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    Method for the integration of foreign DNA into eukaryotic genomes
    2.
    发明授权
    Method for the integration of foreign DNA into eukaryotic genomes 有权
    将外源DNA整合到真核生物基因组中的方法

    公开(公告)号：US07223601B2

    公开(公告)日：2007-05-29

    申请号：US10353445

    申请日：2003-01-29

    申请人： Christopher L. Baszczynski ,  Leszek Alexander Lyznik ,  William J. Gordon-Kamm ,  Xueni Guan ,  Aragula Gururaj Rao ,  Laura A. Tagliani

    发明人： Christopher L. Baszczynski ,  Leszek Alexander Lyznik ,  William J. Gordon-Kamm ,  Xueni Guan ,  Aragula Gururaj Rao ,  Laura A. Tagliani

    IPC分类号： C12N15/87

    CPC分类号： C12N15/90 ,  C07K2319/00 ,  C12N9/00 ,  C12N15/8203 ,  C12N15/8205 ,  C12N15/8213

    摘要： Compositions and methods for introducing a DNA of interest into a genomic target site are provided. In particular, the methods and compositions involve the use of a combination of target sites for two site specific recombinases and expression of a chimeric recombinase with dual target site specificity. Thus, the compositions comprise novel site-specific recombinases with specificities to multiple target sites, and nucleotide sequences and expression cassettes encoding these recombinases or target sites. The methods involve transforming a eukaryotic cell having target sites for the novel recombinase with a DNA of interest that is flanked by corresponding target sites. Expression of the recombinase results in integration of the DNA of interest into the genome of the cell. The compositions and methods of the invention have use in the construction of stably transformed eukaryotic cells, and in particular, plant cells. The methods result in the efficient targeted genomic integration of DNA by site-specific recombination.

    摘要翻译： 提供了将目的DNA引入基因组靶位点的组合物和方法。 特别地，所述方法和组合物涉及使用两个位点特异性重组酶的靶位点的组合和具有双重靶位点特异性的嵌合重组酶的表达。 因此，组合物包含对多个靶位点具有特异性的新的位点特异性重组酶，以及编码这些重组酶或靶位点的核苷酸序列和表达盒。 所述方法包括用具有相应靶位点侧翼的感兴趣的DNA转化具有新重组酶靶位点的真核细胞。 重组酶的表达导致感兴趣的DNA整合到细胞的基因组中。 本发明的组合物和方法用于构建稳定转化的真核细胞，特别是植物细胞。 该方法通过位点特异性重组导致DNA的有效靶向基因组整合。

    PEPTIDE DOMAINS THAT BIND SMALL MOLECULES OF INDUSTRIAL SIGNIFICANCE
    5.
    发明申请
    PEPTIDE DOMAINS THAT BIND SMALL MOLECULES OF INDUSTRIAL SIGNIFICANCE 有权
    包含工业意义的小分子的肽区域

    公开(公告)号：US20130116138A1

    公开(公告)日：2013-05-09

    申请号：US13575834

    申请日：2011-01-28

    申请人： Thomas P. Binder ,  Aragula Gururaj Rao ,  Yasufumi Yamamoto ,  Paul Hanke

    发明人： Thomas P. Binder ,  Aragula Gururaj Rao ,  Yasufumi Yamamoto ,  Paul Hanke

    IPC分类号： C07K7/06 ,  C40B30/06

    CPC分类号： C07K7/06 ,  C07K5/08 ,  C07K7/08 ,  C07K14/00

    摘要： Described herein are small peptide domains and consensus sequences that bind small target molecules of industrial importance, e.g., metals such as nickel, β carotene, and isoflavones such as genistein. Also described are fusion proteins containing such binding domains fused to proteins or to peptide domains like GST or GBD that bind other ligands and can be used to immobilize the target binding domain on a support. One class of fusion proteins that is useful in industrial settings are fusions that contain concatemers of target binding domains, which increases the binding equivalents per molecule.

    摘要翻译： 本文描述的是结合工业重要性的小靶分子的小肽结构域和共有序列，例如金属如镍，β-胡萝卜素和异黄酮如染料木素。 还描述了含有融合蛋白质或结合其他配体的肽结构域（如GST或GBD）的融合蛋白，并可用于将靶结合结构域固定在载体上。 在工业环境中有用的一类融合蛋白是融合靶结合域的融合物，这增加了每个分子的结合当量。

    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
    6.
    发明授权
    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 有权
    氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法

    公开(公告)号：US07241934B2

    公开(公告)日：2007-07-10

    申请号：US10743891

    申请日：2003-12-22

    申请人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Aragula Gururaj Rao ,  Oswald R. Crasta ,  Otto Folkerts

    发明人： Jonathan P. Duvick ,  Jacob T. Gilliam ,  Joyce R. Maddox ,  Aragula Gururaj Rao ,  Oswald R. Crasta ,  Otto Folkerts

    IPC分类号： C12N15/82 ,  C12N15/31 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N9/0022 ,  C12N15/8242 ,  C12N15/8282

    摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella atrovirens. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.

    摘要翻译： 本发明提供了从Exophiala spinifera和Rhinocladiella atvirens分离的酶APAO的多核苷酸和相关多肽。 可以突变多核苷酸以除去糖基化位点和半胱氨酸残基。 另外，本发明提供了重组表达盒，宿主细胞，转基因植物和转基因种子。 本发明还提供了含有APAO和伏马菌素酯酶的多核苷酸。 此外，本发明提供了在原核和真核系统中生产APAO酶的方法，用于检测伏马毒素的方法和用于鉴定转化的植物细胞的方法。 还公开了降解植物，谷物，谷物加工，青贮饲料，粮食作物和动物饲料中真菌毒素的方法。

    Peptide domains that bind small molecules of industrial significance
    8.
    发明授权
    Peptide domains that bind small molecules of industrial significance 有权
    结合小分子工业意义的肽结构域

    公开(公告)号：US09447150B2

    公开(公告)日：2016-09-20

    申请号：US13575834

    申请日：2011-01-28

    申请人： Thomas P. Binder ,  Aragula Gururaj Rao ,  Yasufumi Yamamoto ,  Paul D. Hanke

    发明人： Thomas P. Binder ,  Aragula Gururaj Rao ,  Yasufumi Yamamoto ,  Paul D. Hanke

    IPC分类号： C07K7/06 ,  C40B30/06 ,  C07K14/00 ,  C07K7/08

    CPC分类号： C07K7/06 ,  C07K5/08 ,  C07K7/08 ,  C07K14/00

    摘要： Described herein are small peptide domains and consensus sequences that bind small target molecules of industrial importance, e.g., metals such as nickel, β carotene, and isoflavones such as genistein. Also described are fusion proteins containing such binding domains fused to proteins or to peptide domains like GST or CBD that bind other ligands and can be used to immobilize the target binding domain on a support. One class of fusion proteins that is useful in industrial settings are fusions that contain concatemers of target binding domains, which increases the binding equivalents per molecule.

    摘要翻译： 本文描述的是结合工业重要的小靶分子的小肽结构域和共有序列，例如金属如镍，β-胡萝卜素和异黄酮如染料木素。 还描述了融合蛋白，其含有融合蛋白质或结合其它配体的肽结构域（如GST或CBD）的融合蛋白，并可用于将靶结合结构域固定在载体上。 在工业环境中有用的一类融合蛋白是融合靶结合域的融合物，这增加了每个分子的结合当量。