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公开(公告)号：EP2870239B1
公开(公告)日：2018-10-31
申请号：EP13734062.6
申请日：2013-07-03
申请人： Wintershall Holding GmbH , BASF SE
发明人： BROCKMANN, Beata , HEROLD, Andrea , ZELDER, Oskar , HAEFNER, Stefan , FLECK, Christian , SCHRÖDER, Hartwig , GRANSTRÖM, Mari , SCHMIDT, Julia, Kristiane
CPC分类号： C12P19/04 , C12N9/1051 , C12Y204/01034
摘要： The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-&bgr;-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity or the use of such a polypeptide for producing &bgr;-glucans. Furthermore, the present invention relates to methods for producing &bgr;-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity into a microorganism being able to synthesize &bgr;-glucans.
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2.发明公开GENETICALLY MODIFIED MICROORGANISMS CAPABLE OF PRODUCING BETA-GLUCANS AND METHODS FOR PRODUCING BETA-GLUCANS 审中-公开基因修饰的β-葡聚糖的微生物及其制备方法β-葡聚糖
公开(公告)号：EP2870239A1
公开(公告)日：2015-05-13
申请号：EP13734062.6
申请日：2013-07-03
申请人： Wintershall Holding GmbH , BASF SE
发明人： BROCKMANN, Beata , HEROLD, Andrea , ZELDER, Oskar , HAEFNER, Stefan , FLECK, Christian , SCHRÖDER, Hartwig , GRANSTRÖM, Mari , SCHMIDT, Julia, Kristiane
CPC分类号： C12P19/04 , C12N9/1051 , C12Y204/01034
摘要： The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-&bgr;-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity or the use of such a polypeptide for producing &bgr;-glucans. Furthermore, the present invention relates to methods for producing &bgr;-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity into a microorganism being able to synthesize &bgr;-glucans.
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公开(公告)号：EP3227451A1
公开(公告)日：2017-10-11
申请号：EP15804453.7
申请日：2015-12-02
发明人： ROLLIE, Sascha , KAEPPLER, Tobias , SCHWALB, Carsten , LEHR, Florian , HLUBEK, Andrea , THERRE, Joerg , FLECK, Christian , LETZELTER, Thomas , FREYER, Stephan , HEROLD, Andrea , RETTENMAIER, Hansjoerg , LEONHARDT, Bernd
CPC分类号： C12P19/04 , B01D61/147 , B01D2315/10 , C08B37/0024
摘要： The presently claimed invention is directed to a method for the preparation of an aqueous solution comprising at least one beta-glucan comprising at least the steps of a) fermentation of at least one fungal strain in an a fermentation broth, b) addition of at least one acid to the fermentation broth to adjust the pH to a value in the range of ≧2.0 to ≦4.0 and c) filtration of the fermentation broth to obtain an aqueous solution comprising the at least one beta-glucan and at least one beta-glucan that is obtained by this method.
摘要翻译： 本发明涉及制备包含至少一种β-葡聚糖的水溶液的方法，所述水溶液包含至少以下步骤：a）在发酵液中发酵至少一种真菌菌株，b）至少添加 将一种酸用于发酵液以将pH值调节至≥2.0至≤4.0的范围内的值，和c）过滤发酵液以获得包含至少一种β-葡聚糖和至少一种β-葡聚糖的水溶液 这是通过这种方法获得的。
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4.发明公开PROCESS FOR PRODUCING A BETA-GLUCAN POLYMER AND GENETICALLY MODIFIED MICROORGANISMS USEFUL IN THIS PROCESS 审中-公开生产β-葡聚糖聚合物和本方法中有用的遗传修饰的微生物的方法
公开(公告)号：EP3083951A1
公开(公告)日：2016-10-26
申请号：EP14830519.6
申请日：2014-12-12
CPC分类号： C12N9/1051 , C12N9/107 , C12N9/1205 , C12N9/1241 , C12N9/90 , C12P19/04
摘要： Process for producing a polymer consisting of a linear main chain of beta-D-(1-3)- glucopyranosyl units having a single beta-D-glucopyranosyl unit (1-6) linked to a beta-D- glucopyranosyl unit of the linear main chain with an average branching degree of about 0.3, said process comprising the steps of: (i) culturing in a medium a genetically modified microorganism capable of producing a polymer consisting of a linear main chain of beta-D-(1-3)-glucopyranosyl units having a single beta-D-glucopyranosylunit (1-6) linked to a beta-D-glucopyranosyl unit of the linear main chain with an average branching degree of about 0.3, wherein the modification confers an increased activity -compared to a non-modified control microorganism of the same strain -of at least two gene products selected from the group consisting of (a) hexokinase, (b) phosphoglucomutase,. (c) UTP-glucose-1-phosphate uridyltrasferase, (d) 1,3–beta-glucan synthase (e) branching enzyme; under conditions allowing said microorganism to produce said polymer; (ii) optionally recovering said polymer from the medium.
摘要翻译： 用于生产由β-D-（1-3） - 吡喃葡萄糖基单元的线性主链组成的聚合物的方法，所述β-D-（1-3） - 吡喃葡萄糖基单元具有与线性的β-D-吡喃葡萄糖基单元连接的单个β-D-吡喃葡萄糖基单元 主链的平均支化度为约0.3，所述方法包括以下步骤：（i）在培养基中培养能够产生由β-D-（1-3）的线性主链组成的聚合物的基因修饰微生物， - 吡喃葡萄糖基单元，其具有与平均支化度为约0.3的线性主链的β-D-吡喃葡萄糖基单元连接的单一β-D-吡喃葡萄糖基单元（1-6），其中所述修饰赋予与 （a）己糖激酶，（b）磷酸葡萄糖变位酶，至少两种基因产物的相同菌株的未修饰的对照微生物。 （c）UTP-葡萄糖-1-磷酸尿苷转移酶，（d）1,3-β-葡聚糖合酶（e）分支酶; 在允许所述微生物产生所述聚合物的条件下; （ii）任选从介质中回收所述聚合物。
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