发明申请
US20110294691A1 ENHANCED ON-CHIP SERS BASED BIOMOLECULAR DETECTION USING ELECTROKINETICALLY ACTIVE MICROWELLS
有权
使用电动活性微球增强基于片上SERS的生物分子检测
- 专利标题： ENHANCED ON-CHIP SERS BASED BIOMOLECULAR DETECTION USING ELECTROKINETICALLY ACTIVE MICROWELLS
- 专利标题（中）： 使用电动活性微球增强基于片上SERS的生物分子检测
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申请号： US13124296申请日： 2009-10-14
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公开(公告)号： US20110294691A1公开(公告)日： 2011-12-01
- 发明人: David Erickson , Yun Suk Huh , Carl A. Batt , Adam Joseph Lowe
- 申请人： David Erickson , Yun Suk Huh , Carl A. Batt , Adam Joseph Lowe
- 专利权人： Cornell University
- 当前专利权人： Cornell University
- 国际申请： PCT/US09/60675 WO 20091014
- 主分类号： C40B30/04
- IPC分类号： C40B30/04 ; C12M1/34 ; C07H1/00 ; C12Q1/68 ; C07H21/00
摘要：
A method for detecting target nucleic acids such as SNPs is provided. The method comprises performing a ligase detection reaction (LDR), performing surface enhanced Raman scattering (SERS) on the LDR, and analyzing the outcome of the LDR using analysis and/or quantification of the SERS by detecting an emitted Raman signature. The LDR-SERS method can be used for sensitive and specific detection of any nucleic acid sequence of interest. A microfluidic SERS detection device is also provided. The device comprises electrokinetically active microwells for mixing and concentrating analytes and in which analytes can be quantified. The device can be used for performing the LDR-SERS method in optofluidic chip format.
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