发明授权
- 专利标题： Process for the production of permanently culturable animal and human cell lines and the use thereof
- 专利标题（中）： 生产永久文化动物和人体细胞系的方法及其用途
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申请号： US590602申请日： 1990-09-25
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公开(公告)号： US5114847A公开(公告)日： 1992-05-19
- 发明人: Herbert Jungfer , Heinrich Barchet , Winfried Albert
- 申请人： Herbert Jungfer , Heinrich Barchet , Winfried Albert
- 申请人地址： DEX Mannheim
- 专利权人： Boehringer Mannheim GmbH
- 当前专利权人： Boehringer Mannheim GmbH
- 当前专利权人地址： DEX Mannheim
- 优先权： DEX3216650 19820504; DEX3245665 19821209
- 主分类号： G01N33/577
- IPC分类号： G01N33/577 ; A61K35/12 ; A61K38/16 ; A61K39/395 ; C07K14/00 ; C07K14/52 ; C07K16/00 ; C12N15/00 ; C12N15/02 ; C12P21/08 ; C12R1/91 ; G01N33/48
摘要：
A method for obtaining permanently culturable human and animal cell lines, as well as uses for these cell lines, are disclosed. The method involves fusing cells of a non-immortal cell line with cytoplasts or cytoplasma fractions of "immortal", transformed cells such as immortal myeloma cells, ascites-tumor cells or Epstein Barr Virus infected cells. Once fusion takes place the product is a permanently culturable variant of the previously normal cell line.
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