公开(公告)号：US08865241B1

公开(公告)日：2014-10-21

申请号：US12893322

申请日：2010-09-29

申请人： Stan Bower ,  Ellen Burke ,  Nancy E. Harding ,  Yamini N. Patel ,  J. Carrie Schneider ,  Dagmar Meissner ,  Neil A. Morrison ,  Ralph Bezanson

发明人： Stan Bower ,  Ellen Burke ,  Nancy E. Harding ,  Yamini N. Patel ,  J. Carrie Schneider ,  Dagmar Meissner ,  Neil A. Morrison ,  Ralph Bezanson

IPC分类号： A23L1/05 ,  C07H17/00 ,  C08B37/00 ,  C07H1/06 ,  C07H1/08

CPC分类号： C08B37/006 ,  A23L21/10 ,  A23L21/15 ,  A23L29/269 ,  C12N1/20 ,  C12P7/62 ,  C12P19/04

摘要： The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient Sphingans. The invention is also directed to a process for preparing a clarified Sphingan solution comprising heating aqueous Sphingan solution, in particular PHB-deficient Sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified Sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.

摘要翻译： 本发明涉及鞘氨醇单胞菌属的突变菌株，其在编码参与多羟基丁酸酯（“PHB”）合成的蛋白质的至少一个基因中具有突变，其允许突变菌株产生PHB缺乏的鞘氨醇。 本发明还涉及一种制备澄清的鞘氨醇溶液的方法，其包括将鞘氨醇水溶液，特别是PHB缺乏的鞘氨醇溶液加热至约30℃至约70℃的澄清温度，并用 澄清剂和酶。 此外，本发明涉及包含PHB缺乏和/或澄清的鞘氨醇的食品或工业产品。 本发明的一个具体实施方案涉及澄清的PHB缺陷型高酰基结冷胶及其制备方法。

公开(公告)号：US08198064B2

公开(公告)日：2012-06-12

申请号：US11292366

申请日：2005-12-02

申请人： Stan Bower ,  Ellen Burke ,  Nancy E. Harding ,  Yamini N. Patel ,  J. Carrie Schneider ,  Dagmar Meissner ,  Neil A. Morrison ,  Ralph Bezanson

发明人： Stan Bower ,  Ellen Burke ,  Nancy E. Harding ,  Yamini N. Patel ,  J. Carrie Schneider ,  Dagmar Meissner ,  Neil A. Morrison ,  Ralph Bezanson

IPC分类号： C12N1/12 ,  C12N15/00 ,  C12N9/10 ,  C07H21/02 ,  C07H21/04

CPC分类号： C08B37/006 ,  A23L21/10 ,  A23L21/15 ,  A23L29/269 ,  C12N1/20 ,  C12P7/62 ,  C12P19/04

摘要： The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.

摘要翻译： 本发明涉及鞘氨醇单胞菌属突变菌株，其在编码参与多羟基丁酸酯（“PHB”）合成的蛋白质的至少一个基因中具有突变，其允许突变株产生PHB缺陷的鞘氨醇。 本发明还涉及一种制备澄清的鞘氨醇溶液的方法，其包括加热水性鞘氨醇溶液，特别是PHB缺陷的鞘氨醇溶液至约30℃至约70℃的澄清温度，并用 澄清剂和酶。 此外，本发明涉及包含PHB缺陷和/或澄清的鞘氨醇的食品或工业产品。 本发明的一个具体实施方案涉及澄清的PHB缺陷型高酰基结冷胶及其制备方法。

公开(公告)号：US20110045547A1

公开(公告)日：2011-02-24

申请号：US12828714

申请日：2010-07-01

申请人： Mervyn L. de Souza ,  Wei Niu ,  Jose Laplaza ,  Christopher Solheid ,  Sherry R. Kollmann ,  Joshua M. Lundorff ,  Paula M. Hicks ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  David P. Weiner ,  Ellen Burke ,  Peter Luginbuhl ,  Analia Bueno ,  Joslin Cuenca

发明人： Mervyn L. de Souza ,  Wei Niu ,  Jose Laplaza ,  Christopher Solheid ,  Sherry R. Kollmann ,  Joshua M. Lundorff ,  Paula M. Hicks ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  David P. Weiner ,  Ellen Burke ,  Peter Luginbuhl ,  Analia Bueno ,  Joslin Cuenca

IPC分类号： C12P13/22 ,  C12P17/10

CPC分类号： C12N9/90 ,  C12P13/06 ,  C12P13/22 ,  C12P17/10

摘要： The invention provides for isomerase (e.g., racemase) and epimerase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such isomerase (e.g., racemase) and epimerase nucleic acids and polypeptides.

