公开(公告)号：EP1283872B1

公开(公告)日：2009-10-07

申请号：EP01930379.1

申请日：2001-05-07

申请人： Modpro AB

发明人： BALTZER, Lars

IPC分类号： C12N9/10 ,  C07K1/10 ,  C07K14/00

CPC分类号： C07K1/1136 ,  C07K1/006 ,  C07K1/1072 ,  C07K1/1077

摘要： A catalytically active peptide comprising an imidazole function in position i flanked by at least one functional group to be amidated in position i+3+4k, where k is an integer equal to or higher than -1 or in position i-4-4n, wherein n is an integer equal to or higher than 0, characterized in that it also comprises at least one activating group in position i+4+4n or i-3-4n, respectively, wherein n is as above.

公开(公告)号：EP1631597A4

公开(公告)日：2007-07-11

申请号：EP04752449

申请日：2004-05-17

申请人： GRYPHON THERAPEUTICS INC

发明人： LOW DONALD ,  HU YING ,  BOTTI PAOLO

IPC分类号： C08F2/00 ,  A61K20060101 ,  C07K1/04 ,  C07K1/107 ,  C08B37/00

CPC分类号： C07K1/042 ,  C07K1/1072

摘要： The invention is directed to methods and compositions for aqueous-compatible solid phase chemical ligation. The methods and compositions of the invention involve the chemical ligation of first and second polymers, where the first polymer is attached to a support through a displaceable linker. The displaceable linker can be cleaved or otherwise displaced under aqueous conditions compatible with subsequent removal of the ligation product from the solid support. The methods and compositions of the invention are particularly useful for ligation of peptides and polypeptides on a solid support. The ligation system of the invention is applicable to a wide variety of molecules, and thus can be exploited to generate ligated polymers including peptides, polypeptides and other amino acid containing polymers.

公开(公告)号：EP1631597A2

公开(公告)日：2006-03-08

申请号：EP04752449.1

申请日：2004-05-17

申请人： Gryphon Therapeutics, Inc.

发明人： LOW, Donald ,  HU, Ying ,  BOTTI, Paolo

IPC分类号： C08F2/00 ,  C08B37/00

CPC分类号： C07K1/042 ,  C07K1/1072

摘要： The invention is directed to methods and compositions for aqueous-compatible solid phase chemical ligation. The methods and compositions of the invention involve the chemical ligation of first and second polymers, where the first polymer is attached to a support through a displaceable linker. The displaceable linker can be cleaved or otherwise displaced under aqueous conditions compatible with subsequent removal of the ligation product from the solid support. The methods and compositions of the invention are particularly useful for ligation of peptides and polypeptides on a solid support. The ligation system of the invention is applicable to a wide variety of molecules, and thus can be exploited to generate ligated polymers including peptides, polypeptides and other amino acid containing polymers.

公开(公告)号：EP1616021A2

公开(公告)日：2006-01-18

申请号：EP03814988.6

申请日：2003-12-30

申请人： Gryphon Therapeutics, Inc.

发明人： KOCHENDOERFER, Gerd, G. ,  SHAO, Haiyan ,  CRESSMAN, Sonya

IPC分类号： C12Q1/00 ,  G01N33/53 ,  C07K14/00

CPC分类号： C07K1/1077 ,  C07K1/023 ,  C07K1/047 ,  C07K1/1072 ,  C07K14/535 ,  Y02P20/55

摘要： The invention concerns a multiplex polymer ligation method involving water-soluble polymeric protecting groups, compositions and methods of use. The method involves synthesis of a first compound bearing one or more water-soluble polymeric protecting groups followed by replacing one or more of the water-soluble protecting groups with a chemical adduct of interest. Multiple different compounds derived from the first compound can be readily prepared by splitting the first compound into first and second reaction systems, followed by replacing one or more of the water-soluble polymeric protecting groups of the first reaction system with a first chemical adduct, and replacing one or more of the water-soluble polymeric protecting groups of the second reaction system with a second chemical adduct of interest, where the first and second chemical adducts are different. Also provided are kits useful for carrying out the multiplex polymer ligation methods of the invention.

