PROCEDE POUR LA DETERMINATION D'ANTIBIOTIQUES A NOYAU BETA-LACTAME DANS UN LIQUIDE BIOLOGIQUE
    3.
    发明授权
    PROCEDE POUR LA DETERMINATION D'ANTIBIOTIQUES A NOYAU BETA-LACTAME DANS UN LIQUIDE BIOLOGIQUE 有权
    程序来确定β-内酰胺核含抗生素

    公开(公告)号:EP1082451B1

    公开(公告)日:2006-06-28

    申请号:EP99919158.8

    申请日:1999-03-30

    IPC分类号: C12Q1/00

    摘要: The invention concerns a method for detecting antibiotics with beta -lactam core in a biological fluid comprising the following steps: a) contacting a specific volume of said biological fluid with an amount of identifying agent and incubating the resulting mixture in conditions for forming a complex of the antibiotics possibly present in said biological fluid with the identifying agent; b) contacting the mixture resulting from step a) with at least a reference antibiotic immobilised on a support, in conditions for forming a complex of the reference antibiotic with the amount of identifying agent which has not reacted in step a); and c) determining the amount of identifying agent fixed on the support. The invention is characterised in that the identifying agent comprises a receptor sensitive to antibiotics with beta -lactam core obtained from Bacillus licheniformis.

    MICROBIAL GROWTH DETECTOR
    5.
    发明授权
    MICROBIAL GROWTH DETECTOR 有权
    微生物生长检测器

    公开(公告)号:EP2566948B1

    公开(公告)日:2017-11-22

    申请号:EP10851126.2

    申请日:2010-12-23

    IPC分类号: C12M1/34

    摘要: The disclosure generally relates to a test device that detects microorganism growth by detecting a gas metabolite (e.g., carbon dioxide) produced during the growth of bacteria or other microorganism in a tested sample. The test device can contain a culture growth media separated from a detection area by a gas-permeable membrane. The gas-permeable membrane permits carbon dioxide to permeate into the detection area. The detection area includes a solidified mixture of pH indicators and a gelling agent in the form of a semi-permeable matrix. The optical properties, including the absorbance of light at various wavelengths, of the detection solution change with alterations in carbon dioxide concentration. This test device can then be placed in an incubation and optical detection instrument to monitor changes in optical properties of the detection are induced during microorganism growth in the culture medium.

    AUTOMATED PRESERVATIVE EFFICACY TEST METHOD AND DEVICE
    6.
    发明公开
    AUTOMATED PRESERVATIVE EFFICACY TEST METHOD AND DEVICE 审中-公开
    VERFAHREN UND VORRICHTUNGFÜRAUTOMATISIERTENPRÄSERVATIVWIRKSAMKEITSTEST

    公开(公告)号:EP3146063A1

    公开(公告)日:2017-03-29

    申请号:EP14892382.4

    申请日:2014-05-22

    发明人: EDEN, Gideon

    摘要: A preservative efficacy test for determining efficiency of at least one preservative material that is present in a consumable product to reduce or eliminate living microorganisms that includes challenging the preservative material with at least one microorganism derived from a pure culture by inoculating a sample of the product with a quantity of at least one challenge microorganism and allowing the microorganisms to grow for an predetermined interval after which the preservative is neutralized and a portion of the resulting sample is cultured in a testing system that includes at least one testing container, wherein the testing container contains liquid growth media and sensor means capable of monitoring microorganism growth by sensing the metabolic by-products generated by growth of microorganisms present in the liquid growth media and producing an output signal that can be measured and analyzed.

    摘要翻译: 用于确定存在于消耗品中的至少一种防腐材料的效率的防腐效力测试,以减少或消除活的微生物,其包括用至少一种来自纯培养物的微生物挑选所述防腐材料,所述微生物通过用 至少一种挑战微生物,并允许微生物生长预定间隔,之后将防腐剂中和,并将所得样品的一部分培养在包括至少一个测试容器的测试系统中,其中测试容器包含 液体生长培养基和传感器装置,其能够通过感测存在于液体生长培养基中的微生物生长产生的代谢副产物来监测微生物生长,并产生可被测量和分析的输出信号。

    CHROMOGENIC SUBSTRATE WITH A PH INDICATOR DYE
    7.
    发明授权
    CHROMOGENIC SUBSTRATE WITH A PH INDICATOR DYE 有权
    WITH pH指示剂染料显色底物

    公开(公告)号:EP1618377B1

    公开(公告)日:2011-07-27

    申请号:EP04749731.8

    申请日:2004-04-02

    发明人: MAYER, Brent, A.

    IPC分类号: G01N33/53 C12Q1/28

    摘要: A composition for an enzyme-linked assay is described which comprises a chromogenic substrate that produces a detectable color with a maximum absorbance at one wavelength in reactions of the assay which contain the enzyme and a pH indicator dye that produces a detectable color with a maximum absorbance at a second wavelength when the pH of the reactions of the assay is changed to terminate the reactions. The chromogenic substrate enables colorimetric detection of the bindable substance in positive reactions of the assay and the pH indicator dye enables negative reactions of the assay to be colorimetrically distinguished from false negative 3,3',5,5'-tetramethylbenzidine (TMB) and the pH indicator dye is preferably selected from the group consisting of cresol red and m-cresol purple.

    CHROMOGENIC SUBSTRATE WITH A PH INDICATOR DYE
    10.
    发明公开
    CHROMOGENIC SUBSTRATE WITH A PH INDICATOR DYE 有权
    WITH pH指示剂染料显色底物

    公开(公告)号:EP1618377A2

    公开(公告)日:2006-01-25

    申请号:EP04749731.8

    申请日:2004-04-02

    发明人: MAYER, Brent, A.

    IPC分类号: G01N33/53 C12Q1/28

    摘要: A composition for an enzyme-linked assay is described which comprises a chromogenic substrate that produces a detectable color with a maximum absorbance at one wavelength in reactions of the assay which contain the enzyme and a pH indicator dye that produces a detectable color with a maximum absorbance at a second wavelength when the pH of the reactions of the assay is changed to terminate the reactions. The chromogenic substrate enables colorimetric detection of the bindable substance in positive reactions of the assay and the pH indicator dye enables negative reactions of the assay to be colorimetrically distinguished from false negative 3,3',5,5'-tetramethylbenzidine (TMB) and the pH indicator dye is preferably selected from the group consisting of cresol red and m-cresol purple.