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    AUTOMATED PRESERVATIVE EFFICACY TEST METHOD AND DEVICE
    8.
    发明公开
    AUTOMATED PRESERVATIVE EFFICACY TEST METHOD AND DEVICE 审中-公开
    VERFAHREN UND VORRICHTUNGFÜRAUTOMATISIERTENPRÄSERVATIVWIRKSAMKEITSTEST

    公开(公告)号：EP3146063A1

    公开(公告)日：2017-03-29

    申请号：EP14892382.4

    申请日：2014-05-22

    申请人： NEOGEN CORPORATION ,  Eden, Gideon

    发明人： EDEN, Gideon

    IPC分类号： C12Q1/02 ,  C12Q1/06 ,  C12M1/34 ,  C12R1/66 ,  C12R1/19 ,  C12R1/385 ,  C12R1/445

    CPC分类号： G01N35/00693 ,  C12Q1/025 ,  C12Q1/06 ,  C12Q1/18 ,  G01N2035/00702

    摘要： A preservative efficacy test for determining efficiency of at least one preservative material that is present in a consumable product to reduce or eliminate living microorganisms that includes challenging the preservative material with at least one microorganism derived from a pure culture by inoculating a sample of the product with a quantity of at least one challenge microorganism and allowing the microorganisms to grow for an predetermined interval after which the preservative is neutralized and a portion of the resulting sample is cultured in a testing system that includes at least one testing container, wherein the testing container contains liquid growth media and sensor means capable of monitoring microorganism growth by sensing the metabolic by-products generated by growth of microorganisms present in the liquid growth media and producing an output signal that can be measured and analyzed.

    摘要翻译： 用于确定存在于消耗品中的至少一种防腐材料的效率的防腐效力测试，以减少或消除活的微生物，其包括用至少一种来自纯培养物的微生物挑选所述防腐材料，所述微生物通过用 至少一种挑战微生物，并允许微生物生长预定间隔，之后将防腐剂中和，并将所得样品的一部分培养在包括至少一个测试容器的测试系统中，其中测试容器包含 液体生长培养基和传感器装置，其能够通过感测存在于液体生长培养基中的微生物生长产生的代谢副产物来监测微生物生长，并产生可被测量和分析的输出信号。

    METHOD FOR THE MICROBIOLOGICAL DETERMINATION OF TRACES OF ANTIBIOTICS IN LOW VOLUME BIOLOGICAL SAMPLES
    9.
    发明公开
    METHOD FOR THE MICROBIOLOGICAL DETERMINATION OF TRACES OF ANTIBIOTICS IN LOW VOLUME BIOLOGICAL SAMPLES 审中-公开
    方法抗生素的痕量在低体积生物样品微生物学测定

    公开(公告)号：EP3085790A1

    公开(公告)日：2016-10-26

    申请号：EP14872796.9

    申请日：2014-12-11

    申请人： Universidad De Santiago De Chile

    发明人： SANHUEZA CHAVEZ, Loreto ,  WILKENS ANWANDTER, Marcela Andrea ,  LAURIDO FUENZALIDA, Claudio Aurelio

    IPC分类号： C12Q1/02 ,  C12Q1/18 ,  C12R1/445

    CPC分类号： C12Q1/18 ,  G01N2333/31

    摘要： A microbiological method for determining traces of antibiotics in low volume biological samples, which comprises determining the minimum inhibitory concentration (MIC) of an antibiotic selected from a group consisting of minocycline, ciprofloxacin, kanamycin, tetracycline and oxacillin against Staphylococcus aureus. The bacteria are grown overnight in Mueller-Hinton broth (MHB), said cultures subsequently being diluted to 0.5 McFarland standard (1.5 x 108 cells/mL). Said antibiotics are then added to 96-well plates to a final volume of 200 µL and incubated at 37°C for 18 hours. Absorbency is determined and the results are expressed as the percentage of inhibition relative to the control containing the biological sample, selected in turn from urine, blood, synovial fluid, eye aqueous humour or cerebrospinal fluid (CSF), and the bacterial culture. The curve representing the percentage of bacterial growth inhibition versus concentration of antibiotic is obtained, and fitted by "Four Parameter Logistic Curve (4PL)" analysis, yielding the antibiotic concentration values in the biological sample. The method allows to determine amounts of antibiotic in the biological sample even at ng/mL level.

    摘要翻译： 对于低体积的生物样品中的抗生素确定性采矿迹线的微生物学方法，该方法包括确定性采矿从包括米诺环素，环丙沙星，卡那霉素，四环素和苯唑西林对金黄色葡萄球菌的选择的抗生素的最小抑制浓度（MIC）。 该细菌在Mueller Hinton肉汤（MHB）中生长过夜，说培养随后稀释至0.5麦氏标准（1.5×108细胞/ mL）。 然后，所述抗生素加入到96孔板中，以200μl的最终体积，并在37℃下温育18小时。 吸收能力被确定性开采并且将结果表示为相对于抑制的百分比包含所述生物样品，依次从尿，血液，滑液，眼睛wässrige幽默或脑脊髓液（CSF）中选择的控制，和细菌培养。 代表的细菌生长抑制与抗生素的浓度的百分比的曲线被获得，并且通过“四参数逻辑曲线（4PL）”分析嵌合，在生物样品中得到的抗菌素的浓度值。 该方法允许甚至在毫微克/毫升的水平的生物样品中的抗生素的确定性矿量。