公开(公告)号：EP0987332B1

公开(公告)日：2009-08-19

申请号：EP98914111.4

申请日：1998-04-20

申请人： KANEKA CORPORATION

发明人： YAMADA, Yukio ,  IWASAKI, Akira ,  KIZAKI, Noriyuki ,  MATSUMOTO, Keiji ,  IKENAKA, Yasuhiro ,  OGURA, Masahiro ,  HASEGAWA, Junzo

IPC分类号： C12P13/04 ,  C12N15/54

CPC分类号： C12P13/001 ,  C12N9/1096

摘要： A process for efficiently producing optically active amino compounds, mainly (R)-amino compounds, at a low cost by means of a microbial enzyme; a polypeptide suitable for use in the production process and having a stereoselective transaminase activity; and a DNA coding for the polypeptide. The process is characterized by comprising causing a transaminase to act on a ketone compound as an amino acceptor in the presence of a primary amine as an amino donor to stereoselectively transfer the amino group to thus obtain an optically active amino compound. The DNA contains a base sequence coding for a polypeptide having a stereoselective transaminase activity. The polypeptide can be obtained by incubating a microorganism belonging to the genus Arthrobacter.

公开(公告)号：EP1024139B1

公开(公告)日：2004-05-12

申请号：EP99935066.3

申请日：1999-08-05

申请人： KANEKA CORPORATION

发明人： KIZAKI, Noriyuki ,  YAMADA, Yukio ,  YASOHARA, Yoshihiko ,  NISHIYAMA, Akira ,  MIYAZAKI, Makoto ,  MITSUDA, Masaru ,  KONDO, Takeshi ,  UEYAMA, Noboru ,  INOUE, Kenji

IPC分类号： C07D319/06 ,  C07C67/343 ,  C07C67/31

CPC分类号： C07D319/06 ,  C07B2200/07 ,  C07C67/343 ,  C07C253/30 ,  C12P7/62 ,  C07C69/716 ,  C07C255/21

公开(公告)号：EP0857790A1

公开(公告)日：1998-08-12

申请号：EP96935392.9

申请日：1996-10-21

申请人： KANEKA CORPORATION

发明人： YAMADA, Yukio ,  IWASAKI, Akira ,  KIZAKI, Noriyuki ,  IKENAKA, Yasuhiro ,  OGURA, Masahiro ,  HASEGAWA, Junzo

IPC分类号： C12P13/00 ,  C12N9/10 ,  C12P41/00

CPC分类号： C12P13/001 ,  C12N9/1096 ,  C12P13/005 ,  C12P13/008 ,  C12P13/04 ,  C12P41/006 ,  Y10S435/83

摘要： A process for producing optically active (R)-amino compounds which comprises treating an aralkyl alkyl ketone compound (the amino receptor) coexisting with a racemic or (R)-amino compound (the amino donor) with an (R)-specific transaminase produced by a microorganism belonging to the genus Arthrobacter and thus effecting stereoselective transamination. This process makes it possible to facilitate the production in a high yield of optically active (R)-amino compounds, etc., having aryl, etc. at the 1-position which have been difficult to synthesize by the conventional techniques.

摘要翻译： 一种制备光学活性（R） - 氨基化合物的方法，其包括用（R） - 特异性转氨酶处理与外消旋或（R） - 氨基化合物（氨基供体）共存的芳烷基烷基酮化合物（氨基受体） 通过属于节杆菌属的微生物，从而实现立体选择性转氨酶。 该方法使得可以以高产率的方式促进在1-位上具有芳基等的光学活性（R） - 氨基化合物等的生产，这些常规技术难以合成。

公开(公告)号：EP0857790B1

公开(公告)日：2005-08-17

申请号：EP96935392.9

申请日：1996-10-21

申请人： KANEKA CORPORATION

发明人： YAMADA, Yukio ,  IWASAKI, Akira ,  KIZAKI, Noriyuki ,  IKENAKA, Yasuhiro ,  OGURA, Masahiro ,  HASEGAWA, Junzo

