公开(公告)号：EP0140713A3

公开(公告)日：1987-05-20

申请号：EP84307522

申请日：1984-10-30

申请人： GENEX CORPORATION

发明人： Schruber, Jeffrey John ,  Vollmer, Patricia Jean ,  Montgomery, John Patrick

IPC分类号： C12P13/22 ,  C12N09/88

CPC分类号： C12P13/222

摘要： A method for the production of L-phenylalanine which comprises combining t-cinnamic acid and ammonium ions in the presence of phenylalanine ammonia-lyase under L-phenylalanine-producing conditions and recovering the L-phenylalanine from the bioreaction mixture so produced characterised in that the source of ammonium ions comprises ammonium carbamate.

摘要翻译： 一种生产L-苯丙氨酸的方法，其包括在L-苯丙氨酸生产条件下在苯丙氨酸氨裂解酶存在下将叔肉桂酸和铵离子结合，并从如此生产的生物反应混合物中回收L-苯丙氨酸，其特征在于， 铵离子源包括氨基甲酸铵。

公开(公告)号：EP0136996A3

公开(公告)日：1987-04-01

申请号：EP84870129

申请日：1984-09-04

申请人： Monsanto Company

发明人： Kishore, Ganesh Murphy

IPC分类号： C12N09/88 ,  C12N09/96 ,  C12P13/22 ,  C12N11/10

CPC分类号： C12N11/10 ,  C12N9/88 ,  C12N9/96 ,  C12P13/222

摘要： A process for the synthesis of L-phenylalanines and analogues thereof from trans-cinnamate, ring substituted trans-cinnamates or various acrylate derivatives and ammonia or an ammonia donor in the presence of L-phenylalanine ammonia-lyase enzyme and a desensitizing agent such as polyhydric alcohols and polyethylene glycol-(400) is disclosed. The process embraces the discovery that compounds such as polyhydric alcohols and polyethylene glycol-(400) desensitize the L-phenyalanine ammonia-lyase enzyme to substantially higher substrate concentrations than practiced heretofore. In addition, polyhydric alcohols and polyethylene glycol-(400) substantially enhance the instantaneous rate of reaction and inhibit inactivation of the L-phenylalanine ammonia lyase enzyme over longer reaction periods.

公开(公告)号：EP0138526A3

公开(公告)日：1987-03-04

申请号：EP84306803

申请日：1984-10-05

申请人： AJINOMOTO CO., INC.

发明人： Tsuchida, Takayasu ,  Kurahashi, Osamu ,  Kawashima, Hiroki ,  Enei, Hitoshi

IPC分类号： C12P13/22

CPC分类号： C12R1/125 ,  C12P13/222

摘要： L-Tryptophan and L-phenylalanine auxotrophic mutant strains of the genus Bacillus which are capable of vigorous growth on culture medium containing enough of m-fluorophenylalanine and/or cinnamic acid to suppress growth of the parent strain produce more L-phenylalanine by aerobic fermentation of generally conventional aqueous media than the best L-phenylalanine producing related microorganisms available heretofore.

公开(公告)号：EP0206687A1

公开(公告)日：1986-12-30

申请号：EP86304566

申请日：1986-06-13

申请人： NOVO INDUSTRI AS

发明人： WUEMPELMANN MOGENS

IPC分类号： C12N9/88 ,  C12N11/08 ,  C12P13/22 ,  C12P19/24 ,  C12R1/19 ,  C12R1/465

CPC分类号： C12P19/24 ,  C12N9/88 ,  C12N11/08 ,  C12P13/227

摘要： An improvement directed to the generation of particle form cell mass immobilized enzymes through cross-linking with polyazetidine prepolymer which comprises pretreating the enzymatically active microorganism cells in aqueous suspension with glutaraldehyde to effect partial cross-linking followed by dewatering and then admixing so-treated cell mass with the polyazetidine prepolymer followed by curing. … The dispersion of treated enzymatically active cell mass with the polyazetidine prepolymer may be sub-divided and shaped into granular form prior to curing, so that the immobilized enzyme product comprises cell mass enzyme granules of desired size and a controlled shape in high yield. … Glucose isomerase from a strain of the Streptomyces murinus cluster and tryptophan synthetase from a strain of E. coli are exemplified preferred embodiments.

