开(公告)号：US20240327906A1

公开(公告)日：2024-10-03

申请号：US18187449

申请日：2023-03-21

Applicant: Becton, Dickinson and Company

Inventor： Dennis Prosen ,  Katherine Lazaruk

IPC: C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q1/6804

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining expressions of proteins and genes simultaneously, and for sample indexing. The method can comprise extending a cellular component-binding reagent specific oligonucleotide hybridized to an oligonucleotide barcode to generate an extended cellular component-binding reagent specific oligonucleotide. The extended cellular component-binding reagent specific oligonucleotide can be separated from the oligonucleotide barcodes. The separated extended cellular component-binding reagent specific oligonucleotide can be amplified separately from barcoded cDNA.

公开(公告)号：US20240076801A1

公开(公告)日：2024-03-07

申请号：US18347010

申请日：2023-07-05

Applicant: Becton, Dickinson and Company

Inventor： Cynthia Sakofsky ,  Katherine Lazaruk ,  Margaret Nakamoto ,  Devon Joseph Jensen

IPC: C40B30/04 ,  C12N15/10 ,  C40B40/10 ,  C40B50/06

CPC classification number: C40B30/04 ,  C12N15/1065 ,  C12N15/1072 ,  C40B40/10 ,  C40B50/06

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining the expression of highly expressed proteins and lowly expressed proteins. In some embodiments, primers allowing generation of separate libraries for abundant AbSeq protein profiling oligonucleotides and scarce AbSeq protein profiling oligonucleotides are provided.

公开(公告)号：US20210214784A1

公开(公告)日：2021-07-15

申请号：US17147272

申请日：2021-01-12

Applicant: Becton, Dickinson and Company

Inventor： Dennis Prosen ,  Katherine Lazaruk

IPC: C12Q1/6874

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining expressions of proteins and genes simultaneously, and for sample indexing. The method can comprise extending a cellular component-binding reagent specific oligonucleotide hybridized to an oligonucleotide barcode to generate an extended cellular component-binding reagent specific oligonucleotide. The extended cellular component-binding reagent specific oligonucleotide can be separated from the oligonucleotide barcodes. The separated extended cellular component-binding reagent specific oligonucleotide can be amplified separately from barcoded cDNA.