IPC分类号： C12P13/00 ,  C12N9/10 ,  C12P41/00

CPC分类号： C12P13/001 ,  C12N9/1096 ,  C12P13/005 ,  C12P13/008 ,  C12P13/04 ,  C12P41/006 ,  Y10S435/83

摘要： A process for producing optically active (R)-amino compounds which comprises treating an aralkyl alkyl ketone compound (the amino receptor) coexisting with a racemic or (R)-amino compound (the amino donor) with an (R)-specific transaminase produced by a microorganism belonging to the genus Arthrobacter and thus effecting stereoselective transamination. This process makes it possible to facilitate the production in a high yield of optically active (R)-amino compounds, etc., having aryl, etc. at the 1-position which have been difficult to synthesize by the conventional techniques.

公开(公告)号：EP1152054B1

公开(公告)日：2005-03-23

申请号：EP00977938.0

申请日：2000-11-24

申请人： Kaneka Corporation

发明人： KIZAKI, Noriyuki ,  YAMADA, Yukio ,  YASOHARA, Yoshihiko ,  HASEGAWA, Junzo

IPC分类号： C12N9/02 ,  C12N15/53 ,  C12N9/04 ,  C12N1/21 ,  C12P7/62

CPC分类号： C12N9/0006 ,  C12P7/62 ,  C12P41/00 ,  C12P41/002

摘要： A novel polypeptide capable of forming tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate; and a polynucleotide encoding the same. Namely, a polypeptide which has an enzymatic activity of assymetrically reducing tert-butyl (S)-6-chloro-5-hydroxy-3-oxohexanoate to give tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate and contains the amino acid sequence represented by SEQ ID NO:1 in Sequence Listing or an amino acid sequence derived from the above amino acid sequence by the substitution, insertion, deletion or addition of at lease one amino acid.

公开(公告)号：EP1024139A1

公开(公告)日：2000-08-02

申请号：EP99935066.3

申请日：1999-08-05

申请人： KANEKA CORPORATION

发明人： KIZAKI, Noriyuki ,  YAMADA, Yukio ,  YASOHARA, Yoshihiko ,  NISHIYAMA, Akira ,  MIYAZAKI, Makoto ,  MITSUDA, Masaru ,  KONDO, Takeshi ,  UEYAMA, Noboru ,  INOUE, Kenji

IPC分类号： C07D319/06 ,  C07D319/08 ,  C07C59/90 ,  C07C51/353 ,  C07C59/115 ,  C12P7/04

CPC分类号： C07D319/06 ,  C07B2200/07 ,  C07C67/343 ,  C07C253/30 ,  C12P7/62 ,  C07C69/716 ,  C07C255/21

摘要： This invention provides a process for producing optically active 2-[6-(hydroxymethyl)-1,3-dioxan-4-yl] acetic acid derivatives, which are of value as intermediates of drugs, from inexpensive starting materials without using any special equipment such as that required for super-low temperature reactions.
A process for producing optically active 2-[6-(hydroxymethyl)-1,3-dioxan-4-yl]acetic acid derivatives which comprises

reacting an acetic acid derivative at a temperature of not less than -30°C with an enolate prepared by permitting either a base or a metal having a valency of 0 to act on the derivative to produce a hydroxyoxohexanoic acid derivative,
reducing this compound with the aid of a strain of microorganism to provide a halomethyldioxanylacetic acid derivative,
treating this compound with an acetalizing agent in the presence of an acid catalyst to provide a halomethyldioxanylacetic acid derivative,
reacted with an acyloxylating agent to provide a acyloxymethyldioxanylacetic acid derivative, and
subjecting this compound to solvolysis in the presence of a base.