摘要翻译： 通过与polyazetidine预聚物，其包括预处理所述酶促活性的微生物细胞wässrige悬浮液用戊二醛进行部分交联通过脱水，然后混合如此处理的细胞团随后交联涉及颗粒形状细胞块固定化酶的产生的改进 与预聚物polyazetidine通过固化其次。 ... 治疗酶促活性细胞块与polyazetidine预聚物可以被细分并成型为颗粒形式在固化前的分散体，所以也固定化酶产物包含期望的尺寸和在受控的形状的细胞块酶颗粒 产量高。 ...从链霉菌鼱簇和色氨酸合成酶的来自大肠杆菌的菌株例举优选的实施方式的应变葡萄糖异构酶。

公开(公告)号：EP0190921A2

公开(公告)日：1986-08-13

申请号：EP86300748.0

申请日：1986-02-04

申请人： ENGENICS, INC.

发明人： Talkington, Carol

IPC分类号： C12N15/00 ,  C12P13/04 ,  C12P13/22

CPC分类号： C12P13/04 ,  C12N15/52 ,  C12N15/70 ,  C12P13/22

摘要： A method is provided for overexpressing an amino acid in a microorganism comprising the step of altering the chromosomal DNA of host microorganisms or transforming the host microorganisms with at least one expression vector such that the resultant microorganism will overexpress certain operons and be inactive with respect to the other operons in the biosynthetic pathway of the amino acid, whereby overexpression of the amino acid will occur.

摘要翻译： 提供了用于过表达微生物中的氨基酸的方法，其包括改变宿主微生物的染色体DNA或用至少一种表达载体转化宿主微生物的步骤，使得所得微生物将过表达某些操纵子，并相对于 氨基酸生物合成途径中的其他操纵子，由此会发生氨基酸的过度表达。

公开(公告)号：EP0180192A1

公开(公告)日：1986-05-07

申请号：EP85113730.7

申请日：1985-10-29

申请人： RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL

发明人： Yukawa, Hideaki ,  Terasawa, Masato ,  Kurusu, Yasuro ,  Shimazu, Mitsunobu

IPC分类号： C12N15/60 ,  C12N15/68 ,  C12N15/69 ,  C12N1/20 ,  C12P13/22

CPC分类号： C12N15/68 ,  C12N9/88 ,  C12N15/69 ,  C12P13/227

摘要： A novel plasmid which can be autonomously replicated within the cells of Escherichia coli, said plasmid comprising a DNA fragment containing genes which govern the biosynthesis of tryptophan synthase, a DNA fragment containing genes which are derived from F-plasmid and govern its partition system and a DNA fragment containing genes which are derived from a Col El-type plasmid and govern its autonomous replication. A host organism transformed with the plasmid is useful for industrial production of tryptophan.

摘要翻译： 一种能够在大肠杆菌细胞内自主复制的新型质粒，所述质粒包含含有控制色氨酸合成酶生物合成的基因的DNA片段，含有源于F-质粒的基因并控制其分配系统的DNA片段和 含有衍生自Col El型质粒并控制其自主复制的基因的DNA片段。 用质粒转化的宿主生物体可用于工业生产色氨酸。

公开(公告)号：EP0178553A2

公开(公告)日：1986-04-23

申请号：EP85112563.3

申请日：1985-10-04

申请人： HOECHST AKTIENGESELLSCHAFT

发明人： Keller, Reinhold, Dr. ,  Schlingmann, Merten, Dr.