摘要翻译： 本发明提供异构酶（例如消旋酶）和差向异构酶多肽以及编码这种多肽的核酸。 还提供了使用这种异构酶（例如消旋酶）和差向异构酶核酸和多肽的方法。

公开(公告)号：US20100189706A1

公开(公告)日：2010-07-29

申请号：US12525303

申请日：2008-01-30

申请人： Cathy Chang ,  Justin T. Stege ,  Nahla Aboushadi ,  Gordana Djordjevic ,  Ellen Burke ,  Peter Luginbuhl ,  Mark Dycaico ,  Toby Richardson ,  John Poland ,  Ying Hefner ,  Stacy Marie Miles

发明人： Cathy Chang ,  Justin T. Stege ,  Nahla Aboushadi ,  Gordana Djordjevic ,  Ellen Burke ,  Peter Luginbuhl ,  Mark Dycaico ,  Toby Richardson ,  John Poland ,  Ying Hefner ,  Stacy Marie Miles

IPC分类号： A61K38/44 ,  A61K38/46 ,  C12N11/16 ,  A23L1/28 ,  C12P21/06 ,  C12P19/34 ,  C12P7/04 ,  C12P7/16 ,  C12P7/06 ,  C12N11/14 ,  C12N11/00 ,  C12N11/08 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/42 ,  C12N5/10 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12N15/63 ,  C12P1/00 ,  D21C3/00 ,  C40B40/06 ,  C40B40/10 ,  C11D7/42 ,  C07K16/00 ,  C07H21/04 ,  A01K67/00 ,  A01H5/00

CPC分类号： C12P19/14 ,  C12N9/2437 ,  C12N9/2445 ,  C12P7/04 ,  C12P19/02 ,  C12Y302/01021 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： The invention provides polypeptides having a lignocellulolytic activity, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a β-xylosidase), an arabinofuranosidase, and/or a glucose oxidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides that can enzymatically process (hydrolyze) sugarcane bagasse, i.e., for sugarcane bagasse degradation, or for biomass processing, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts; for example, the invention provides a multi-enzyme system that can hydrolyze polysaccharides in a bagasse component of sugarcane processed in sugar mills. The invention provides enzymes for the bioconversion of lignocellulosic residues into fermentable sugars; and these sugars can be used as a chemical feedstock for the production of ethanol and fuels, including biofuels such as bioethanol, biopropanol, biobutanol and biodiesels.

摘要翻译： 本发明提供具有木质纤维素分解活性的多肽，例如糖基水解酶，纤维素酶，内切葡聚糖酶，纤维二糖水解酶，β-葡糖苷酶，木聚糖酶，甘露聚糖酶，木糖苷酶（例如，α-氧化酶），阿拉伯呋喃糖苷酶， 和/或葡萄糖氧化酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明提供了可以酶促加工（水解）甘蔗渣，即甘蔗渣降解或用于生物量加工的多肽，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 在一个实施方案中，本发明提供本发明的多肽的耐热性和耐热性形式。 本发明的多肽可用于各种药物，农业和工业环境; 例如，本发明提供了一种可以在糖厂加工的甘蔗的蔗渣组分中水解多糖的多酶体系。 本发明提供用于将木质纤维素残基生物转化成可发酵糖的酶; 并且这些糖可以用作生产乙醇和燃料的化学原料，包括生物燃料如生物乙醇，生物丙醇，生物丁醇和生物柴油。

公开(公告)号：US20080020435A1

公开(公告)日：2008-01-24

申请号：US11714909

申请日：2007-03-07

申请人： Ellen Burke ,  Steven Gort ,  Paula Hicks ,  Peter Luginbuhl ,  Sara McFarlan ,  Toby Richardson ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

发明人： Ellen Burke ,  Steven Gort ,  Paula Hicks ,  Peter Luginbuhl ,  Sara McFarlan ,  Toby Richardson ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

IPC分类号： C12P17/10

CPC分类号： C12P17/10 ,  C12N9/88 ,  C12P13/001 ,  Y02P20/52

摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

摘要翻译： 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可以用于各种药物，农业和工业环境。 在一些实施方案中，本发明提供了可用于制备R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和莫纳甜的某些立体异构体的多肽和生物合成途径，例如R ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的某些立体异构体，例如R，R和S，R构型及其盐。

公开(公告)号：US20060252135A1

公开(公告)日：2006-11-09

申请号：US11411229

申请日：2006-04-26

申请人： Brian Brazeau ,  Ellen Burke ,  Mervyn DeSouza ,  Steven Gort ,  Paula Hicks ,  Sherry Kollmann ,  Peter Luginbuhl ,  Sara McFarlan ,  Toby Richardson ,  Fernando Sanchez-Riera ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