摘要翻译： 本发明提供了一种从廉价起始原料生产具有价值作为药物中间体的光学活性2-δ-（羟甲基）-1,3-二恶烷-4-基乙酸衍生物的方法，而不使用任何特殊设备，例如 这是超低温反应所需的。 一种制备光学活性的2-δ-（羟甲基）-1,3-二恶烷-4-基乙酸衍生物的方法，其包括使不饱和-30℃的乙酸衍生物与通过使任一种 碱或金属，其化合价为0，作用于衍生物以产生羟基己酸衍生物，借助微生物菌株还原该化合物，得到卤代甲基二恶烷基乙酸衍生物，在缩合剂存在下用缩醛化剂处理该化合物 的酸催化剂，得到卤代甲基二恶烷基乙酸衍生物，与酰氧基化剂反应，得到酰氧基甲基二恶烷基乙酸衍生物，并在碱的存在下使其溶解。

公开(公告)号：EP1045025B1

公开(公告)日：2008-10-22

申请号：EP99951134.8

申请日：1999-10-29

申请人： KANEKA CORPORATION

发明人： IWASAKI, Akira ,  YAMADA, Yukio ,  IKENAKA, Yasuhiro ,  YASOHARA, Yoshihiko ,  HASEGAWA, Junzo

IPC分类号： C12N9/10 ,  C12P41/00

CPC分类号： C12N9/1096 ,  C12P41/006

摘要： An enzyme capable of converting a ketone compound into an optically active amino compound via a transamination reaction and a process for producing an optically active amino compound by using this enzyme. (S)- alpha -Phenethylamine:pyruvate transaminase which acts on (S)- alpha -phenethylamine and a ketone compound and catalyzes a transamination reaction whereby acetophenone and an amino compound corresponding to the ketone compound are formed; a process for producing an optically active amino compound by using the transaminase; and a method for culturing a microorganism producing the above transaminase wherein at least one inducer selected from the group consisting of propylamine, 1-butylamine, 2-butylamine, 2-pentylamine, isopropylamine and isobutylamine is added to the medium in culturing the transaminase-producing microorganism.

摘要翻译： 能够通过转氨基反应将酮化合物转化为光学活性氨基化合物的酶和通过使用该酶生产光学活性氨基化合物的方法。 （S）-α-苯乙胺：丙酮酸转氨酶，其作用于（S）-α-苯乙胺和酮化合物并催化转氨反应，由此形成苯乙酮和对应于该酮化合物的氨基化合物; 通过使用转氨酶生产光学活性氨基化合物的方法; 以及用于培养产生上述转氨酶的微生物的方法，其中在培养产生转氨酶时将至少一种选自丙胺，1-丁胺，2-丁胺，2-戊胺，异丙胺和异丁胺的诱导剂加入培养基中 微生物。

公开(公告)号：EP1152054A1

公开(公告)日：2001-11-07

申请号：EP00977938.0

申请日：2000-11-24

申请人： Kaneka Corporation

发明人： KIZAKI, Noriyuki ,  YAMADA, Yukio ,  YASOHARA, Yoshihiko ,  HASEGAWA, Junzo

IPC分类号： C12N9/02 ,  C12N15/53 ,  C12N9/04 ,  C12N1/21 ,  C12P7/62

CPC分类号： C12N9/0006 ,  C12P7/62 ,  C12P41/00 ,  C12P41/002

摘要： A novel polypeptide for producing tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate, a gene encoding the polypeptide, and a method of using the polypeptide, are provided. The polypeptide has an enzyme activity to asymmetrically reduce tert-butyl (S)-6-chloro-5-hydroxy-3-oxohexanoate to tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate. The polypeptide comprises the amino acid sequence represented by SEQ ID NO. 1 in the Sequence Listing, or the amino acid sequence having at least one amino acid substitution, insertion, deletion, or addition thereof.

