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11 条记录 (耗时 0.013 秒)

    Digitalis antibodies, process for the preparation thereof and the use thereof for the therapy of digitalis intoxications
    2.
    发明授权
    Digitalis antibodies, process for the preparation thereof and the use thereof for the therapy of digitalis intoxications 失效
    洋地黄抗体，其制备方法及其用于治疗洋地黄中毒的用途

    公开(公告)号：US4742159A

    公开(公告)日：1988-05-03

    申请号：US563217

    申请日：1983-12-19

    申请人： Hans-Georg Batz ,  Herbert Jungfer ,  Helmut Lenz ,  Albert Roder

    发明人： Hans-Georg Batz ,  Herbert Jungfer ,  Helmut Lenz ,  Albert Roder

    IPC分类号： C07K14/415 ,  A61K38/00 ,  A61K39/395 ,  A61K39/44 ,  A61P39/02 ,  C07K1/22 ,  C07K14/00 ,  C07K14/435 ,  C07K16/00 ,  C07K16/16 ,  G01N33/53 ,  G01N33/94 ,  C07K13/00 ,  C12P21/00 ,  C12Q1/00

    CPC分类号： C07K16/16 ,  G01N33/9453 ,  A61K38/00 ,  Y10S424/809 ,  Y10S530/866

    摘要： The present invention provides a process for the preparation of digitalis antibodies in which appropriate mammals are immunized with a digoxin bound to protein, the animal serum is obtained, the immune globulin-containing protein fraction is separated in known manner, the immunologically-active globulins are adsorbed on an immunologically-active column and separated from the other proteins, the antibodies are again eluted from the column and the Fab fractions are split with papain and purified, wherein, as immunologically-active adsorbent, there is used an inorganic matrix of large surface area to which digitoxin aldehyde is bound via a spacer which cannot be split with papain and the splitting off of the Fab fraction from the antibodies is carried out on the matrix.The present invention also provides digitalis antibodies obtained by this process, which antibodies can be used for the therapy of digitalis intoxications and for preparing immunological reagents for the determination of digitalis glycosides.

    摘要翻译： 本发明提供了一种制备洋地黄抗体的方法，其中用与蛋白质结合的地高辛免疫合适的哺乳动物，获得动物血清，以已知方式分离含免疫球蛋白的蛋白质级分，免疫活性球蛋白 吸附在免疫活性柱上并与其他蛋白质分离，抗体再次从柱中洗脱出来，Fab级分用木瓜蛋白酶分离纯化，其中作为免疫活性吸附剂，使用大面积的无机基质 通过不能与木瓜蛋白酶分离的间隔基结合数毒素醛的区域，并且在基质上进行Fab级分与抗体的分离。 本发明还提供通过该方法获得的洋地黄抗体，其抗体可用于治疗洋地黄中毒，并用于制备用于测定洋地黄糖苷的免疫试剂。

    Monoclonal antibodies against melanoma
    3.
    发明授权
    Monoclonal antibodies against melanoma 失效
    针对黑色素瘤的单克隆抗体

    公开(公告)号：US5610280A

    公开(公告)日：1997-03-11

    申请号：US214020

    申请日：1994-03-15

    申请人： Michael Brandt ,  Josef Endl ,  Herbert Jungfer ,  Winfried Albert

    发明人： Michael Brandt ,  Josef Endl ,  Herbert Jungfer ,  Winfried Albert

    IPC分类号： A61K39/395 ,  A61P35/00 ,  C07K16/00 ,  C07K16/30 ,  C07K19/00 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/574 ,  G01N33/577 ,  A61K51/10 ,  C12N5/12 ,  G01N33/53

    CPC分类号： C07K16/3053

    摘要： The present invention provides a human monoclonal antibody against melanoma, characterized in that it binds to the gangliosides GM3 and GD3 but essentially does not bind to the gangliosides GM1, GM2, GD1a, GD1b and GD2, the binding of the antibody to the gangliosides having been determined by immune staining after thin layer chromatographic separation of the gangliosides. The present invention also provides a process for the production of human monoclonal antibodies directed against melanoma, wherein, without previous immunization, B-lymphocytes are isolated from a healthy person, the isolated B-lymphocytes are immortalized, antibodies from the immortalized B-lymphocytes are screened by immune-histochemical analysis for binding against melanoma and/or melanoma metastases, the positively reacting B-lymphocytes are selected, cultured and monoclonal antibodies obtained therefrom.

    摘要翻译： 本发明提供了针对黑素瘤的人单克隆抗体，其特征在于其结合神经节苷脂GM3和GD3，但基本上不结合神经节苷脂GM1，GM2，GD1a，GD1b和GD2，抗体与神经节苷脂的结合 通过神经节苷脂薄层色谱分离后的免疫染色测定。 本发明还提供了一种制备针对黑素瘤的人单克隆抗体的方法，其中，在没有先前免疫的情况下，从健康人分离B淋巴细胞，分离的B淋巴细胞被永生化，来自永生化B淋巴细胞的抗体是 通过用于结合黑素瘤和/或黑素瘤转移的免疫组织化学分析进行筛选，选择阳性反应性B淋巴细胞，培养和从其获得的单克隆抗体。

    Tumoricidal T lymphocytes
    4.
    发明授权
    Tumoricidal T lymphocytes 失效
    杀肿瘤T淋巴细胞

    公开(公告)号：US06576466B2

    公开(公告)日：2003-06-10

    申请号：US08513865

    申请日：1995-12-04

    申请人： Herbert Jungfer ,  Heinrich Barchet ,  Winfred Albert ,  Ulrich Weidle

    发明人： Herbert Jungfer ,  Heinrich Barchet ,  Winfred Albert ,  Ulrich Weidle

    IPC分类号： C12N506

    CPC分类号： A61K39/0011 ,  A61K2039/5158

    摘要： The present invention concerns a mammalian cell line which when co-cultured with lymphocytes during which allogenic stimulation is avoided activates lymphocytes fo form tumoricidal cells, a process for the production of tumoricidal T lymphocytes by co-culturing lymphocytes with this cell line, the tumoricidal T lymphocytes obtained by means of this process and the use of the cells according to the present invention for the production of a therapeutic agent which can be used in tumour therapy.