公开(公告)号：EP1456226A1

公开(公告)日：2004-09-15

申请号：EP02783948.9

申请日：2002-11-18

申请人： ModPro AB

发明人： BALTZER, Lars

IPC分类号： C07K1/10 ,  C07K14/00

CPC分类号： C07K1/1072

摘要： Disclosed are novel polypeptides consisting of a four helix bundle formed of two dimerized helix-loop-helix motifs, wherein either both have a sequence according to SEQ. ID. No. 1, SEQ. ID. No. 2, SEQ. ID. No. 3, SEQ. ID. No. 4, SEQ. ID. No. 5 or SEQ. ID. No. 7, or one has a sequences according to SEQ. ID. No. 6, and the other one has a sequence according to SEQ. ID. No. 1, SEQ. ID. No. 2, SEQ. ID. No. 3, SEQ. ID. No. 4, SEQ. ID. No. 5 or SEQ. ID. No. 7. Also disclosed is a method for site-selective acylation of a folded polypeptide or protein based on the use of a four helix bundle formed of two dimerized helix-loop-helix motifs folded in an antiparallel mode, said helix-loop-helix motifs comprising amino acid residues in a heptad repeat pattern a b c d e fgn, wherein all but 1-3 of said amino acid residues in positions a and d are non-polar and wherein said 1-3 amino acid residues in positions a and d that are non-polar, are selected from the group consisting of lysine, ornithine, diaminobutyric acid and homolysine, wherein said amino acid residues in positions a and d form a hydrophobic core, wherein the polypeptide and/or protein to be acylated is placed in an aqueous solution and an acylation agent is added. Furthermore, use of said method is disclosed.

公开(公告)号：EP1399465A4

公开(公告)日：2004-03-31

申请号：EP01935432

申请日：2001-05-11

申请人： WISCONSIN ALUMNI RES FOUND

发明人： RAINES RONALD T ,  KIESSLING LAURA L ,  NILSSON BRADLEY L

IPC分类号： C07K1/06 ,  C07F9/48 ,  C07F9/50 ,  C07K1/08 ,  C07K1/107 ,  C07K1/12 ,  C07K1/13 ,  C07K1/00

CPC分类号： C07K1/082 ,  C07F9/4866 ,  C07F9/5004 ,  C07F9/5022 ,  C07F9/5054 ,  C07K1/1072 ,  C07K1/1077

摘要： Methods and reagents for the formation of amide bonds between an activated carboxylic acid derivative and an azide useful in the synthesis of peptides, proteins and derivatized or labeled amino acids, peptide or proteins. The method involves the formation of a phosphinothioester which reacts with an azide resulting in amide formation. The invention provides phosphinothiol reagents which convert activated carboxylic acid derivatives to phosphinothioesters which then react with azides to form an amide bond. The methods and reagents of the invention can be used for stepwise synthesis of peptides on solid supports or for the ligation to two or more amino acids, two or more peptide or two or more protein fragments.

摘要翻译： 用于在活性羧酸衍生物和叠氮化物之间形成酰胺键的方法和试剂，用于合成肽，蛋白质和衍生或标记的氨基酸，肽或蛋白质。 该方法包括形成与叠氮化物反应导致酰胺形成的膦硫代酯。 本发明提供了将活化的羧酸衍生物转化成膦酰硫酯的膦硫醇试剂，然后膦酰硫酯随后与叠氮化物反应形成酰胺键。 本发明的方法和试剂可用于在固体支持物上逐步合成肽或用于连接两个或更多个氨基酸，两个或更多个肽或两个或更多个蛋白质片段。

公开(公告)号：EP1399465A2

公开(公告)日：2004-03-24

申请号：EP01935432.3

申请日：2001-05-11

申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION

发明人： RAINES, Ronald, T. ,  KIESSLING, Laura, L. ,  NILSSON, Bradley, L.

