公开(公告)号：EP2870239B1

公开(公告)日：2018-10-31

申请号：EP13734062.6

申请日：2013-07-03

申请人： Wintershall Holding GmbH ,  BASF SE

发明人： BROCKMANN, Beata ,  HEROLD, Andrea ,  ZELDER, Oskar ,  HAEFNER, Stefan ,  FLECK, Christian ,  SCHRÖDER, Hartwig ,  GRANSTRÖM, Mari ,  SCHMIDT, Julia, Kristiane

IPC分类号： C12N9/10 ,  C12P19/04

CPC分类号： C12P19/04 ,  C12N9/1051 ,  C12Y204/01034

摘要： The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-&bgr;-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity or the use of such a polypeptide for producing &bgr;-glucans. Furthermore, the present invention relates to methods for producing &bgr;-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity into a microorganism being able to synthesize &bgr;-glucans.

公开(公告)号：EP2870239A1

公开(公告)日：2015-05-13

申请号：EP13734062.6

申请日：2013-07-03

申请人： Wintershall Holding GmbH ,  BASF SE

发明人： BROCKMANN, Beata ,  HEROLD, Andrea ,  ZELDER, Oskar ,  HAEFNER, Stefan ,  FLECK, Christian ,  SCHRÖDER, Hartwig ,  GRANSTRÖM, Mari ,  SCHMIDT, Julia, Kristiane

IPC分类号： C12N9/10 ,  C12P19/04

CPC分类号： C12P19/04 ,  C12N9/1051 ,  C12Y204/01034

摘要： The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-&bgr;-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity or the use of such a polypeptide for producing &bgr;-glucans. Furthermore, the present invention relates to methods for producing &bgr;-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-&bgr;-D-glucan synthase-activity into a microorganism being able to synthesize &bgr;-glucans.

公开(公告)号：EP3083951A1

公开(公告)日：2016-10-26

申请号：EP14830519.6

申请日：2014-12-12

申请人： Wintershall Holding GmbH

发明人： HLUBEK, Andrea ,  BROCKMANN, Beata ,  HEROLD, Andrea ,  GRANSTRÖM, Mari ,  FLECK, Christian

IPC分类号： C12N9/10 ,  C12P19/04

CPC分类号： C12N9/1051 ,  C12N9/107 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/90 ,  C12P19/04

摘要： Process for producing a polymer consisting of a linear main chain of beta-D-(1-3)- glucopyranosyl units having a single beta-D-glucopyranosyl unit (1-6) linked to a beta-D- glucopyranosyl unit of the linear main chain with an average branching degree of about 0.3, said process comprising the steps of: (i) culturing in a medium a genetically modified microorganism capable of producing a polymer consisting of a linear main chain of beta-D-(1-3)-glucopyranosyl units having a single beta-D-glucopyranosylunit (1-6) linked to a beta-D-glucopyranosyl unit of the linear main chain with an average branching degree of about 0.3, wherein the modification confers an increased activity -compared to a non-modified control microorganism of the same strain -of at least two gene products selected from the group consisting of (a) hexokinase, (b) phosphoglucomutase,. (c) UTP-glucose-1-phosphate uridyltrasferase, (d) 1,3–beta-glucan synthase (e) branching enzyme; under conditions allowing said microorganism to produce said polymer; (ii) optionally recovering said polymer from the medium.

摘要翻译： 用于生产由β-D-（1-3） - 吡喃葡萄糖基单元的线性主链组成的聚合物的方法，所述β-D-（1-3） - 吡喃葡萄糖基单元具有与线性的β-D-吡喃葡萄糖基单元连接的单个β-D-吡喃葡萄糖基单元 主链的平均支化度为约0.3，所述方法包括以下步骤：（i）在培养基中培养能够产生由β-D-（1-3）的线性主链组成的聚合物的基因修饰微生物， - 吡喃葡萄糖基单元，其具有与平均支化度为约0.3的线性主链的β-D-吡喃葡萄糖基单元连接的单一β-D-吡喃葡萄糖基单元（1-6），其中所述修饰赋予与 （a）己糖激酶，（b）磷酸葡萄糖变位酶，至少两种基因产物的相同菌株的未修饰的对照微生物。 （c）UTP-葡萄糖-1-磷酸尿苷转移酶，（d）1,3-β-葡聚糖合酶（e）分支酶; 在允许所述微生物产生所述聚合物的条件下; （ii）任选从介质中回收所述聚合物。