    摘要翻译： 本发明涉及哺乳动物细胞系，当与避免同种异体刺激的淋巴细胞共同培养时，可激活形成杀肿瘤细胞的淋巴细胞，通过与该细胞系共培养淋巴细胞产生杀肿瘤T淋巴细胞的方法，杀肿瘤T 通过该方法获得的淋巴细胞和根据本发明的细胞用于生产可用于肿瘤治疗的治疗剂。

    Monoclonal antibodies of isotype IgG which bind specifically to glutamate decarboxylase, and uses thereof
    7.
    发明授权
    Monoclonal antibodies of isotype IgG which bind specifically to glutamate decarboxylase, and uses thereof 失效
    与谷氨酸脱羧酶特异性结合的同种型IgG的单克隆抗体及其用途

    公开(公告)号：US06214568B1

    公开(公告)日：2001-04-10

    申请号：US09124410

    申请日：1998-07-29

    申请人： Josef Endl ,  Michael Brandt ,  Herbert Jungfer ,  Winfried Albert ,  Rosemarie Kientsch-Engel ,  Werner Scherbaum ,  Wiltrud Richter ,  Thomas Eiermann

    发明人： Josef Endl ,  Michael Brandt ,  Herbert Jungfer ,  Winfried Albert ,  Rosemarie Kientsch-Engel ,  Werner Scherbaum ,  Wiltrud Richter ,  Thomas Eiermann

    IPC分类号： G01N33573

    CPC分类号： C07K16/40 ,  C07K14/47 ,  C07K16/18 ,  C07K16/4241 ,  C07K2317/21

    摘要： The invention concerns human monoclonal antibodies of the IgG isotype against human pancreatic islet cells which can be obtained by immortalizing human lymphocytes of prediabetics or diabetics, treating the culture supernatant of the immortalized cells with a conjugate of antibodies against human Fc &ggr; and a label, subsequently treating with human immunoglobulin, incubating with immobilized human pancreatic islet cells identifying an immortalized human cell culture which produces an antibody against pancreatic islet cells via determination of the label bound to the immobilized islet cells, isolating a human immortalized cell which produces this antibody, propagating this immortalized cell and isolating the monoclonal antibody produced by these cells. The invention also concerns a process for the isolation of an islet cell antigen to which such antibodies bind as well as a method for the determination of antibodies against an islet cell antigen of the pancreas.

    摘要翻译： 本发明涉及针对人胰岛细胞的IgG同种型的人单克隆抗体，其可以通过使老年糖尿病患者或糖尿病患者的人淋巴细胞永生化而获得，用抗Fcγ和标记的抗体缀合物处理永生化细胞的培养物上清液，随后 用人免疫球蛋白处理，与固定的人胰岛细胞孵育，鉴定永生化​​的人细胞培养物，其通过测定与固定化胰岛细胞结合的标记产生抗胰岛细胞的抗体，分离产生该抗体的人永生化细胞，传播该抗体 永生化细胞并分离由这些细胞产生的单克隆抗体。 本发明还涉及用于分离这种抗体结合的胰岛细胞抗原的方法以及用于测定抗胰岛细胞抗原的抗体的方法。

    Method for mammalian cell culture
    8.
    发明授权
    Method for mammalian cell culture 失效
    哺乳动物细胞培养方法

    公开(公告)号：US5286646A

    公开(公告)日：1994-02-15

    申请号：US763867

    申请日：1991-09-20

    申请人： Michael J. Kearns ,  Michael J. Comer ,  Ulrich Steegmans ,  Herbert Jungfer

    发明人： Michael J. Kearns ,  Michael J. Comer ,  Ulrich Steegmans ,  Herbert Jungfer

    IPC分类号： C12M3/06 ,  C12N5/06

    CPC分类号： C12M29/16 ,  C12M27/02

    摘要： The invention presents a method for the culturing of mammalian cells. This method involves the use of a bioreactor, which contains a sample of mammalian cells in a culture medium containing large molecules. Positioned inside the bioreactor is a semipermeable membrane which defines a space separated from the bioreactor by the semipermeable membrane. A nutrient medium flows through this separated space and, via virtue of the semipermeable nature of the separating membrane, nutrient pass therethrough into the culture medium, while cellular waste products pass into the separated space. The semipermeable membrane is selected so that the cells and large molecules, such as proteinaceous materials, cannot pass through the membrane, but remain in the bioreactor.

    摘要翻译： 本发明提出了哺乳动物细胞的培养方法。 该方法涉及使用含有大分子的培养基中的哺乳动物细胞样品的生物反应器。 定位在生物反应器内的是半透膜，其限定了通过半透膜与生物反应器分离的空间。 营养培养基流过该分离的空间，并且由于分离膜的半透性，营养物通过其进入培养基，而细胞废物进入分离的空间。 选择半透膜，使得细胞和大分子（例如蛋白质材料）不能通过膜，而是保留在生物反应器中。