发明人： Brian Brazeau ,  Ellen Burke ,  Mervyn DeSouza ,  Steven Gort ,  Paula Hicks ,  Sherry Kollmann ,  Peter Luginbuhl ,  Sara McFarlan ,  Toby Richardson ,  Fernando Sanchez-Riera ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

IPC分类号： C12N9/10 ,  C12P13/22 ,  C12N1/21

CPC分类号： C12P17/10 ,  C12N9/1096 ,  C12N9/90

摘要： Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

公开(公告)号：US08546118B2

公开(公告)日：2013-10-01

申请号：US12281052

申请日：2007-03-07

申请人： David P. Weiner ,  Lishan Zhao ,  Peter Luginbuhl ,  Ellen Burke ,  Toby Richardson ,  Paula M. Hicks

发明人： David P. Weiner ,  Lishan Zhao ,  Peter Luginbuhl ,  Ellen Burke ,  Toby Richardson ,  Paula M. Hicks

IPC分类号： C12N9/10 ,  C12N15/00 ,  C12N1/20 ,  C12Q1/68 ,  C12P21/04 ,  C12P21/06 ,  C07H21/04

CPC分类号： C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

公开(公告)号：US08043837B2

公开(公告)日：2011-10-25

申请号：US11714909

申请日：2007-03-07

申请人： Ellen Burke ,  Steven John Gort ,  Paula M. Hicks ,  Peter Luginbuhl ,  Sara C. McFarlan ,  Toby Richardson ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

发明人： Ellen Burke ,  Steven John Gort ,  Paula M. Hicks ,  Peter Luginbuhl ,  Sara C. McFarlan ,  Toby Richardson ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

IPC分类号： C12P17/10 ,  C12P13/22 ,  C12P21/06 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12P17/10 ,  C12N9/88 ,  C12P13/001 ,  Y02P20/52

摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

摘要翻译： 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可以用于各种药物，农业和工业环境。 在一些实施方案中，本发明提供了可用于制备R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和莫纳甜的某些立体异构体的多肽和生物合成途径，例如R ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的某些立体异构体，例如R，R和S，R构型及其盐。

公开(公告)号：US20110027346A1

公开(公告)日：2011-02-03

申请号：US12303088

申请日：2007-05-29

申请人： David Weiner ,  Alexander Varvak ,  Toby Richardson ,  Mircea Podar ,  Ellen Burke ,  Shaun Healey

发明人： David Weiner ,  Alexander Varvak ,  Toby Richardson ,  Mircea Podar ,  Ellen Burke ,  Shaun Healey

IPC分类号： A61K9/127 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10 ,  C12N1/15 ,  C12N1/19 ,  A61P7/00 ,  C12N9/88 ,  C12N11/16 ,  C12N11/14 ,  C12N11/08 ,  C40B40/10 ,  C12P7/40 ,  A61K38/51 ,  A61K9/14 ,  A23C9/12

CPC分类号： C12N9/88

摘要： This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.

摘要翻译： 本发明提供了具有裂解酶活性的多肽，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明涉及具有氨裂解酶活性的多肽，例如苯丙氨酸氨裂解酶，酪氨酸氨裂解酶和/或组氨酸氨裂解酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸 和多肽。 本发明的多肽可用于多种药物，农业和工业环境。

公开(公告)号：US20100095390A1

公开(公告)日：2010-04-15

申请号：US12281052

申请日：2007-03-07

申请人： David P. Weiner ,  Lishan Zhao ,  Peter Luginbuhl ,  Ellen Burke ,  Toby H. Richardson ,  Paula M. Hicks

发明人： David P. Weiner ,  Lishan Zhao ,  Peter Luginbuhl ,  Ellen Burke ,  Toby H. Richardson ,  Paula M. Hicks

IPC分类号： C12N9/88 ,  C07H21/04 ,  C07H21/00 ,  C12N15/74 ,  C12N5/07 ,  C12N5/04 ,  C12N1/15 ,  C12N1/21 ,  A01K67/033 ,  A01H5/00 ,  C12N9/24 ,  C07K16/00 ,  C12P21/00 ,  C07K7/08 ,  C07K14/00 ,  A61K38/47 ,  C12P1/00 ,  C12P13/14 ,  C07C205/01 ,  A61P35/00 ,  A23K1/00

CPC分类号： C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

摘要翻译： 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可以用于各种药物，农业和工业环境。 在一些实施方案中，本发明提供了可用于制备R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和莫纳甜的某些立体异构体的多肽和生物合成途径，例如R ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的某些立体异构体，例如R，R和S，R构型及其盐。