公开(公告)号：US20230065324A1

公开(公告)日：2023-03-02

申请号：US17814042

申请日：2022-07-21

Applicant: Becton, Dickinson And Company

Inventor： Jue Fan ,  Jennifer Tsai ,  Eleen Shum ,  Lisha Deng ,  Glenn K. Fu

IPC: G06K19/06 ,  G16B40/00 ,  G16B30/00 ,  G16B40/10

Abstract: Disclosed herein are methods and systems for determining the numbers of targets. In some embodiments, the method comprise: stochastically barcoding targets using stochastic barcodes; obtaining sequencing data; for one or more of the targets: counting the number of molecular labels with distinct sequences associated with the target in the sequencing data; identifying clusters of molecular labels of the target using directional adjacency; collapsing the sequencing data using the clusters of molecular labels of the target identified; and estimating the number of the target.

公开(公告)号：US11365409B2

公开(公告)日：2022-06-21

申请号：US16400886

申请日：2019-05-01

Applicant: Becton, Dickinson and Company

Inventor： Eleen Shum ,  Christina Fan

IPC: C12N15/10 ,  C12Q1/6816

Abstract: Disclosed herein include systems, methods, compositions, and kits for molecular barcoding on the 5′-end of a nucleic acid target. After barcoding a nucleic acid target using an oligonucleotide barcode comprising a target binding region and a molecular label to generate a barcoded nucleic acid molecule, an oligonucleotide comprising a complement of the target binding region can be added to generate a barcoded nucleic acid molecule comprising the target-binding region and the complement of the target-binding region. A stem loop is formed with intra-molecular hybridization of the barcoded nucleic acid molecule, which can be extended to generate an extended barcoded nucleic acid molecule comprising the molecular label and a complement of the molecular label.

公开(公告)号：US20200248263A1

公开(公告)日：2020-08-06

申请号：US16748691

申请日：2020-01-21

Applicant: Becton, Dickinson and Company

Inventor： Margaret Nakamoto ,  Eleen Shum ,  Nidhanjali BANSAL ,  Christina Chang

IPC: C12Q1/6883 ,  G01N33/53

Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).

公开(公告)号：US12084712B2

公开(公告)日：2024-09-10

申请号：US16848241

申请日：2020-04-14

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Christina Chang ,  Christina Fan ,  Eleen Shum ,  Jody Martin ,  Nidhanjali Bansal ,  James Ghadiali ,  Katherine Lazaruk ,  Gretchen Yinbon Lam

IPC: C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12Q1/6869 ,  C12Q1/6876 ,  G01N33/532 ,  B82Y5/00 ,  C12Q1/6834 ,  C12Q1/6837

CPC classification number: C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12Q1/6869 ,  C12Q1/6876 ,  G01N33/532 ,  B82Y5/00 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2563/149 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2600/16 ,  G01N2458/10 ,  C12Q1/6869 ,  C12Q2525/179 ,  C12Q2563/131 ,  C12Q2563/179 ,  C12Q1/6869 ,  C12Q2563/131 ,  C12Q2563/179

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.

公开(公告)号：US11946095B2

公开(公告)日：2024-04-02

申请号：US16955657

申请日：2018-12-18

Applicant: Becton, Dickinson and Company

Inventor： Gretchen Yinbon Lam ,  Eleen Shum

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: Disclosed herein are methods, compositions, and kits for barcoding nucleic acids. For example, particles for barcoding are provided, where the particle comprises: a first plurality of oligonucleotide barcodes each comprising a target-binding region, and a second plurality of oligonucleotides each comprising a target-specific target-binding region.

公开(公告)号：US11834715B2

公开(公告)日：2023-12-05

申请号：US16748691

申请日：2020-01-21

Applicant: Becton, Dickinson and Company

Inventor： Margaret Nakamoto ,  Eleen Shum ,  Nidhanjali Bansal ,  Christina Chang

IPC: C12Q1/6883 ,  G01N33/53

CPC classification number: C12Q1/6883 ,  G01N33/5308 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/158

Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).

公开(公告)号：US20220315988A1

公开(公告)日：2022-10-06

申请号：US17830897

申请日：2022-06-02

Applicant: Becton, Dickinson and Company

Inventor： Katherine Lazaruk ,  Eleen Shum ,  Christina Chang

IPC: C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/6876

Abstract: Disclosed herein include systems, methods, compositions, and kits for PCR normalization. In some embodiments, after barcoding copies of a higher abundance target (e.g., a cDNA species), the barcoded copies are amplified using a pair of forward primers comprising one or more mismatches and a reverse primer. The amplified copies can be further linearly amplified using a forward primer comprising the sequence of one of the pair of forward primers, and a reverse primer.

公开(公告)号：US20220220549A1

公开(公告)日：2022-07-14

申请号：US17707409

申请日：2022-03-29

Applicant: Becton, Dickinson and Company

Inventor： Eleen Shum ,  Glenn K. Fu

IPC: C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6832 ,  C12Q1/6806 ,  C12N15/10

Abstract: Disclosed herein include methods and compositions for selectively amplifying and/or extending nucleic acid target molecules in a sample. The methods and compositions can, for example, reduce the amplification and/or extension of undesirable nucleic acid species in the sample, and/or allow selective removal of undesirable nucleic acid species in the sample.

公开(公告)号：US20210054436A1

公开(公告)日：2021-02-25

申请号：US16955657

申请日：2018-12-18

Applicant: Becton, Dickinson and Company

Inventor： Gretchen Yinbon Lam ,  Eleen Shum

IPC: C12Q1/6806

Abstract: Disclosed herein are methods, compositions, and kits for barcoding nucleic acids. For example, particles for barcoding are provided, where the particle comprises: a first plurality of oligonucleotide barcodes each comprising a target-binding region, and a second plurality of oligonucleotides each comprising a target-specific target-binding region.

公开(公告)号：US20180088112A1

公开(公告)日：2018-03-29

申请号：US15715028

申请日：2017-09-25

Applicant: Cellular Research, Inc. ,  Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  C12Q1/68 ,  G01N33/543 ,  C12N15/10

CPC classification number: G01N33/566 ,  B01J2219/00646 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07K2319/01 ,  C12N15/1006 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  G01N33/505 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/6842 ,  G01N33/6857 ,  G06F19/00 ,  G16B25/10 ,  G16B99/00 ,  G16H10/20 ,  G16H20/13 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.