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    PRODUCTION OF BETA-LACTAM ANTIBIOTICS
    1.
    发明授权
    PRODUCTION OF BETA-LACTAM ANTIBIOTICS 有权
    产生β-内酰胺类抗生素

    公开(公告)号：EP2084270B1

    公开(公告)日：2013-11-20

    申请号：EP07820842.8

    申请日：2007-10-02

    申请人： DSM Sinochem Pharmaceuticals Netherlands B.V.

    发明人： HANS, Marcus ,  BOVENBERG, Roelof, Ary, Lans ,  KLAASSEN, Paul ,  LAAN, VAN DER, Jan Metske ,  BOER, Rémon

    IPC分类号： C12N9/02 ,  C12P37/00

    CPC分类号： C12N9/0004

    摘要： The present invention describes a process for the production of an N-α-amino-hydroxyphenylacetyl or an N-α-aminophenylacetyl &bgr;-lactam antibiotic comprising an IPNS-catalysed conversion of a precursor tripeptide hydroxyphenylglycyl-cysteinyl-valine (HpgCV) or phenylglycyl-cysteinyl-valine (PgCV), respectively, to the N-hydroxyphenylglycyl or the N-phenylglycyl &bgr;-lactam antibiotic, respectively. The tripeptide HpgCV or the tripeptide PgCV may further be prepared by contacting the amino acids hydroxyphenylglycine (Hpg) or phenylglycine (Pg), cystein (C) and valine (V) with a non-ribosomal peptide synthetase (NRPS) to effect formation of the tripeptide HpgCV or the tripeptide PgCV, the NRPS comprising a first module M1 specific for Hpg or Pg, a second module M2 specific for C and a third module M3 specific for V An IPNS is further provided having an improved activity in this conversion, as well as an NRPS catalysing the formation of the tripeptides. Also a host cell is provided capable of fermentatively producing &bgr;-lactam antibiotics with N-α-amino-hydroxyphenylacetyl or an N-α-aminophenylacetyl side chains.