摘要翻译： 提供了一种用于制备（3R，5S）-6-氯-3,5-二羟基己酸叔丁酯，编码该多肽的基因以及使用该多肽的方法的新型多肽。 该多肽具有不对称地将（S）-6-氯-5-羟基-3-氧代己酸叔丁酯还原成（3R，5S）-6-氯-3,5-二羟基己酸叔丁酯的酶活性。 多肽包含SEQ ID NO：1所示的氨基酸序列。 或者具有至少一个氨基酸取代，插入，缺失或添加的氨基酸序列。

公开(公告)号：EP1045025A1

公开(公告)日：2000-10-18

申请号：EP99951134.8

申请日：1999-10-29

申请人： KANEKA CORPORATION

发明人： IWASAKI, Akira ,  YAMADA, Yukio ,  IKENAKA, Yasuhiro ,  YASOHARA, Yoshihiko ,  HASEGAWA, Junzo

IPC分类号： C12N9/10 ,  C12P41/00

CPC分类号： C12N9/1096 ,  C12P41/006

摘要： Relating to an enzyme capable of efficiently converting a ketone compound to an optically active amino compound by transamination, and a process for preparing an optically active amino compound using the enzyme. An (S)-α-phenethylamine : pyruvate transaminase, which acts on (S)-α-phenethylamine and a ketone compound, thereby catalyzing transamination for forming acetophenone and an amino compound corresponding to the ketone compound; a process for preparing an optically active amino compound using the transaminase; and a method for culturing a microorganism for producing the above transaminase, comprising adding to a medium one or more compounds selected from the group consisting of propylamine, 1-butylamine, 2-butylamine, 2-pentylamine, isopropylamine and isobutylamine as an inducer for the enzyme when a microorganism for producing (S)-α-phenethylamine : pyruvate transaminase is cultured.

摘要翻译： 关于能够通过转氨酶有效地将酮化合物转化为光学活性氨基化合物的酶，以及使用该酶制备光学活性氨基化合物的方法。 （S）-α-苯乙胺：丙酮酸转氨酶，其作用于（S）-α-苯乙胺和酮化合物，从而催化转氨酶形成苯乙酮和对应于酮化合物的氨基化合物; 使用转氨酶制备光学活性氨基化合物的方法; 以及培养用于制备上述转氨酶的微生物的方法，包括向培养基中加入一种或多种选自丙胺，1-丁胺，2-丁胺，2-戊胺，异丙胺和异丁胺的化合物作为诱导剂， 酶用于生产（S）-α-苯乙胺：丙酮酸转氨酶的微生物。

公开(公告)号：EP0987332A1

公开(公告)日：2000-03-22

申请号：EP98914111.4

申请日：1998-04-20

申请人： Kaneka Corporation

发明人： YAMADA, Yukio ,  IWASAKI, Akira ,  KIZAKI, Noriyuki ,  MATSUMOTO, Keiji ,  IKENAKA, Yasuhiro ,  OGURA, Masahiro ,  HASEGAWA, Junzo

IPC分类号： C12P13/04 ,  C12N15/54

CPC分类号： C12P13/001 ,  C12N9/1096

摘要： A process for efficiently producing optically active amino compounds, mainly (R)-amino compounds, at a low cost by means of a microbial enzyme; a polypeptide suitable for use in the production process and having a stereoselective transaminase activity; and a DNA coding for the polypeptide. The process is characterized by comprising causing a transaminase to act on a ketone compound as an amino acceptor in the presence of a primary amine as an amino donor to stereoselectively transfer the amino group to thus obtain an optically active amino compound. The DNA contains a base sequence coding for a polypeptide having a stereoselective transaminase activity. The polypeptide can be obtained by incubating a microorganism belonging to the genus Arthrobacter .

摘要翻译： 一种用于有效地制造光学活性氨基化合物，主要（R）的氨基化合物，在通过微生物酶的手段低成本过程; 适合于在生产过程中使用，并且具有立体选择性转氨酶活性的多肽; 和DNA编码的多肽。 该过程由包括使转氨酶在伯胺的存在下在酮化合物作为氨基受体的作为氨基供体的立体选择性地转移的氨基，得到因而光学活性氨基化合物为特征的。 的DNA含有的碱基序列编码具有立体选择性转氨酶活性的多肽。 所述多肽可通过温育属于节杆菌属的微生物来获得。