发明授权
US06943279B1 Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
失效
氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法
- 专利标题： Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
- 专利标题（中）： 氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法
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申请号： US09658835申请日： 2000-09-08
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公开(公告)号： US06943279B1公开(公告)日： 2005-09-13
- 发明人: Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Aragula Gururaj Rao , Oswald R. Crasta , Otto Folkerts
- 申请人： Jonathan P. Duvick , Jacob T. Gilliam , Joyce R. Maddox , Aragula Gururaj Rao , Oswald R. Crasta , Otto Folkerts
- 申请人地址： US IA Johnston
- 专利权人： Pioneer Hi-Bred International, Inc.
- 当前专利权人： Pioneer Hi-Bred International, Inc.
- 当前专利权人地址： US IA Johnston
- 代理机构： McKee, Voorhees & Sease, P.L.C.
- 主分类号： C12N9/06
- IPC分类号： C12N9/06 ; C12N15/09 ; C12N15/31 ; C12N15/82 ; A10H1/00 ; A10H5/10
摘要：
The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella airovirens. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.