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11 条记录 (耗时 0.012 秒)

    BIOMOLECULE ANALYSIS KIT AND BIOMOLECULE ANALYSIS METHOD
    4.
    发明公开
    BIOMOLECULE ANALYSIS KIT AND BIOMOLECULE ANALYSIS METHOD 审中-公开

    公开(公告)号：EP3584306A1

    公开(公告)日：2019-12-25

    申请号：EP19190779.9

    申请日：2015-02-02

    申请人： Toppan Printing Co., Ltd.

    发明人： MAKINO, Yoichi ,  KUNITOMI, Tomoko

    IPC分类号： C12M1/34 ,  C12Q1/48 ,  B01L3/00 ,  C12Q1/68 ,  G01N21/64

    摘要： A biomolecule analysis kit includes a reaction container configured to perform an enzymatic reaction, the reaction container including a base portion which has a container-shaped portion and a low-adsorption structural portion which is provided on at least the inner surface of the container-shaped portion, the low-adsorption structural portion having an adsorption rate lower than the base portion at which at least one of a sample which becomes a target of analysis in the enzymatic reaction and a reagent for the enzymatic reaction is adsorbed thereonto, wherein a signal resulting from the enzymatic reaction is configured to be detected when the enzymatic reaction is performed in the reaction container.

    METHOD FOR DETECTING TARGET BASE SEQUENCE USING COMPETITIVE PRIMER
    5.
    发明公开
    METHOD FOR DETECTING TARGET BASE SEQUENCE USING COMPETITIVE PRIMER 有权
    VERFAHREN ZUR ERKENNUNG VON ZIELBASISSEQUENZEN MITHILFE KONKURRIERENDER PRIMER

    公开(公告)号：EP2551356A1

    公开(公告)日：2013-01-30

    申请号：EP11759408.5

    申请日：2011-03-23

    申请人： Toppan Printing Co., Ltd.

    发明人： MAKINO, Yoichi ,  YAMANE, Akio ,  NAKAYAMA, Masato

    IPC分类号： C12Q1/68 ,  C12N15/09

    CPC分类号： C12Q1/6869 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2531/119 ,  C12Q2537/161 ,  C12Q2531/143 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2525/185 ,  C12Q2525/204 ,  C12Q2535/125 ,  C12Q2549/101 ,  C12Q2563/107 ,  C12Q2565/101

    摘要： Disclosed is a method of detecting a target base sequence having a polymorphic base, the method including: (a) a step of adding to a nucleic acid sample having a target nucleic acid that includes a base sequence including the target base sequence: at least one type of detection primer, at least one type of competitive primer, and at least one type of common primer; (b) a step of annealing the detection primer and the competitive primer to the target nucleic acid in a competitive manner, thereby synthesizing an extension product A; (c) a step of annealing the common primer to the extension product A obtained in the step (b) or in the following step (d), thereby synthesizing an extension product B; (d) a step of annealing the detection primer or the competitive primer to the extension product B obtained in the previous step (c), thereby synthesizing the extension product A; and (e) a step of detecting the extension product A or the extension product B.

    摘要翻译： 本发明公开了一种检测具有多态碱基的目标碱基序列的方法，该方法包括：（a）向具有包含目标碱基序列的碱基序列的靶核酸的核酸样品中添加至少一种 检测引物的类型，至少一种类型的竞争性引物和至少一种类型的共同引物; （b）以竞争的方式将检测引物和竞争性引物退火至靶核酸，由此合成延伸产物A; （c）将通用引物退火到步骤（b）或后续步骤（d）中获得的延伸产物A的步骤，从而合成延伸产物B; （d）将检测引物或竞争性引物退火到在前面步骤（c）中获得的延伸产物B的步骤，从而合成延伸产物A; 和（e）检测扩展产品A或扩展产品B的步骤。

    METHOD AND KIT FOR TARGET MOLECULE DETECTION
    6.
    发明公开
    METHOD AND KIT FOR TARGET MOLECULE DETECTION 审中-公开

    公开(公告)号：EP4047368A1

    公开(公告)日：2022-08-24

    申请号：EP22160097.6

    申请日：2017-05-19

    申请人： Toppan Printing Co., Ltd.

