- 专利标题: Aldolases, nucleic acids encoding them and methods for making and using them
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申请号: US12281052申请日: 2007-03-07
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公开(公告)号: US08546118B2公开(公告)日: 2013-10-01
- 发明人: David P. Weiner , Lishan Zhao , Peter Luginbuhl , Ellen Burke , Toby Richardson , Paula M. Hicks
- 申请人: David P. Weiner , Lishan Zhao , Peter Luginbuhl , Ellen Burke , Toby Richardson , Paula M. Hicks
- 申请人地址: US CA San Diego
- 专利权人: Verenium Corporation
- 当前专利权人: Verenium Corporation
- 当前专利权人地址: US CA San Diego
- 代理机构: Verenium Corporation
- 代理商 Brian W. Siddons
- 国际申请: PCT/US2007/063515 WO 20070307
- 国际公布: WO2007/103989 WO 20070913
- 主分类号: C12N9/10
- IPC分类号: C12N9/10 ; C12N15/00 ; C12N1/20 ; C12Q1/68 ; C12P21/04 ; C12P21/06 ; C07H21/04
摘要:
This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.