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    PROCESS FOR PRODUCING A BETA-GLUCAN POLYMER AND GENETICALLY MODIFIED MICROORGANISMS USEFUL IN THIS PROCESS
    4.
    发明公开
    PROCESS FOR PRODUCING A BETA-GLUCAN POLYMER AND GENETICALLY MODIFIED MICROORGANISMS USEFUL IN THIS PROCESS 审中-公开
    生产β-葡聚糖聚合物和本方法中有用的遗传修饰的微生物的方法

    公开(公告)号：EP3083951A1

    公开(公告)日：2016-10-26

    申请号：EP14830519.6

    申请日：2014-12-12

    申请人： Wintershall Holding GmbH

    发明人： HLUBEK, Andrea ,  BROCKMANN, Beata ,  HEROLD, Andrea ,  GRANSTRÖM, Mari ,  FLECK, Christian

    IPC分类号： C12N9/10 ,  C12P19/04

    CPC分类号： C12N9/1051 ,  C12N9/107 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/90 ,  C12P19/04

    摘要： Process for producing a polymer consisting of a linear main chain of beta-D-(1-3)- glucopyranosyl units having a single beta-D-glucopyranosyl unit (1-6) linked to a beta-D- glucopyranosyl unit of the linear main chain with an average branching degree of about 0.3, said process comprising the steps of: (i) culturing in a medium a genetically modified microorganism capable of producing a polymer consisting of a linear main chain of beta-D-(1-3)-glucopyranosyl units having a single beta-D-glucopyranosylunit (1-6) linked to a beta-D-glucopyranosyl unit of the linear main chain with an average branching degree of about 0.3, wherein the modification confers an increased activity -compared to a non-modified control microorganism of the same strain -of at least two gene products selected from the group consisting of (a) hexokinase, (b) phosphoglucomutase,. (c) UTP-glucose-1-phosphate uridyltrasferase, (d) 1,3–beta-glucan synthase (e) branching enzyme; under conditions allowing said microorganism to produce said polymer; (ii) optionally recovering said polymer from the medium.

    摘要翻译： 用于生产由β-D-（1-3） - 吡喃葡萄糖基单元的线性主链组成的聚合物的方法，所述β-D-（1-3） - 吡喃葡萄糖基单元具有与线性的β-D-吡喃葡萄糖基单元连接的单个β-D-吡喃葡萄糖基单元 主链的平均支化度为约0.3，所述方法包括以下步骤：（i）在培养基中培养能够产生由β-D-（1-3）的线性主链组成的聚合物的基因修饰微生物， - 吡喃葡萄糖基单元，其具有与平均支化度为约0.3的线性主链的β-D-吡喃葡萄糖基单元连接的单一β-D-吡喃葡萄糖基单元（1-6），其中所述修饰赋予与 （a）己糖激酶，（b）磷酸葡萄糖变位酶，至少两种基因产物的相同菌株的未修饰的对照微生物。 （c）UTP-葡萄糖-1-磷酸尿苷转移酶，（d）1,3-β-葡聚糖合酶（e）分支酶; 在允许所述微生物产生所述聚合物的条件下; （ii）任选从介质中回收所述聚合物。

    METHOD OF REDUCING GENE EXPRESSION USING MODIFIED CODON USAGE
    10.
    发明公开
    METHOD OF REDUCING GENE EXPRESSION USING MODIFIED CODON USAGE 有权
    程序基因表达的修饰密码子使用的减少

    公开(公告)号：EP2082045A1

    公开(公告)日：2009-07-29

    申请号：EP07821516.7

    申请日：2007-10-18

    申请人： BASF SE

    发明人： HEROLD, Andrea ,  KLOPPROGGE, Corinna ,  SCHRÖDER, Hartwig ,  ZELDER, Oskar ,  JEONG, Weol Kyu

    IPC分类号： C12N15/77

    CPC分类号： C12N15/77

    摘要： The present invention is directed to a method of reducing the amount of at least one polypeptide in a host cell by expressing a nucleotide sequence encoding for the polypeptide in the host cell wherein the nucleotide sequence uses codons that are rarely used according to the codon usage of the host organism. Furthermore, the present invention relates to nucleotide sequences encoding for a polypeptide with a codon usage that has been adjusted to use codons that are only rarely used according to the codon usage of the host organism. The present invention further relates to the use of such sequences and methods for producing fine chemicals such as amino acids, sugars, lipids, oils, carbohydrates, vitamins, cofactors etc.