IPC分类号： C12P13/22 ,  C12P41/00 ,  C07B55/00

CPC分类号： C12P13/22 ,  C12P41/005

摘要： Aromatisch substituierte L-Aminosäuren werden besonders vorteilhaft durch enzymatische Spaltung des Racemats der niederen Alkylester mit a-Chymotrypsin, Extraktion des D-Esters aus dem alkalisch gestellten Reaktionsgemisch mit einem organischen Lösemittel, Racemisierung, Extraktion des D,L-Esters mit wäßriger Säure und Rückführung nach Aufstärken in den Prozeß sowie Isolierung der L-Aminosäure aus dem alkalischen Extraktionsrückstand nach Ansäuern erhalten.Das Enzym wird vorteilhaft an einen anorganischen Träger fixiert. Die Racemisierung erfolgt zweckmäßig im Extrakt, also ohne Isolierung des D-Esters.

摘要翻译： 1.通过在弱酸性pH范围内使用α-胰凝乳蛋白酶酶解裂解低级烷基酯（叔丁基酯除外）的外消旋物并制备芳香取代的L-氨基酸的方法，并提取D的酯 形式，其包含在酶裂解中获得的反应混合物为弱碱性，使用有机溶剂从该碱性溶液中提取D-氨基酸的酯，并进行外消旋化，然后将外消旋体返回到该方法中， 将提取的碱性溶液变成弱酸，随后从其中分离出L-氨基酸。

公开(公告)号：EP0170257A2

公开(公告)日：1986-02-05

申请号：EP85109579.4

申请日：1985-07-30

申请人： AJINOMOTO CO., INC.

发明人： Ishida, Masaaki ,  Miwa, Kiyoshi ,  Nakamori, Shigeru ,  Sano, Konosuke

IPC分类号： C12N15/60 ,  C12P13/22 ,  C12N1/21

CPC分类号： C12P13/227 ,  C12N9/88 ,  C12N15/77

摘要： A process for producing L-tryptophan in which a suitable substrate such as a carbohydrate, indole or anthranilic acid is contacted with Coryneform bacteria, where the Coryneform bacteria bear recombinant DNA constructed by connecting a gene coding for tryptophan synthetase with a plasmid vector capable of proliferating in Coryneform bacteria.

公开(公告)号：EP0159866A2

公开(公告)日：1985-10-30

申请号：EP85302548.4

申请日：1985-04-11

申请人： DENKI KAGAKU KOGYO KABUSHIKI KAISHA

发明人： Miyoshi, Teruzo ,  Kitagawa, Hironoshin ,  Kato, Masaaki ,  Chiba, Susumu DENKI KAGAKU KOGYO K.K.

IPC分类号： C12P13/22 ,  C12P13/04 ,  C12N1/20

CPC分类号： C12R1/06 ,  C12P13/04 ,  C12P13/22

摘要： L-amino acids are produced by means of an microbial enzyme system by treating a 5-substituted hydantoin or an N-carbamyl derivative of an amino acid with cells or the contents of cells of a strain which belongs to the genus Arthrobacter and which is capable of converting the 5-substituted hydantoin or N-carbamyl derivative into a corresponding L-amino acid.

摘要翻译： 通过微生物酶系统通过用属于节杆菌属的菌株的细胞或细胞的含量处理氨基酸的5-取代的乙内酰脲或N-氨基甲酰基衍生物来产生L-氨基酸，并且其能够 将5-取代的乙内酰脲或N-氨基甲酰基衍生物转化为相应的L-氨基酸。

公开(公告)号：EP0156504A1

公开(公告)日：1985-10-02

申请号：EP85301161.7

申请日：1985-02-21

申请人： KYOWA HAKKO KOGYO KABUSHIKI KAISHA

发明人： Kino, Kuniki ,  Sugimoto, Masahiro ,  Nakanishi, Toshihide

IPC分类号： C12P13/22

CPC分类号： C12R1/15 ,  C12P13/227 ,  Y10S435/843

摘要： L-tryptophan can be prepared in good yield by a fermentation process which comprises culturing a novel L-tryptophan-producing microorganism of the genus Corynebacterium, which is resistant to at least one member selected from glyphosate [N-phosphonomethyl glycine], paraquat [1,1'-dimethyl-4,4'-bispyridinium] and derivatives thereof, and recovering L-tryptophan from the culture broth.