IPC分类号： C07K1/00

CPC分类号： C07K1/082 ,  C07F9/4866 ,  C07F9/5004 ,  C07F9/5022 ,  C07F9/5054 ,  C07K1/1072 ,  C07K1/1077

摘要： Methods and reagents for the formation of amide bonds between an activated carboxylic acid derivative and an azide useful in the synthesis of peptides, proteins and derivatized or labeled amino acids, peptide or proteins. The method involves the formation of a phosphinothioester which reacts with an azide resulting in amide formation. The invention provides phosphinothiol reagents which convert activated carboxylic acid derivatives to phosphinothioesters which then react with azides to form an amide bond. The methods and reagents of the invention can be used for stepwise synthesis of peptides on solid supports or for the ligation to two or more amino acids, two or more peptide or two or more protein fragments.

公开(公告)号：EP1283872A2

公开(公告)日：2003-02-19

申请号：EP01930379.1

申请日：2001-05-07

申请人： A+ Science Invest AB

发明人： BALTZER, Lars

IPC分类号： C12N9/10 ,  C07K1/10 ,  C07K14/00

CPC分类号： C07K1/1136 ,  C07K1/006 ,  C07K1/1072 ,  C07K1/1077

摘要： A catalytically active peptide comprising an imidazole function in position i flanked by at least one functional group to be amidated in position i+3+4k, where k is an integer equal to or higher than -1 or in position i-4-4n, wherein n is an integer equal to or higher than 0, characterized in that it also comprises at least one activating group in position i+4+4n or i-3-4n, respectively, wherein n is as above.

公开(公告)号：EP0792357A4

公开(公告)日：1999-05-19

申请号：EP95938187

申请日：1995-09-29

申请人： PROTEIN POLYMER TECHNOLOGIES

发明人： STEDRONSKY ERWIN R

IPC分类号： C07K1/107 ,  C07K14/00 ,  C07K14/435 ,  C07K14/78 ,  C09D189/00 ,  C12N15/11

CPC分类号： C07K1/1072 ,  C07K14/001 ,  C07K14/435

摘要： Highly repetitive proteins which are relatively insoluble in water are chemically modified to increase solubility. The protein is reacted with a functionalizing agent to introduce additional polar functionalities and disrupt the order of the protein. The solubility of the protein in water is increased by the chemical modification, while adhesive and surfactant properties are retained.

公开(公告)号：EP0322262A1

公开(公告)日：1989-06-28

申请号：EP88402884.6

申请日：1988-11-17

申请人： COMMISSARIAT A L'ENERGIE ATOMIQUE

发明人： Menez, André ,  Chwetzoff, Serge

IPC分类号： C07K13/00 ,  C07K7/06 ,  C12N9/20 ,  G01N33/68 ,  A61K37/02

CPC分类号： C07K1/1072 ,  C07K14/46 ,  C12N9/18

摘要： Protéine basique isolée de venin de serpent de la famille des Elapidés notamment d'un serpent Naja et plus particulièrement de Naja nigricollis et/ou de Naja mossambica pallida, dénommée phospholipase A2 (PLA2), fragments et dérivés de ladite protéine, leurs procédés d'obtention, des compositions pharmaceutiques utilisables en médecine humaine et/ou vétérinaire, ainsi que des agents de diagnostic contenant ladite protéine et/ou ses dérivés et/ou ses fragments.
Ladite protéine comprend 118 amino-acides, son poids moleculaire est de l'ordre de 13 300 Daltons et son point isoélectrique est de l'ordre de 8,6.
Les dérivés de ladite protéine sont modifiés au niveau de l'une des histidines par fixation d'un groupe al­kyle de formule R-CH₂-X.
Application à la détection de cellules tumo­rales.

摘要翻译： 从家庭眼镜蛇科的蛇的毒液中分离的碱性蛋白质，特别是从一个眼镜蛇蛇和更爱特别是从眼镜蛇黑颈鹤和/或眼镜蛇罗非鱼淡紫，指定磷脂酶A2（PLA2），片段和该蛋白的衍生物，处理用于获得它们 和医药组合物，其在人类和/或兽用药物可用，以及诊断剂含有上述蛋白质和/或其衍生物和/或它的片段。 ... 上述的蛋白质包含118个氨基酸，其分子量为13,300道尔顿的数量级和其等电点为8.6的数量级。 ... 上述蛋白质的衍生物是通过结合于烷基的式R-CH 2 -X的基团在组氨酸的一个修改。 ... 在检测肿瘤细胞的使用。