    发明人： MAKINO, Yoichi ,  HIRASE, Takumi ,  ARAI, Noriaki ,  NOJI, Hiroyuki

    IPC分类号： G01N33/543 ,  C12Q1/68 ,  G01N33/58

    摘要： A method for detecting a target molecule includes a step of preparing a plurality of types of specific binding substances that are labeled with a plurality of nucleic acid fragments each having a labeling substance that generates a fluorescence signal of a different fluorescence wavelength, and that each recognize a different epitope, and preparing a plurality of types of first target molecules; a step of binding the plurality of types of specific binding substances to the plurality of types of first target molecules; a step of respectively detecting the plurality of types of specific binding substances; and a step of determining the plurality of types of first target molecules by a plurality of the fluorescence signals corresponding to the plurality of types of specific binding substances.

    MICROFLUIDIC DEVICE AND SAMPLE ANALYSIS METHOD
    7.
    发明公开
    MICROFLUIDIC DEVICE AND SAMPLE ANALYSIS METHOD 审中-公开

    公开(公告)号：EP3933412A1

    公开(公告)日：2022-01-05

    申请号：EP20767115.7

    申请日：2020-02-19

    申请人： Toppan Printing Co., Ltd.

    发明人： GOTO, Keisuke ,  MAKINO, Yoichi ,  SUZUKI, Yuta

    IPC分类号： G01N37/00 ,  C12M1/00 ,  G01N35/08

    摘要： A microfluidic device (1) including: a microwell array (30) having a plurality of microwells; and a cover member (20) facing the microwell array (30) with a gap therebetween, wherein a flow path is provided between the microwell array (30) and the cover member (20), and a surface (20a) of the cover member (20) facing the microwell array (30) has an arithmetic average roughness Ra of 70 nm or less, and a sample analysis method using the microfluidic device (1).

    TARGET MOLECULE DETECTION METHOD
    8.
    发明公开
    TARGET MOLECULE DETECTION METHOD 审中-公开

    公开(公告)号：EP3712275A1

    公开(公告)日：2020-09-23

    申请号：EP18879365.7

    申请日：2018-11-15

    申请人： Toppan Printing Co., Ltd.

    发明人： HIRASE, Takumi ,  MAKINO, Yoichi ,  HORIUCHI, Yosuke

    IPC分类号： C12Q1/02 ,  G01N33/50 ,  G01N33/53 ,  G01N33/68 ,  C12M1/34

    摘要： A method for detecting a target molecule includes the steps of: providing a fluid device having a substrate and a well array disposed on the substrate; supplying a dispersion liquid of a structure including a target molecule into the well array of the fluid device, and introducing the structure into wells of the well array; supplying a sealing solution into the well array, forming a layer of the sealing solution on the dispersion liquid introduced in the wells, and sealing the dispersion liquid in the wells; extracting the target molecule from the structure; and detecting the target molecule.

    MICROFLUIDIC DEVICE AND SAMPLE ANALYSIS METHOD
    10.
    发明公开
    MICROFLUIDIC DEVICE AND SAMPLE ANALYSIS METHOD 审中-公开

    公开(公告)号：EP3933411A1

    公开(公告)日：2022-01-05

    申请号：EP20763053.4

    申请日：2020-02-19

    申请人： Toppan Printing Co., Ltd.

    发明人： GOTO, Keisuke ,  MAKINO, Yoichi

    IPC分类号： G01N37/00 ,  C12M1/00 ,  C12M1/34 ,  G01N35/08 ,  G01N35/10

    摘要： There is provided a microfluidic device (1) including a flow path (35), and one or more droplet holders (11) connected to the flow path (35). In the microfluidic device (1), the flow path (35) has a height of more than 0 µm and 30 µm or less. There is also provided a sample analysis method that uses the microfluidic device (1). The method includes introducing a sample-containing aqueous solution into the flow path and allowing the droplet holders to accommodate the aqueous solution, introducing a sealant into the flow path for replacement with the aqueous solution present in the flow path and encapsulating the aqueous solution in the droplet holders, causing a reaction in the droplet holders and generating signals for detection, and detecting the signals.